Wenhao Liu
BME PhD Proposal Presentation

Date:2022-02-03
Time: 10:00 a.m. to 11:30 a.m.
Location / Meeting Link: https://us02web.zoom.us/j/83776855057?pwd=Q21rZVFxYkkvQ3NKbFhERW9HM2lhUT09

Committee Members:
Takayama, Shuichi Robles, Francisco Tsygankov, Denis V Nie, Shuyi Jia, Shu


Title: Fourier light field microscopy: design, optimization, and its applications.

Abstract: Volumetric observation of cellular dynamic processes in a functional cluster, such as organoid or organ in tiny animals, is crucial for understanding how cells work together to realize complex functionality. As one of the most powerful tools that support such a probe, fluorescent microscopy has achieved much progress in the past decade. Among various advanced imaging techniques recently proposed, Fourier light-field microscopy is one of the most balanced among imaging volume, spatial resolution, and acquisition speed, allowing rapid, scanning-free volumetric imaging of low phototoxicity. Here, the Ph.D. candidate proposes to develop a two-color Fourier light-field Microscopy system and a fast reconstruction algorithm, to achieve large-scale, volumetric, multicolor imaging at single cellular resolution and camera limited speed. Based on the previous achievement, the candidate set three goals to further optimized his system: 1. Establish a duo-color FLFM using its wavefront partitioning nature. 2. Accelerate the reconstruction speed of FLFM via iterative Richardson-Lucy deconvolution with an unmatched back projector. 3. Give biological demonstrations to the newly developed hardware and software. The candidate expects the optimized FLFM system to be a more powerful tool for interrogating spatiotemporal-challenging intracellular interaction and disclosing the mechanism that underlies the function of organs.