BioE PhD Proposal- Michelle Quizon

Event Details
  • Date/Time:
    • Tuesday May 31, 2022
      11:00 am - 1:00 pm
  • Location: IBB 1128 (Suddath Seminar Room) Zoom: https://gatech.zoom.us/j/98587638140
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Summaries

Summary Sentence: "Synthetic hydrogels for islet vascularization and engraftment in the subcutaneous space"

Full Summary: BioE PhD Proposal- "Synthetic hydrogels for islet vascularization and engraftment in the subcutaneous space"-  Michelle Quizon

Advisor:

Andrés J. García, Ph.D. (Georgia Institute of Technology)

Thesis Committee:

Edward A. Botchwey, Ph.D. (Georgia Institute of Technology & Emory University)

Rebecca D. Levit, M.D. (Emory University)

Edward A. Phelps, Ph.D. (University of Florida)

Krishnendu Roy, Ph.D. (Georgia Institute of Technology & Emory University)

 

 

Synthetic hydrogels for islet vascularization and engraftment in the subcutaneous space

 

                Type 1 diabetes (T1D) is a chronic, debilitating disease  characterized by the autoimmune destruction of insulin-producing b-cells located within pancreatic islets. The gold standard for T1D cell therapy is clinical islet transplantation (CIT), the infusion of islets through the hepatic portal vein. While CIT recipients demonstrate enhanced blood glucose control,  the procedure is limited to a marginal subset of T1D patients, in part due to the inhospitable nature of the intrahepatic site Indeed, an expected >60% loss of therapeutic cargo is expected within three days following transplantation. Thus, there is a significant need to establish an alternative extrahepatic transplant site that supports the engraftment of islets.

The subcutaneous space is an attractive extrahepatic transplant site for T1D cell therapy given its high clinical potential in terms of surgical accessibility, ease of monitoring, and convenience for replenishment and/or retrieval of therapeutic cargo. However, the unmodified subcutaneous space lacks adequate vascularization necessary to preserve functional islets. An elegant, facile strategy to promote neovascularization is the biomaterial-mediated delivery of proangiogenic factors such as vascular endothelial growth factor (VEGF). The objective of this project is to engineer injectable VEGF-delivering synthetic poly(ethylene glycol) [PEG] hydrogels that promote islet vascularization, engraftment, and function in the subcutaneous space. My central hypothesis is that the VEGF-delivering hydrogel can be tuned to do so. 

To test my hypothesis, I will first identify VEGF-PEG hydrogel formulations that support islet vascularization using an in vitro platform of vascularized islets. Next, I will evaluate lead VEGF-PEG hydrogel formulations in their ability to promote allogeneic islet vascularization, engraftment, and function in the subcutaneous spaces of diabetic rats and nondiabetic pigs. My work will result in an optimized injectable hydrogel for islet vascularization, engraftment, and function. Most significantly, it will provide a solid foundation for future work in a translational diabetic large animal model.

Additional Information

In Campus Calendar
No
Groups

Bioengineering Graduate Program

Invited Audience
Faculty/Staff, Public, Undergraduate students
Categories
Career/Professional development
Keywords
go-BioE
Status
  • Created By: Laura Paige
  • Workflow Status: Published
  • Created On: May 13, 2022 - 10:12am
  • Last Updated: May 13, 2022 - 10:12am