PhD Proposal by Nate Dwarshuis

Event Details
  • Date/Time:
    • Tuesday May 7, 2019
      12:00 pm - 2:00 pm
  • Location: EBB 5029
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Summaries

Summary Sentence: Optimizing T Cell Manufacturing and Quality Using Functionalized Microcarriers

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Nate Dwarshuis

BME PhD Proposal Presentation

 

Date: May 7th, 2019

Time: 12:00 PM

Location: EBB 5029

 

Advisor: Krishnendu Roy (Georgia Institute of Technology)

 

Committee Members:

Madhav Dhodapkar (Emory University)

Melissa Kemp (Georgia Institute of Technology)

Wilbur Lam (Georgia Institute of Technology)

Sakis Mantalaris (Georgia Institute of Technology)

 

Title: Optimizing T Cell Manufacturing and Quality Using Functionalized Microcarriers

 

Abstract/Summary:

 

Adoptive cell therapies (ACT) using chimeric antigen receptor (CAR) T cells have shown promise in treating cancer, but manufacturing large numbers of high quality cells remains challenging. Critically, current T cell expansion technologies only partially recapitulate the in vivo microenvironment found in the human lymph nodes. In these organs, T cells expand at high cell density with autocrine/paracrine signaling, as well as signals from the extracellular matrix (ECM). Here we propose a T cell expansion system using degradable gelatin microcarriers functionalized with anti-CD3 and anti-CD28 monoclonal antibodies (mAbs), which address several of these shortcomings by providing a 3D surface on which T cells can cluster at high cell density and remain in contact with ECM components.

 

The overall objective of this proposal will be to develop this microcarrier platform into a controllable process that can conceivably be used in industry. We will do this in three aims: 1) Develop a novel microcarrier platform and assess its performance relative to current state-of-the-art T cell expansion technology, 2) optimize the microcarrier system for in vivo performance using a design of experiments (DOE) approach, and 3) characterize microcarrier- expanded T cell of differing performance using multiomics techniques to generate predictive models. The result of these three aims will be a platform for expanding T cells and an accompanying set of mathematical models that can be used to predict and control process outcomes.

 

 

Additional Information

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Graduate Studies

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Faculty/Staff, Public, Graduate students, Undergraduate students
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Keywords
Phd proposal
Status
  • Created By: Tatianna Richardson
  • Workflow Status: Published
  • Created On: Apr 25, 2019 - 12:29pm
  • Last Updated: Apr 25, 2019 - 12:29pm