Emory + Children's Flow Cytometry Facility Seminar

Event Details
  • Date/Time:
    • Thursday August 15, 2013
      12:00 pm - 1:00 pm
  • Location: Main Auditorium, Health Research Sciences Bldg, 1760 Haygood Drive
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  • Fee(s):
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Contact

Shantisa Fulgham

Summaries

Summary Sentence: "Next Gen Cell-Sorting for Next Gen Sequence Detection Analysis" - Alice X. Wang - BD Biosciences

Full Summary: Emory + Children's Flow Cytometry Facility Seminar - "Next Gen Cell-Sorting for Next Gen Sequence Detection Analysis" - Alice X. Wang - BD Biosciences

"Next Gen Cell-Sorting for Next Gen Sequence Detection Analysis"

Alice X. Wang
BD Biosciences
San Jose, CA

The seminar will present how the power of flow cytometry can be exploited to isolate, characterize, phenotype and purify single cells from multiple cell populations prior to gene expression profiling. We describe a complete workflow starting with heterogeneous cancer cells that can be isolated at rapid speed by single cell depositions into 96 well plates using the newly developed flow cytometer from BD Biosciences, the BD FACSJazz™. We demonstrate the use of new analytical tools such as index cell sorting that can help track those individual cells during and after sorting. Finally we demonstrate high fidelity of the sorted cells using single cell gene expression profiling on the NanoString nCounter™ platform.

Lunch will be provided 11:30am-12:00pm in the HSRB Cafeteria.
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Parker H. Petit Institute for Bioengineering and Bioscience (IBB)

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Keywords
Alice X Wang
Status
  • Created By: Colly Mitchell
  • Workflow Status: Published
  • Created On: Aug 13, 2013 - 6:12am
  • Last Updated: Oct 7, 2016 - 10:04pm