Schedule:
11:00–12:00 pm Seminar 
12:00 pm Lunch will be served
12:00–1:00 pm Step-right-up Q&A Table
Bring your questions and project ideas for the new Fluidigm Helios CyTOF Instrument 

Getting Started with Mass Cytometry (CyTOF®)
Presented by Azucena Gomez-Cabrero, Ph.D.
Field Application Scientist, Fluidigm Mass Cytometry 

High-dimensional single-cell analysis has become critical to basic and clinical research. In traditional flow cytometry, the spectral overlap between fluorophores requires complicated data compensation and limits the number of markers measured, thus creating speed, labor and reproducibility bottlenecks. Mass Cytometry overcomes these limitations by utilizing antibodies labelled with rare earth metals. 2019 has been the most active year yet in the mass cytometry community with over 200 publications to date. CyTOF® technology has also been adopted by multiple consortia and in more than 40 clinical trials.
The Helios™, a CyTOF® system permits the analysis of 37+ unique targets on a per-cell basis enabling unparalleled insight into the phenotype and function of cell populations from normal and diseased states in a single tube. Additionally, barcoding for Mass Cytometry allows combining up to 20 samples in one tube for increased efficiency and to eliminate sample to sample variation. 
In this seminar, we will describe the workflow for the Helios™ system, how to get started with your own mass cytometry experiments, and the resources that are available to you to ensure your success.

Quantitative Single Cell Dissection of Tumor Microenvironments with Mass Cytometry Analysis 
Presented by Deon B. Doxie, Ph.D.
Winship Cancer Institute, Emory University