PhD Proposal by Nate Dwarshuis

Event Details
  • Date/Time:
    • Tuesday May 7, 2019 - Wednesday May 8, 2019
      12:00 pm - 1:59 pm
  • Location: EBB 5029
  • Phone:
  • URL:
  • Email:
  • Fee(s):
  • Extras:
No contact information submitted.

Summary Sentence: Optimizing T Cell Manufacturing and Quality Using Functionalized Microcarriers

Full Summary: No summary paragraph submitted.

Nate Dwarshuis

BME PhD Proposal Presentation


Date: May 7th, 2019

Time: 12:00 PM

Location: EBB 5029


Advisor: Krishnendu Roy (Georgia Institute of Technology)


Committee Members:

Madhav Dhodapkar (Emory University)

Melissa Kemp (Georgia Institute of Technology)

Wilbur Lam (Georgia Institute of Technology)

Sakis Mantalaris (Georgia Institute of Technology)


Title: Optimizing T Cell Manufacturing and Quality Using Functionalized Microcarriers




Adoptive cell therapies (ACT) using chimeric antigen receptor (CAR) T cells have shown promise in treating cancer, but manufacturing large numbers of high quality cells remains challenging. Critically, current T cell expansion technologies only partially recapitulate the in vivo microenvironment found in the human lymph nodes. In these organs, T cells expand at high cell density with autocrine/paracrine signaling, as well as signals from the extracellular matrix (ECM). Here we propose a T cell expansion system using degradable gelatin microcarriers functionalized with anti-CD3 and anti-CD28 monoclonal antibodies (mAbs), which address several of these shortcomings by providing a 3D surface on which T cells can cluster at high cell density and remain in contact with ECM components.


The overall objective of this proposal will be to develop this microcarrier platform into a controllable process that can conceivably be used in industry. We will do this in three aims: 1) Develop a novel microcarrier platform and assess its performance relative to current state-of-the-art T cell expansion technology, 2) optimize the microcarrier system for in vivo performance using a design of experiments (DOE) approach, and 3) characterize microcarrier- expanded T cell of differing performance using multiomics techniques to generate predictive models. The result of these three aims will be a platform for expanding T cells and an accompanying set of mathematical models that can be used to predict and control process outcomes.



Additional Information

In Campus Calendar

Graduate Studies

Invited Audience
Faculty/Staff, Public, Graduate students, Undergraduate students
Phd proposal
  • Created By: Tatianna Richardson
  • Workflow Status: Published
  • Created On: Apr 25, 2019 - 12:29pm
  • Last Updated: Apr 25, 2019 - 12:29pm