BioE MS thesis defense presentation- Fredrick Bulondo

Event Details
  • Date/Time:
    • Thursday July 21, 2022
      12:00 pm - 2:00 pm
  • Location: 1128 IBB & https://gatech.zoom.us/j/93462208751?pwd=RDlrcmdZY0FaWHFsemRNdk9idUVvdz09
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Summaries

Summary Sentence: "OPTIMIZATION OF IL-10 INCORPORATION FOR DENDRITIC CELLS EMBEDDED IN PEG-4MAL HYDROGELS. "

Full Summary: BioE MS thesis defense presentation- "OPTIMIZATION OF IL-10 INCORPORATION FOR DENDRITIC CELLS EMBEDDED IN PEG-4MAL HYDROGELS. " - Fredrick Bulondo

Advisor:  

Julia E. Babensee, PhD | School of Biomedical Engineering, Georgia Institute of Technology. 

 

Committee: 

Susan Thomas, PhD | School of Mechanical Engineering, Georgia Institute of Technology. 

Stanislav Emelianov, PhD | School of Electrical & Computer Engineering, Georgia Institute of Technology. 

 

OPTIMIZATION OF IL-10 INCORPORATION FOR DENDRITIC CELLS Embedded IN PEG-4MAL HYDROGELS. 

 

Abstract 

Over the recent decades, translational research in biomaterials and immunoengineering has been appreciated by science, which is corroborated by the development of novel advanced therapies to treat cancer, autoimmunity, and other immune-related pathologies. Dendritic cells (DCs) have been at the core center of pharmaceutical and biological therapeutics as vital mediator of the immune system leveraging on its function to bridge the innate and adaptive immune system. This thesis focuses on developing a biomaterial system to ameliorate autoimmunity.  This biomaterial system is comprised of a poly (ethylene glycol)- 4 arms maleimide (PEG-4MAL) hydrogels conjugated with the immunosuppressive cytokine, interleukin, IL-10, which is injectable, in situ cross linkable and degradable system for localized delivery of immunosuppressive DCs. Studies conducted here aimed at optimizing the amount of IL-10 incorporated in hydrogel at 500ng concentration, which exhibited highest DC viability, immunosuppressive phenotype and protection against pro-inflammatory insult as compared to hydrogel-incorporated DCs at lower loading IL-10 amounts. Additionally, the studies addressed the optimization of degradability of the hydrogel to control the release rate of IL-10 from the gel, by varying the ratio of adhesive peptides: VPM (degradable) and DTT (non-degradable) peptide crosslinkers. The results obtained are promising and shall be significant for in vivo model optimization of immunosuppressive viability and functionality for incorporated DCs in cell delivery immunotherapy function. 

Henceforth, it important to incorporate optimal loading amounts of IL-10 with hydrogels embedding DCs because this immunosuppressive cytokine provides a tolerogenic environment that keeps DCs in their immature phenotype which consequently enhances cell viability and optimizes the system’s immune modulatory functionality.  

 

Additional Information

In Campus Calendar
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Groups

Bioengineering Graduate Program

Invited Audience
Faculty/Staff, Public, Undergraduate students
Categories
Career/Professional development
Keywords
go-BioE
Status
  • Created By: Laura Paige
  • Workflow Status: Published
  • Created On: Jul 20, 2022 - 10:25am
  • Last Updated: Jul 20, 2022 - 10:25am