PhD Proposal by Catherine Demos

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  • Date/Time:
    • Wednesday September 4, 2019
      1:00 pm - 3:00 pm
  • Location: HSRB E-182
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Summary Sentence: Role of novel flow sensitive transcription factor SIX2 in endothelial function and atherosclerosis

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Catherine Demos 

BME PhD Proposal Presentation

 

Date: September 4, 2019

Time: 1 pm

Location: HSRB E-182

 

Thesis Advisor: Hanjoong Jo, Ph.D.

Thesis Committee Members:

Changwon Park, Ph.D.

Wilbur Lam, MD, Ph.D.

James Dahlman, Ph.D.

Yuhong Fan, Ph.D.

 

Title: Role of novel flow sensitive transcription factor SIX2 in endothelial function and atherosclerosis

 

Abstract:

Atherosclerosis, a chronic inflammatory disease of the arteries and the underlying cause of heart attacks, ischemic strokes, and peripheral artery disease, preferentially occurs in curved and branching regions of the vasculature where endothelial cells experience low-magnitude and oscillatory shear stress from disturbed blood flow (d-flow). Atherosclerosis rarely occurs in straight, unbranched regions of the vasculature where endothelial cells experience high-magnitude, unidirectional shear stress from stable blood flow (s-flow). D-flow induces pro-atherogenic endothelial responses, while s-flow triggers atheroprotective changes driven by flow sensitive transcription factors (fTFs) in a process called endothelial reprogramming. Although there are many participating fTFs, the two most studied are KLF2 and KLF4 which are considered the master regulators of flow. However, KLF2/4 together account for less than 65% of flow sensitive gene changes therefore we hypothesize that there are additional master regulator fTFs which warrant investigation.

Through bioinformatics analysis, qPCR and immunostaining validation both in vivo and in vitro, we have identified the novel fTF SIX2 and propose that this transcription factor is an additional master regulator of flow. Early studies on SIX2 have focused on nephron progenitor cells and only recently has SIX2 been shown to have an effect in additional cell types. Reported functions of SIX2 include repressing cell reprogramming, maintaining stem-like qualities, and preventing epithelial-to-mesenchymal transition. This is a highly similar pathway to endothelial-to-mesenchymal transition (EndMT), an endothelial function which has long been studied as a driver of atherosclerotic progression. We have found SIX2 to be upregulated by s-flow and lost in d-flow as early as 12 hours post-surgery in our acute model of induced d-flow, indicating it is an early responder to shear stress and potentially plays a key role in preventing endothelial inflammation. Endothelial inflammation is also one of the initial changes observed under d-flow, and a major factor in atherosclerosis.

Taking these data together, we hypothesize that SIX2 is a master regulator of endothelial response to flow and a key factor in preventing atherosclerotic endothelial reprogramming, especially EndMT and inflammation. We will investigate this hypothesis in three aims: 1) determine the role of SIX2 in flow-dependent endothelial reprogramming, 2) identify flow-sensitive target genes of SIX2 and determine the mechanisms by which SIX2 regulates flow-dependent endothelial reprogramming, and 3) determine the effect of SIX2-deficiency in atherosclerosis in vivo. These studies will reveal the novel role of SIX2 in flow-dependent regulation of its target genes, endothelial function, and atherosclerosis. Our work may also reveal potential therapeutic targets of atherosclerosis.

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Phd proposal
Status
  • Created By: Tatianna Richardson
  • Workflow Status: Published
  • Created On: Aug 22, 2019 - 9:21am
  • Last Updated: Aug 22, 2019 - 9:21am