Using Force to Probe Bipolar Activation of Integrins and Signaling at Cell Surfaces

Event Details
Contact

James Godard

Faculty host:  Cheng Zhu

Summaries

Summary Sentence: Evan Evans, PhD - Boston University

Full Summary: BIOE Seminar SeriesEvan Evans, PhD - Professor, Department of Physics, Boston University"Using Force to Probe Bipolar Activation of Integrins and Signaling at Cell Surfaces"

ABSTRACT
Using an ultrasensitive force probe and basic statistical principles, we have developed a novel method to assay both the kinetic rates at which adhesive ligands dissociate from cell receptors and the rates at which the receptors separate from the cytoskeleton in the course of single molecule pulling experiments. Using results from probe tests of ICAM-1 bonds to L 2 integrin on leukocytes, I will show that cell signaling not only regulates lifetimes and strengths of the outer-ligand adhesive bond but also lifetimes and strengths of cohesive integrin anchoring to the cell cytostructure. Whether triggered from outside or inside the cell, the results indicate that the mechanical linkage between integrin tail domains and the cytoskeleton is a key effector of integrin activation in cell signaling (e.g. analogous to the “mechanical transmission” in an automobile).

 

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Additional Information

In Campus Calendar
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Groups

Parker H. Petit Institute for Bioengineering and Bioscience (IBB)

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Categories
Seminar/Lecture/Colloquium
Keywords
BioE Seminar, evan evans, Georgia Tech, IBB, Using Force to Probe Bipolar Activation of Integrins and Signaling at Cell Surfaces
Status
  • Created By: Colly Mitchell
  • Workflow Status: Published
  • Created On: Mar 1, 2011 - 5:14am
  • Last Updated: Oct 7, 2016 - 9:54pm