Cancer Cell Motility in 3D

Event Details
  • Date/Time:
    • Thursday October 14, 2010 - Friday October 15, 2010
      11:00 am - 11:59 am
  • Location: IBB Suddath Room 1128
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James Godard
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Summary Sentence: BioE Seminar: Denis Wirtz, Ph.D. - Johns Hopkins University

Full Summary: Cancer Cell Motility in 3D

Denis Wirtz, Ph.D. - Johns Hopkins University - Bioengineering Seminar Series - October 14, 2010 11:00 AM - 12:00 PM, IBB Suddath Room 1128


Focal adhesions are large multi-protein clustered assemblies that form at the basal surface of cells placed on planar dishes which mediate cell signaling, force transduction, and adhesion with the underlying substratum. While much is known about the organization and function of focal adhesion components in 2-D systems, their organization and function in migrating cells within a more physiological three-dimensional (3-D) matrix is largely unknown. Quantitative live-cell microscopy shows that for cells fully embedded in a 3-D matrix focal adhesion proteins, including vinculin, paxillin, talin, -actinin, zyxin, VASP, FAK, and p130Cas, do not cluster into appreciable aggregates, but are diffusively distributed in the cytoplasm of cells. Despite the absence of detectable focal adhesions, focal adhesion proteins still modulate cell motility but in a manner distinct from cells moving on conventional planar substrates. Rather, focal adhesion proteins in matrix-embedded cells regulate cell speed by affecting protrusion activity and matrix deformation, two processes that play no direct role in controlling 2-D cell speed. This study shows that actively growing membrane protrusions constitute a critical motility/matrix-traction module that drives cell motility in a 3-D matrix. We will discuss the implications of this work in cancer metastasis.

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In Campus Calendar

Parker H. Petit Institute for Bioengineering and Bioscience (IBB)

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bioengineering, IBB, seminar
  • Created By: Alyceson Andrews
  • Workflow Status: Published
  • Created On: Sep 23, 2010 - 8:00pm
  • Last Updated: Oct 7, 2016 - 9:56pm