{"72469":{"#nid":"72469","#data":{"type":"event","title":"Ted T. Lee - Ph.D. Proposal","body":[{"value":"\u003Cp\u003E\u003Cstrong\u003EAdvisor\u003C\/strong\u003E:\u0026nbsp; Andr\u00e9s J. Garc\u00eda, Ph.D.\u0026nbsp; (Georgia Institute of Technology)\u003Cbr \/\u003E\u003Cstrong\u003ECommittee\u003C\/strong\u003E:\u003Cbr \/\u003EAr\u00e1nzazu del Campo, Ph.D.\u0026nbsp; (Max-Planck-Institut f\u00fcr Polymerforschung)\u003Cbr \/\u003ETom Barker, Ph.D. (Georgia Institute of Technology)\u003Cbr \/\u003EEvan Zamir, Ph.D. (Georgia Institute of Technology)\u003Cbr \/\u003ECheng Zhu, Ph.D. (Georgia Institute of Technology)\u003Cbr \/\u003E\u003Cbr \/\u003ECells rely on time-dependent binding and activation by the ECM to initiate downstream signal transduction.\u0026nbsp; It is unknown whether adhesion to a ligand is required throughout various cell processes, or only during a specified time period (\u201ctemporal threshold\u201d). Current approaches to ligand presentation often comprise of static, constant densities of ligands.\u0026nbsp; In contrast, natural cell adhesive interactions with ECMs exhibit spatiotemporal patterns of binding and activation.\u0026nbsp; Therefore, a key to future research in controlling cell-material interactions will be the development of materials that can respond to external stimuli. \u0026nbsp;\u003Cbr \/\u003E\u003Cbr \/\u003EThe objective of this project is to engineer biomaterials that present a UV-labile caged-Arginine-Glycine-Aspartic Acid (RGD) peptide and evaluate its effects on cell activities.\u0026nbsp;\u0026nbsp; RGD is the minimal adhesive sequence of fibronectin.\u0026nbsp; In this caged-peptide, a photo-labile group adjacent to the aspartic acid residue of RGD effectively \u201cmasks\u201d a cyclo(RGDfk) peptide.\u0026nbsp; Upon UV irradiation (360 nm), the caging group is released thereby restoring the adhesive activity of the peptide.\u0026nbsp; We have successfully tethered this caged-RGD to both poly(ethylene glycol)-based hydrogels and PDMS surfaces.\u0026nbsp; Using these systems, we will be able to dynamically modulate adhesive ligand presentation to elucidate the effects of temporal control on various cell processes.\u003C\/p\u003E","summary":null,"format":"limited_html"}],"field_subtitle":"","field_summary":[{"value":"\u003Cp\u003EDynamic Ligand Presentation of Caged-RGD\u003C\/p\u003E","format":"limited_html"}],"field_summary_sentence":[{"value":"\u0022Dynamic Ligand Presentation of Caged-RGD\u0022"}],"uid":"27547","created_gmt":"2011-11-08 11:25:55","changed_gmt":"2016-10-08 01:56:37","author":"Chris Ruffin","boilerplate_text":"","field_publication":"","field_article_url":"","field_event_time":{"event_time_start":"2011-11-16T08:00:00-05:00","event_time_end":"2011-11-16T11:00:00-05:00","event_time_end_last":"2011-11-16T11:00:00-05:00","gmt_time_start":"2011-11-16 13:00:00","gmt_time_end":"2011-11-16 16:00:00","gmt_time_end_last":"2011-11-16 16:00:00","rrule":null,"timezone":"America\/New_York"},"extras":[],"groups":[{"id":"65448","name":"Bioengineering Graduate Program"}],"categories":[],"keywords":[{"id":"569","name":"bioengineering"},{"id":"15005","name":"Ted Lee"}],"core_research_areas":[],"news_room_topics":[],"event_categories":[{"id":"1788","name":"Other\/Miscellaneous"}],"invited_audience":[],"affiliations":[],"classification":[],"areas_of_expertise":[],"news_and_recent_appearances":[],"phone":[],"contact":[{"value":"\u003Cp\u003E\u003Ca href=\u0022mailto:chris.ruffin@ibb.gatech.edu\u0022\u003EChris Ruffin\u003C\/a\u003E\u003C\/p\u003E","format":"limited_html"}],"email":[],"slides":[],"orientation":[],"userdata":""}}}