The objective of this proposed research is to conduct a scientific and engineering exploration into the real-time electrochemistry of single-stranded DNA (ssDNA) binding interactions during hybridization. The approach of this proposal is to create an amperometric biosensor to capture a transient current signal and interrogate this signature in simultaneous- or post-processing for features that can be proven to be directly due to the nucleotide base pair binding interactions during the zippering process. It is the hypothesis of this proposal that this can enable assessments of base pair mismatches between a target and probe strands. If successful, this device, and the attendant electronics, could be leveraged for biosensors used for miRNA detection in medical applications or as an error correction code methodology for Molecular Electronics where data is being stored in DNA. The result of this research effort will be the construction and validation of hardware and a microfluidic system that will be capable of future investigations DNA hybridization.