Beyond Icosahedra: The Many Faces of Virus-like Particles Revealed by Cryo Electron Microscopy

Abstract:

Single particle analysis (SPA) of cryo electron microscopy (cryEM) data is a powerful tool for structural characterization of biomolecules on a nanoscale level. The perfect sample for cryoEM is a monodisperse homogeneous solution of a single molecule of interest, in a single conformation, however it is not always possible. We use modern SPA tools to characterize a highly heterogenous mixture of virus-like particles (VLPs), explore multiple symmetries formed by VLPs, and highlight “the good, the bad and the ugly” of data processing. 

 Additionally, I will share insights into our utilization of bacterial surface display and the advancement of PCR techniques for environmental monitoring, highlighting evolutionary biology's pivotal role in overcoming complex challenges in clinical laboratory science. Information about other activities at NYSBC will also be described, including subcellular proteomics, correlative light/electron microscopy, and how to apply to the National EM centers for free access and training.