{"72866":{"#nid":"72866","#data":{"type":"event","title":"Breakfast Club Seminar Series","body":[{"value":"\u003Cp\u003E\u003Cstrong\u003E\u003Cem\u003E\u0022\u003Cstrong\u003EThe Two Unknowns of Nucleosomes: How they are Formed and How they are Removed\u003C\/strong\u003E\u0022\u003C\/em\u003E\u003Cbr \/\u003E\u003Cbr \/\u003EHarold Kim, PhD - Assistant Professor, School of Physics\u003Cbr \/\u003E\u003C\/strong\u003E\u003C\/p\u003E\u003Cp\u003EIn eukaryotes, the genomic DNA is highly packaged inside the\nnucleus of a cell by forming beads-on-a string-like structure called\nnucleosomes. The propensity of a ~150-bp duplex DNA to form a nucleosome,\ntermed the \u0022nucleosome affinity\u0022, varies over a few orders of\nmagnitude depending on the DNA sequence. Although nucleosome affinity is thought\nto be determined by DNA bending stiffness, the exact relationship between the\ntwo is not clear. Besides their role in DNA packaging, nucleosomes can repress\ngene expression by preventing transcription factors from accessing their DNA\nbinding sites. Some nucleosomes, however, do not directly occlude transcription\nfactor binding sites, and therefore their role in gene expression remains\nunknown. In the first part of this talk, I will explain how we measure looping\nkinetics and permanent curvature of various sequences of DNA using\nsingle-molecule FRET (fluorescence resonance energy transfer) and gel\nelectrophoresis. I will then estimate nucleosome affinity of different sequences\nbased on these measurements. In the second part, I will explain how we quantify\ngene expression pattern using fluorescence microscopy of yeast cells and demonstrate\ntwo examples where a nucleosome exhibits opposite effects on gene expression.\nBased on these results, I will present a model for nucleosome removal prior to\ntranscription initiation.\u003C\/p\u003E\u003Cp\u003E\u003Cbr \/\u003EThe IBB Breakfast Club seminar series was started with the spirit of the Institute\u0027s interdisciplinary mission in mind. The goal of the seminar series is to highlight research taking place throughout the institute to enable the IBB community to further collaborative opportunities and interdisciplinary research. Faculty are often asked to speak at other universities and conferences, but rarely present at their home institution, this seminar series is an attempt to close that gap. The IBB Breakfast Club is open to anyone in the bio-community.\u0026nbsp;\u003C\/p\u003E","summary":null,"format":"limited_html"}],"field_subtitle":"","field_summary":[{"value":"\u003Cp\u003EBreakfast Club Seminar Series - \u0022The\ntwo unknowns of nucleosomes: how they are formed and how they are removed\u0022 -\u0026nbsp;Harold Kim, PhD - Assistant Professor, School of Physics\u003C\/p\u003E","format":"limited_html"}],"field_summary_sentence":[{"value":"\u0022The two unknowns of nucleosomes: how they are formed and how they are removed\u0022 - Harold Kim, PhD - Assistant Professor, School of Physics"}],"uid":"27195","created_gmt":"2011-11-17 11:53:45","changed_gmt":"2016-10-08 01:56:41","author":"Colly Mitchell","boilerplate_text":"","field_publication":"","field_article_url":"","field_event_time":{"event_time_start":"2012-03-13T09:30:00-04:00","event_time_end":"2012-03-13T10:30:00-04:00","event_time_end_last":"2012-03-13T10:30:00-04:00","gmt_time_start":"2012-03-13 13:30:00","gmt_time_end":"2012-03-13 14:30:00","gmt_time_end_last":"2012-03-13 14:30:00","rrule":null,"timezone":"America\/New_York"},"extras":[],"hg_media":{"72878":{"id":"72878","type":"image","title":"Harold Kim, PhD - Assistant Professor, School of Physics","body":null,"created":"1449177962","gmt_created":"2015-12-03 21:26:02","changed":"1475894665","gmt_changed":"2016-10-08 02:44:25","alt":"Harold Kim, PhD - Assistant Professor, School of Physics","file":{"fid":"193713","name":"harold_kim.jpg","image_path":"\/sites\/default\/files\/images\/harold_kim_0.jpg","image_full_path":"http:\/\/hg.gatech.edu\/\/sites\/default\/files\/images\/harold_kim_0.jpg","mime":"image\/jpeg","size":25077,"path_740":"http:\/\/hg.gatech.edu\/sites\/default\/files\/styles\/740xx_scale\/public\/images\/harold_kim_0.jpg?itok=99-YQzbX"}},"70917":{"id":"70917","type":"image","title":"Breakfast Club Seminar Series","body":null,"created":"1449177328","gmt_created":"2015-12-03 21:15:28","changed":"1475894625","gmt_changed":"2016-10-08 02:43:45","alt":"Breakfast Club Seminar Series","file":{"fid":"193471","name":"logobldg_graphic.png","image_path":"\/sites\/default\/files\/images\/logobldg_graphic_0.png","image_full_path":"http:\/\/hg.gatech.edu\/\/sites\/default\/files\/images\/logobldg_graphic_0.png","mime":"image\/png","size":241588,"path_740":"http:\/\/hg.gatech.edu\/sites\/default\/files\/styles\/740xx_scale\/public\/images\/logobldg_graphic_0.png?itok=34gqHmzX"}}},"media_ids":["72878","70917"],"related_links":[{"url":"http:\/\/sites.google.com\/site\/haroldkimlab\/","title":"Kim lab"},{"url":"http:\/\/www.ibb.gatech.edu\/breakfast-club","title":"IBB Breakfast Club"}],"groups":[{"id":"1292","name":"Parker H. Petit Institute for Bioengineering and Bioscience (IBB)"}],"categories":[],"keywords":[{"id":"11879","name":"BK Club"},{"id":"15109","name":"harold kim"},{"id":"248","name":"IBB"},{"id":"25791","name":"The Two Unknowns of Nucleosomes: How they are Formed and How they are Removed"}],"core_research_areas":[],"news_room_topics":[],"event_categories":[{"id":"1795","name":"Seminar\/Lecture\/Colloquium"}],"invited_audience":[],"affiliations":[],"classification":[],"areas_of_expertise":[],"news_and_recent_appearances":[],"phone":[],"contact":[{"value":"\u003Cp\u003E\u003Ca href=\u0022mailto:colly.mitchell@ibb.gatech.edu\u0022\u003EColly Mitchell\u003C\/a\u003E\u003C\/p\u003E","format":"limited_html"}],"email":[],"slides":[],"orientation":[],"userdata":""}}}