Jeonghwan (Dylan) Son
BME PhD Proposal Presentation

Date:2022-04-19
Time: 12:00 PM-1:30 PM EST
Location / Meeting Link: https://zoom.us/j/8194535482?pwd=UzVLSzcxdnVsNEtsWklWMFpWUGY5dz09 / Meeting ID 819 453 5482 / Passcord KUEYw6

Committee Members:
Shu Jia, Ph.D. (Advisor), Francisco Robles, Ph.D., Sungjin Park, Ph.D., Wilbur Lam, MD/Ph.D., Young Jang, Ph.D.


Title: Portable Open-top Light-sheet Microscopy (pOTLS): instrumentation, optofluidics, and applications

Abstract: Fluorescence optofluidic imaging is an emerging technique providing high-throughput imaging of biological systems with advantages of fluorescence. The recent advances in microscopy and micro-fabrication techniques have further pushed the inherent limits of image acquisition. These approaches, however, are still limited for 3D-volumetric imaging. The open-top light-sheet microscopy (OTLS) is a new variation of light-sheet microscopic configuration that provides 3Dimaging capabilities enabled by tilted excitation. Up to date, the implementation of OTLS on optofluidic imagining has not yet been fully investigated, particularly for high throughput screening of single cell levels. In the preliminary research, we have developed a portable open-top light-sheet microscopy (pOTLS) with a single cell resolution (<3 um laterally and <4 um axially). The system has demonstrated to fully reconstruct 3D-volumetric information of fluorescence beads. However, the limited demonstration and capability of the system still hampers the ability to investigate complex structural and functional information in optofluidic imaging schemes with single-cell resolution. Hence, we propose to advance the system by the below aims. Aim 1: Establish optofluidic imaging system and new imaging pipelines Aim 1-1. Instrument optofluidic imaging system Aim 1-2. Develop new algorithm to reconstruct volumetric information in optofluidic imaging Aim 1-3. Develop new analysis pipelines for biological studies Aim 2: Introduce simultaneous dual-color imaging channels Aim 2-1. Instrument dual-color imaging channels Aim 2-2. Characterize the performance of dual-channel imaging Aim 3: Validate the systemic performance with broad investigations of single cells and clinical applications