Micro-physiological Systems Seminar Series

Event Details
  • Date/Time:
    • Wednesday October 16, 2019
      11:00 am - 12:00 pm
  • Location: ES&T Building - Room L1118
  • Phone:
  • URL:
  • Email:
  • Fee(s):
    N/A
  • Extras:
Contact

Shuichi Takayama

Summaries

Summary Sentence: "Biomimetic Drugs: In Vitro, In Vivo & Mechanistic Studies of 5-12mer Antibacterial Peptoids to Treat Respiratory Infections" - Annelise E. Barron, Ph.D. - Stanford School of Medicine & Engineering

Full Summary: No summary paragraph submitted.

"Biomimetic Drugs: In Vitro, In Vivo & Mechanistic Studies of 5-12mer Antibacterial Peptoids to Treat Respiratory Infections"

Annelise E. Barron, Ph.D.
Associate Professor of Bioengineering
Stanford School of Medicine & School of Engineering

Growing bacterial resistance to conventional antibiotics has spurred the exploration of bioengineered antimicrobial peptides (AMPs) and mimetics as novel anti-infective agents. However, since peptide bioavailability is limited by proteolysis, non-natural AMP mimics are interesting as more robust and biostable analogues of AMP and seem to offer distinct advantages as potential clinical therapeutics. We report here on our experimental exploration of the development of poly-N-substituted glycines (peptoids) as a new class of biomimetic antimicrobial drugs, via multiple approaches including several different in vitro assays and in vivo mouse studies. After studying more than 120 peptoid sequence variants, we identified a number of unique peptoids that exhibit potent, broad-spectrum antibacterial in vitro activity, and which have a unique, biomimetic mechanism of action: bacterial rigidification. In our recent in vivo testing, mice were infected intratracheally with bioluminescent Pseudomonas aeruginosa, then treated by our TM5 peptoid, providing a significant reduction in bacterial loads compared to untreated animals. TM5 peptoid was also well tolerated in the lung by mice. In addition, new super-resolution fluorescence videomicroscopy studies confirm that these peptoids rapidly “rigidify” bacterial cytoplasm, just like natural cathelicidin AMPs. Taken together, these results show the highly promising potential clinical applicability of these 5-12mer peptoids.

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Additional Information

In Campus Calendar
Yes
Groups

Parker H. Petit Institute for Bioengineering and Bioscience (IBB)

Invited Audience
Faculty/Staff, Postdoc, Graduate students, Undergraduate students
Categories
Seminar/Lecture/Colloquium
Keywords
go-PetitInstitute, IBB
Status
  • Created By: Floyd Wood
  • Workflow Status: Published
  • Created On: Oct 7, 2019 - 12:10pm
  • Last Updated: Oct 7, 2019 - 12:23pm