{"73161":{"#nid":"73161","#data":{"type":"event","title":"Barbara A. Nsiah - Ph.D. Defense","body":[{"value":"\u003Cp\u003E\u003Cstrong\u003ECommittee Members\u003C\/strong\u003E\u003Cbr \/\u003ETodd C. McDevitt, Ph.D. (advisor)\u003Cbr \/\u003ERobert M. Nerem, Ph.D. (advisor)\u003Cbr \/\u003EEvan A. Zamir, Ph.D.\u003Cbr \/\u003EManu O. Platt, Ph.D.\u003Cbr \/\u003ERudolph L. Gleason, Ph.D.\u003Cbr \/\u003E\u003Cbr \/\u003E\u003Cbr \/\u003E\u0026nbsp;\u0026nbsp;\u0026nbsp;\u0026nbsp;\u0026nbsp;\u0026nbsp;\u0026nbsp; Vascularization of tissue-engineered substitutes is imperative for successful implantation into sites of injury.\u0026nbsp; Strategies to promote vascularization within tissue-engineered constructs have focused on incorporating endothelial or endothelial progenitor cells within the construct.\u0026nbsp; However, since endothelial (ECs) and endothelial progenitor cells are adult cell types and limited in number, acquiring quantities needed for regenerative medicine applications is not feasible.\u0026nbsp; Pluriopotent stem cells have been explored as a cell source for tissue-engineered substitutes because of their inherent ability to differentiate into all somatic cell types, including endothelial cells (ECs). \u0026nbsp;\u003Cbr \/\u003E\u0026nbsp;\u0026nbsp;\u0026nbsp;\u0026nbsp;\u0026nbsp;\u0026nbsp;\u0026nbsp; Current EC differentiation strategies require laborious and extensive culture periods, utilize large quantities of expensive growth factors and extracellular matrix, and generally yield heterogenous populations for which only a small percentage of the differentiated cells are ECs.\u0026nbsp; In order to recapitulate in vivo embryonic stem cell (ESC) differentiation, 3D stem cell aggregates or embryoid bodies (EBs) have been employed in vitro.\u0026nbsp; In the developing embryo, fluid shear stress, VEGF, and oxygen are instructive cues for endothelial differentiation and vasculogenesis.\u0026nbsp; Thus, the objective of this work was to study the effects of fluid shear stress pre-conditioning of ESCs on EB endothelial differentiation and vasculogensis.\u0026nbsp; Our overall hypothesis is that exposing ESCs to fluid shear stress prior to EB differentiation will promote EB endothelial differentiation and vasculogenesis.\u003Cbr \/\u003E\u0026nbsp;\u0026nbsp; \u0026nbsp;Pre-conditioning ESCs with fluid shear stress modulated EB differentiation as well as endothelial cell-like cellular organization and EB morphogenesis.\u0026nbsp; To further promote endothelial differentiation, ESCs pre-conditioned with shear were treated with VEGF.\u0026nbsp; Exposing EBs formed from ESCs pre-conditioned with shear to low oxygen resulted in increased production of VEGF and formation of endothelial networks.\u0026nbsp; The results of this work demonstrate the role that physical forces play in modulating stem cell fate and morphogenesis.\u003C\/p\u003E","summary":null,"format":"limited_html"}],"field_subtitle":"","field_summary":[{"value":"\u003Cp\u003EVascularization of tissue-engineered substitutes is imperative for successful implantation into sites of injury.\u003C\/p\u003E","format":"limited_html"}],"field_summary_sentence":[{"value":"\u0022Fluid Shear Stress Modulation of Embryonic Stem Cell Differentiation\u0022"}],"uid":"27547","created_gmt":"2011-11-29 16:47:50","changed_gmt":"2016-10-08 01:56:45","author":"Chris Ruffin","boilerplate_text":"","field_publication":"","field_article_url":"","field_event_time":{"event_time_start":"2011-12-07T10:30:00-05:00","event_time_end":"2011-12-07T12:30:00-05:00","event_time_end_last":"2011-12-07T12:30:00-05:00","gmt_time_start":"2011-12-07 15:30:00","gmt_time_end":"2011-12-07 17:30:00","gmt_time_end_last":"2011-12-07 17:30:00","rrule":null,"timezone":"America\/New_York"},"extras":[],"groups":[{"id":"65448","name":"Bioengineering Graduate Program"}],"categories":[],"keywords":[{"id":"13785","name":"Barbara Nsiah"},{"id":"569","name":"bioengineering"}],"core_research_areas":[],"news_room_topics":[],"event_categories":[{"id":"1788","name":"Other\/Miscellaneous"}],"invited_audience":[],"affiliations":[],"classification":[],"areas_of_expertise":[],"news_and_recent_appearances":[],"phone":[],"contact":[{"value":"\u003Cp\u003E\u003Ca href=\u0022mailto:chris.ruffin@ibb.gatech.edu\u0022\u003EChristopher Ruffin\u003C\/a\u003E\u003C\/p\u003E","format":"limited_html"}],"email":[],"slides":[],"orientation":[],"userdata":""}}}