Phd Defense by Stephen N. Housley, PT, DPT

Event Details
  • Date/Time:
    • Monday March 9, 2020
      9:00 am - 11:00 am
  • Location: Applied Physiology Building Room 1253
  • Phone:
  • URL:
  • Email:
  • Fee(s):
    N/A
  • Extras:
Contact
No contact information submitted.
Summaries

Summary Sentence: Chemotherapy Induced Sensory Neuropathy Depends on Non-Linear Interactions with Cancer

Full Summary: No summary paragraph submitted.

 

In partial fulfillment of the requirements for the degree of 

 

Doctor of Philosophy in Applied Physiology

in the 

School of Biological Sciences

 

Stephen N. Housley, PT, DPT

 

will defend his dissertation

 

Chemotherapy Induced Sensory Neuropathy Depends on Non-Linear Interactions with Cancer

Monday, March 9th, 2020

9:00 AM

Applied Physiology Building

555 14th St NW

Room 1253

 

Thesis Advisor:

Dr. Tim C. Cope

School of Biological Sciences and Coulter Department of Biomedical Engineering

Georgia Institute of Technology

  

Committee Members:

Dr. John McDonald

School of Biological Sciences

Georgia Institute of Technology

 

Dr. T. Richard Nichols

School of Biological Sciences

Georgia Institute of Technology

 

Dr. Edward Balog

School of Biological Sciences

Georgia Institute of Technology

 

Dr. Peter Wenner

Emory University School of Medicine

Emory University 

 

Abstract

For the constellation of neurological disorders known as chemotherapy induced neuropathy, mechanistic understanding, and treatment remain deficient.  In project one, I leveraged a multi-scale experimental approach to provide the first evidence that chronic sensory neuropathy depends on non-linear interactions between cancer and chemotherapy.  Global transcriptional profiling of dorsal root ganglia revealed amplified differential expression, notably in regulators of neuronal excitability, metabolism and inflammatory responses, all of which were unpredictable from effects observed with either chemotherapy or cancer alone.  Systemic interactions between cancer and chemotherapy also determined the extent of deficits in sensory encoding in vivo and ion channel protein expression by single mechanosensory neurons, with the potassium ion channel Kv3.3 emerging as candidate mechanisms explaining sensory neuron dysfunction. The sufficiency of this novel molecular mechanism was tested in an in silico biophysical model of mechanosensory function. Finally, validated measures of sensorimotor behavior in awake behaving animals confirmed that dysfunction after chronic chemotherapy treatment is exacerbated by cancer. Notably, errors in precise fore-limb placement emerged as a novel behavioral deficit unpredicted by our previous study of chemotherapy alone. These original findings identify novel contributors to peripheral neuropathy, and emphasize the fundamental dependence of neuropathy on the systemic interaction between chemotherapy and cancer across multiple levels of biological control.

In project two, I extend study to multiple classes of mechanosensory neurons that are necessary for generating the information content (population code) needed for proprioception. I first tested the hypothesis that exacerbated neuronal dysfunction is conserved across multiple classes of mechanosensory neurons. Results revealed co-suppression of specific signaling parameters across all neuronal classes. To understand the consequences of corrupt population code, I employed a long-short-term memory neural network (LSTM), a deep-learning algorithm, to test how decoding of spatiotemporal features of movement are altered after chemotherapy treatment of cancer. Results indicate that spiking activity from the population of neurons in animals with cancer, treated by chemotherapy contain significantly less information about key features of movement including, e.g. timing, magnitudes, and velocity. I then modeled the central nervous systems (CNS) capacity to compensate for this information loss. Even under optimal learning conditions, the inability to fully restore predictive power suggests that the CNS would not be able to compensate and restore full function. Our results support our proposal that lasting deficits in mobility and perception experienced by cancer survivors can originate from sensory information that is corrupted and un-interpretable by CNS neurons or networks.

Collectively, I present the first evidence that chronic cancer neuropathy cannot be explained by the effects of chemotherapy alone but instead depend on non-linear interactions with cancer. This understanding is a prerequisite for designing future studies and for developing effective treatments or preventative measures.

 

 

 

 

Additional Information

In Campus Calendar
No
Groups

Graduate Studies

Invited Audience
Faculty/Staff, Public, Graduate students, Undergraduate students
Categories
Other/Miscellaneous
Keywords
Phd Defense
Status
  • Created By: Tatianna Richardson
  • Workflow Status: Published
  • Created On: Feb 24, 2020 - 12:13pm
  • Last Updated: Feb 24, 2020 - 12:13pm