PhD Proposal by Karen Martin

Event Details
  • Date/Time:
    • Tuesday March 17, 2020
      2:30 pm - 4:00 pm
  • Location: IBB 1128
  • Phone:
  • URL: BlueJeans Link
  • Email:
  • Fee(s):
    N/A
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Summaries

Summary Sentence: Biomaterial-directed mesenchymal stem cell immunomodulation for enhanced bone repair outcomes

Full Summary: No summary paragraph submitted.

Karen Martin

BioE PhD Proposal Presentation

Date: Tuesday, March 17, 2020

Time: 2:30 pm

Location: IBB 1128

 

 

Advisor:                            

Andrés García, Ph.D.

Committee:

Edward Botchwey, Ph.D.

Nick Willett, Ph.D.

Levi Wood, Ph.D.

Esma Yolcu, Ph.D.

 

 

Biomaterial-directed mesenchymal stem cell immunomodulation for enhanced bone repair outcomes

The immunomodulatory and pro-regenerative functions of mesenchymal stem cells (MSC) make them an attractive cell source for use in regenerative medicine applications. However, clinical translation is hampered by poor control over MSC survival, localization, phenotype, and secretome upon transplantation in vivo, as well as an incomplete understanding of how therapeutically delivered MSC interact with the host immune system to promote positive wound healing outcomes. The objective of this project is to utilize hydrogel delivery vehicles to promote MSC survival and immunomodulation in vivo and to evaluate the immune responses to these hydrogel-MSC therapies in a bone repair environment. This will be achieved through two specific aims: (1) Integrin-specific hydrogels will be evaluated for their ability to promote MSC persistence and immunomodulatory functions upon injection in vivo in immunocompetent mouse models. As there are several known species differences between human and murine immune cells and MSC, biomaterial-directed MSC secretome production and immune cell interactions will be evaluated in both a murine model using murine MSC and a humanized mouse model using human MSC, allowing for direct comparisons between species. (2) The impact of hydrogel-delivered MSC on bone healing and immune cell recruitment and phenotype over time will be evaluated in a murine segmental bone defect model. Using a combination of mass cytometry and single cell RNA-sequencing, key immune cell subpopulations that drive positive biomaterial-delivered MSC-dependent bone repair outcomes will be identified. The results of this proposal will yield critical insights into MSC-immune cell interactions in vivo and identify a means of modulating these interactions through the use of biomaterial-based MSC delivery vehicles.

 

Meeting URL

https://bluejeans.com/326073745?src=join_info

 

Meeting ID

326 073 745

Additional Information

In Campus Calendar
No
Groups

Graduate Studies

Invited Audience
Faculty/Staff, Public, Graduate students, Undergraduate students
Categories
Other/Miscellaneous
Keywords
Phd proposal
Status
  • Created By: Tatianna Richardson
  • Workflow Status: Published
  • Created On: Mar 4, 2020 - 1:57pm
  • Last Updated: Mar 16, 2020 - 1:13pm