{"659495":{"#nid":"659495","#data":{"type":"event","title":"BioE MS thesis defense presentation- Fredrick Bulondo","body":[{"value":"\u003Cp\u003E\u003Cstrong\u003EAdvisor:\u003C\/strong\u003E \u0026nbsp;\u003C\/p\u003E\r\n\r\n\u003Cp\u003EJulia E. Babensee, PhD | School of Biomedical Engineering, \u003Cem\u003EGeorgia Institute of Technology.\u0026nbsp;\u003C\/em\u003E\u003C\/p\u003E\r\n\r\n\u003Cp\u003E\u0026nbsp;\u003C\/p\u003E\r\n\r\n\u003Cp\u003E\u003Cstrong\u003ECommittee:\u0026nbsp;\u003C\/strong\u003E\u003C\/p\u003E\r\n\r\n\u003Cp\u003ESusan Thomas, PhD | School of Mechanical Engineering, \u003Cem\u003EGeorgia Institute of Technology.\u0026nbsp;\u003C\/em\u003E\u003C\/p\u003E\r\n\r\n\u003Cp\u003EStanislav Emelianov, PhD | School of Electrical \u0026amp; Computer Engineering, \u003Cem\u003EGeorgia Institute of Technology.\u0026nbsp;\u003C\/em\u003E\u003C\/p\u003E\r\n\r\n\u003Cp\u003E\u0026nbsp;\u003C\/p\u003E\r\n\r\n\u003Cp\u003E\u003Cstrong\u003EOPTIMIZATION OF IL-10 INCORPORATION FOR DENDRITIC CELLS Embedded IN PEG-4MAL HYDROGELS.\u003C\/strong\u003E\u0026nbsp;\u003C\/p\u003E\r\n\r\n\u003Cp\u003E\u0026nbsp;\u003C\/p\u003E\r\n\r\n\u003Cp\u003E\u003Cstrong\u003EAbstract\u0026nbsp;\u003C\/strong\u003E\u003C\/p\u003E\r\n\r\n\u003Cp\u003EOver the recent decades, translational research in biomaterials and immunoengineering has been appreciated by science, which is corroborated by the development of novel advanced therapies to treat cancer, autoimmunity, and other immune-related pathologies. Dendritic cells (DCs) have been at the core center of pharmaceutical and biological therapeutics as vital mediator of the immune system leveraging on its function to bridge the innate and adaptive immune system. This thesis focuses on developing a biomaterial system to ameliorate autoimmunity.\u0026nbsp; This biomaterial system is comprised of a poly (ethylene glycol)- 4 arms maleimide (PEG-4MAL) hydrogels conjugated with the immunosuppressive cytokine, interleukin, IL-10, which is injectable, \u003Cem\u003Ein situ\u003C\/em\u003E cross linkable and degradable system for localized delivery of immunosuppressive DCs. Studies conducted here aimed at optimizing the amount of IL-10 incorporated in hydrogel at 500ng concentration, which exhibited highest DC viability, immunosuppressive phenotype and protection against pro-inflammatory insult as compared to hydrogel-incorporated DCs at lower loading IL-10 amounts. Additionally, the studies addressed the optimization of degradability of the hydrogel to control the release rate of IL-10 from the gel, by varying the ratio of adhesive peptides: VPM (degradable) and DTT (non-degradable) peptide crosslinkers. The results obtained are promising and shall be significant for \u003Cem\u003Ein vivo\u003C\/em\u003E model optimization of immunosuppressive viability and functionality for incorporated DCs in cell delivery immunotherapy function.\u0026nbsp;\u003C\/p\u003E\r\n\r\n\u003Cp\u003EHenceforth, it important to incorporate optimal loading amounts of IL-10 with hydrogels embedding DCs because this immunosuppressive cytokine provides a tolerogenic environment that keeps DCs in their immature phenotype which consequently enhances cell viability and optimizes the system\u0026rsquo;s immune modulatory functionality.\u0026nbsp;\u0026nbsp;\u003C\/p\u003E\r\n\r\n\u003Cp\u003E\u0026nbsp;\u003C\/p\u003E\r\n","summary":null,"format":"limited_html"}],"field_subtitle":"","field_summary":[{"value":"\u003Cp\u003EBioE MS thesis defense presentation- \u0026quot;OPTIMIZATION OF IL-10 INCORPORATION FOR DENDRITIC CELLS EMBEDDED IN PEG-4MAL HYDROGELS. \u0026quot; - Fredrick Bulondo\u003C\/p\u003E\r\n","format":"limited_html"}],"field_summary_sentence":[{"value":"\u0022OPTIMIZATION OF IL-10 INCORPORATION FOR DENDRITIC CELLS EMBEDDED IN PEG-4MAL HYDROGELS. \u0022"}],"uid":"27917","created_gmt":"2022-07-20 14:25:34","changed_gmt":"2022-07-20 14:25:34","author":"Laura Paige","boilerplate_text":"","field_publication":"","field_article_url":"","field_event_time":{"event_time_start":"2022-07-21T13:00:00-04:00","event_time_end":"2022-07-21T15:00:00-04:00","event_time_end_last":"2022-07-21T15:00:00-04:00","gmt_time_start":"2022-07-21 17:00:00","gmt_time_end":"2022-07-21 19:00:00","gmt_time_end_last":"2022-07-21 19:00:00","rrule":null,"timezone":"America\/New_York"},"extras":[],"groups":[{"id":"65448","name":"Bioengineering Graduate Program"}],"categories":[],"keywords":[{"id":"172056","name":"go-BioE"}],"core_research_areas":[],"news_room_topics":[],"event_categories":[{"id":"10377","name":"Career\/Professional development"}],"invited_audience":[{"id":"78761","name":"Faculty\/Staff"},{"id":"78771","name":"Public"},{"id":"78751","name":"Undergraduate students"}],"affiliations":[],"classification":[],"areas_of_expertise":[],"news_and_recent_appearances":[],"phone":[],"contact":[],"email":[],"slides":[],"orientation":[],"userdata":""}}}