Petit Institute Seminar

Event Details
  • Date/Time:
    • Thursday April 7, 2016
      4:00 pm - 5:00 pm
  • Location: EBB Building, Room 5029
  • Phone:
  • URL:
  • Email:
  • Fee(s):
    N/A
  • Extras:
Contact

Bettina Bommarius

Summaries

Summary Sentence: "Label-Free & Dye-free Characterization of Protein Stability and Aggregation using nanoDSF" - Wyatt Strutz - NanoTemper Technologies Inc.

Full Summary: No summary paragraph submitted.

"Label-Free & Dye-free Characterization of Protein Stability and Aggregation using nanoDSF" 

Wyatt Strutz
Senior Application Scientist
NanoTemper Technologies Inc.

We will introduce the Prometheus NT.48 instrument and its novel nanoDSF technology, which allows for parallel high-precision characterization of stability and aggregation parameters of biologicals. nanoDSF is an advanced Differential Scanning Fluorimetry technology. It detects smallest changes in the fluorescence of tryptophan present in virtually all proteins.

The fluorescence of tryptophans in a protein is strongly dependent on its close surroundings. By following changes in fluorescence, chemical and thermal stability can be assessed in a truly label-free fashion. The dual-UV technology by NanoTemper allows for rapid fluorescence detection, providing an unmatched scanning speed and data point density. This yields an ultra-high resolution unfolding curves which allow for detection of even minute unfolding signals. Furthermore, since no secondary reporter fluorophores are required as in conventional DSF, protein solutions can be analyzed independent of buffer compositions, and over a concentration range of 200 mg/ml down to 5 μg/ml. Therefore, nanoDSF is the method of choice for easy, rapid and accurate analysis of protein folding and stability, with applications in membrane protein research, protein engineering, formulation development and quality control.

Additional Information

In Campus Calendar
Yes
Groups

Parker H. Petit Institute for Bioengineering and Bioscience (IBB)

Invited Audience
Undergraduate students, Faculty/Staff, Graduate students
Categories
Training/Workshop
Keywords
IBB
Status
  • Created By: Floyd Wood
  • Workflow Status: Published
  • Created On: Mar 23, 2016 - 6:58am
  • Last Updated: Apr 13, 2017 - 5:16pm