PhD Proposal by Alejandro J. Da Silva Sanchez

Event Details
  • Date/Time:
    • Tuesday January 19, 2021
      3:00 pm - 5:00 pm
  • Location: Remote: Blue Jeans
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  • URL: Bluejeans
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Summaries

Summary Sentence: The impact of the metabolic state of a cell on nucleic acid therapeutics

Full Summary: No summary paragraph submitted.

BioE PhD Proposal

Alejandro J. Da Silva Sanchez 

January 19th, 2021, 3:00 PM 

Link: https://bluejeans.com/425098357 

 

Advisor:

James E. Dahlman, Ph.D. BME, Georgia Institute of Technology and Emory University 

 

Committee Members: 

 

Philip J. Santangelo, Ph.D. 

BME, Georgia Institute of Technology and Emory University 

 

Julie A. Champion, Ph.D. 

ChBE, Georgia Institute of Technology 

 

Mark P. Styczynski, Ph.D. 

ChBE, Georgia Institute of Technology 

 

MG Finn, Ph.D. 

Chemistry and Biochemistry, Georgia Institute of Technology

 

 

 

The impact of the metabolic state of a cell on nucleic acid therapeutics 

 

Nucleic acid therapies have advanced over the last decade with the FDA approval of the first siRNA drug in 2018 and the recent approval of COVID vaccines leveraging mRNA technology. While surface receptors and endocytosis genes have been shown to influence the effectiveness of RNA drug delivery with lipid nanoparticles (LNPs), the effect of the metabolic state of a cell upon therapies seeking to produce or silence proteins remains understudied. This project therefore aims to (i) understand whether metabolic perturbations to the mTOR pathway upon PIP3 extracellular administration affect LNP-mediated mRNA delivery, (ii) develop cell- and mouse-agnostic high throughput LNP screening systems for siRNA and DNA drugs that will allow scientists to perform mechanistic studies on functional delivery with genetic knockout mice, and (iii) leverage these platforms to study whether mice exhibiting different levels of activity across the mTOR signaling pathway are more or less receptive to different nucleic acid drugs. This work will constitute early steps toward two equally important goals: (a) exploiting natural differences in cell signaling to improve cell type–specific nanoparticle delivery and (b) understanding how different physiological states can lead to different delivery potencies of nucleic acid therapeutics. 

 

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Graduate Studies

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Phd proposal
Status
  • Created By: Tatianna Richardson
  • Workflow Status: Published
  • Created On: Jan 7, 2021 - 2:17pm
  • Last Updated: Jan 7, 2021 - 2:17pm