{"620922":{"#nid":"620922","#data":{"type":"event","title":"PhD Proposal by Nate Dwarshuis","body":[{"value":"\u003Cp\u003ENate Dwarshuis\u003C\/p\u003E\r\n\r\n\u003Cp\u003EBME PhD Proposal Presentation\u003C\/p\u003E\r\n\r\n\u003Cp\u003E\u0026nbsp;\u003C\/p\u003E\r\n\r\n\u003Cp\u003EDate: May 7th, 2019\u003C\/p\u003E\r\n\r\n\u003Cp\u003ETime: 12:00 PM\u003C\/p\u003E\r\n\r\n\u003Cp\u003ELocation: EBB 5029\u003C\/p\u003E\r\n\r\n\u003Cp\u003E\u0026nbsp;\u003C\/p\u003E\r\n\r\n\u003Cp\u003EAdvisor: Krishnendu Roy (Georgia Institute of Technology)\u003C\/p\u003E\r\n\r\n\u003Cp\u003E\u0026nbsp;\u003C\/p\u003E\r\n\r\n\u003Cp\u003ECommittee Members:\u003C\/p\u003E\r\n\r\n\u003Cp\u003EMadhav Dhodapkar (Emory University)\u003C\/p\u003E\r\n\r\n\u003Cp\u003EMelissa Kemp (Georgia Institute of Technology)\u003C\/p\u003E\r\n\r\n\u003Cp\u003EWilbur Lam (Georgia Institute of Technology)\u003C\/p\u003E\r\n\r\n\u003Cp\u003ESakis\u0026nbsp;Mantalaris (Georgia Institute of Technology)\u003C\/p\u003E\r\n\r\n\u003Cp\u003E\u0026nbsp;\u003C\/p\u003E\r\n\r\n\u003Cp\u003ETitle: Optimizing T Cell Manufacturing and Quality Using Functionalized Microcarriers\u003C\/p\u003E\r\n\r\n\u003Cp\u003E\u0026nbsp;\u003C\/p\u003E\r\n\r\n\u003Cp\u003EAbstract\/Summary:\u003C\/p\u003E\r\n\r\n\u003Cp\u003E\u0026nbsp;\u003C\/p\u003E\r\n\r\n\u003Cp\u003EAdoptive cell therapies (ACT) using chimeric antigen receptor (CAR) T cells have shown promise in treating cancer, but manufacturing large numbers of high quality cells remains challenging. Critically, current T cell expansion technologies only partially recapitulate the in vivo microenvironment found in the human lymph nodes. In these organs, T cells expand at high cell density with autocrine\/paracrine signaling, as well as signals from the extracellular matrix (ECM). Here we propose a T cell expansion system using degradable gelatin microcarriers functionalized with anti-CD3 and anti-CD28 monoclonal antibodies (mAbs), which address several of these shortcomings by providing a 3D surface on which T cells can cluster at high cell density and remain in contact with ECM components.\u003C\/p\u003E\r\n\r\n\u003Cp\u003E\u0026nbsp;\u003C\/p\u003E\r\n\r\n\u003Cp\u003EThe overall objective of this proposal will be to develop this microcarrier platform into a controllable process that can conceivably be used in industry. We will do this in three aims: 1) Develop a novel microcarrier platform and assess its performance relative to current state-of-the-art T cell expansion technology, 2) optimize the microcarrier system for in vivo performance using a design of experiments (DOE) approach, and 3) characterize microcarrier- expanded T cell of differing performance using multiomics techniques to generate predictive models. The result of these three aims will be a platform for expanding T cells and an accompanying set of mathematical models that can be used to predict and control process outcomes.\u003C\/p\u003E\r\n\r\n\u003Cp\u003E\u0026nbsp;\u003C\/p\u003E\r\n\r\n\u003Cp\u003E\u0026nbsp;\u003C\/p\u003E\r\n","summary":null,"format":"limited_html"}],"field_subtitle":"","field_summary":"","field_summary_sentence":[{"value":"Optimizing T Cell Manufacturing and Quality Using Functionalized Microcarriers"}],"uid":"27707","created_gmt":"2019-04-25 16:29:05","changed_gmt":"2019-04-25 16:29:05","author":"Tatianna Richardson","boilerplate_text":"","field_publication":"","field_article_url":"","field_event_time":{"event_time_start":"2019-05-07T13:00:00-04:00","event_time_end":"2019-05-07T15:00:00-04:00","event_time_end_last":"2019-05-07T15:00:00-04:00","gmt_time_start":"2019-05-07 17:00:00","gmt_time_end":"2019-05-07 19:00:00","gmt_time_end_last":"2019-05-07 19:00:00","rrule":null,"timezone":"America\/New_York"},"extras":[],"groups":[{"id":"221981","name":"Graduate Studies"}],"categories":[],"keywords":[{"id":"102851","name":"Phd proposal"}],"core_research_areas":[],"news_room_topics":[],"event_categories":[{"id":"1788","name":"Other\/Miscellaneous"}],"invited_audience":[{"id":"78761","name":"Faculty\/Staff"},{"id":"78771","name":"Public"},{"id":"174045","name":"Graduate students"},{"id":"78751","name":"Undergraduate students"}],"affiliations":[],"classification":[],"areas_of_expertise":[],"news_and_recent_appearances":[],"phone":[],"contact":[],"email":[],"slides":[],"orientation":[],"userdata":""}}}