{"70505":{"#nid":"70505","#data":{"type":"event","title":"Biological Networks Follow the Flow!","body":[{"value":"\u003Cp\u003ERob Krams\u003Cbr \/\u003E\nChair in Molecular Bioengineering\u003Cbr \/\u003E\nDepartment of Bioengineering\u003Cbr \/\u003E\nImperial College London\u003Cbr \/\u003E\nUnited Kingdom\u003Cbr \/\u003E\nthursday, august 18th\u003Cbr \/\u003E\n11:00 a.m.\u003Cbr \/\u003E\nroom 1128 \u2022 ibb\u003Cbr \/\u003E\nAtherosclerotic plaques are non-uniformly distributed over the arterial system and ample evidence point to a role of blood flow in the formation of these atherosclerotic predilection sites. Blood flow exerts its effect on the vessel wall through mechanical activation of ~ 7 endothelial\u003Cbr \/\u003E\nsignalling pathways and 8 acknowledged transcription factors, controlling ~ 2000 endothelial genes. As a consequence, endothelial cells express adhesion factors, change their permeability to lipids and produce diffusible factors under the influence of blood flow which affect the signalling in and composition of the vascular wall.\u003Cbr \/\u003E\nIn our group, signalling networks in the endothelial cell are studied with commercially available(microarrays\/rapid sequencer) and custom-designed high throughput analysis methods (siRNA platform; top-down method) and by studying small signalling modules (bottom-up method). The screens from microarrays and rapid sequencers are analysed with methods to infer signalling networks and a new siRNA platform is developed for a systematic analysis of these networks to rapidly and systematically validate these networks. In parallel to this approach, small signalling\u003Cbr \/\u003E\nmodules are simulated and experimentally tested in flow chambers with the aim to build larger\u003Cbr \/\u003E\nnetworks. We will discuss two signalling networks: the IKK-NF-\u03baB pathway and the MAPK5-KLF-pathways in relation to their regulation of the eNOS-NO pathway.\u003Cbr \/\u003E\nBlood flow also exerts effect on plaque growth and plaque composition with a currently unknownmechanism. We will provide data for a cellular-chemokine biological network induced by low shear stress that plays in important role in vulnerable plaque formation. We will present ongoing studies aimed at extending this idea and using it as a tool for molecular imaging.\u003Cbr \/\u003E\nFinally, we aim to bring these two lines of research together where the mechaonsensitive signalling pathways of endothelial cells are studied and targeted for prevention of plaque formation.\u003C\/p\u003E","summary":null,"format":"limited_html"}],"field_subtitle":"","field_summary":[{"value":"Dr. Krans will discuss his research on the molecular mechanism underlying biomechanical stimuli. To that end he uses a combination of engineering techniques (imaging, systems biology and synthetic biology) and molecular techniques (high throughput, qPCR,","format":"limited_html"}],"field_summary_sentence":[{"value":"BME Guest Presentation - Rob Krams, Imperial College London"}],"uid":"27182","created_gmt":"2011-08-01 00:00:00","changed_gmt":"2016-10-08 01:55:54","author":"Adrianne Proeller","boilerplate_text":"","field_publication":"","field_article_url":"","field_event_time":{"event_time_start":"2011-08-01T12:00:00-04:00","event_time_end":"2011-08-19T13:00:00-04:00","event_time_end_last":"2011-08-19T13:00:00-04:00","gmt_time_start":"2011-08-01 16:00:00","gmt_time_end":"2011-08-19 17:00:00","gmt_time_end_last":"2011-08-19 17:00:00","rrule":null,"timezone":"America\/New_York"},"extras":[],"related_links":[{"url":"http:\/\/www3.imperial.ac.uk\/people\/r.krams","title":"Rob Krams"}],"groups":[{"id":"1254","name":"Wallace H. Coulter Dept. of Biomedical Engineering"}],"categories":[],"keywords":[{"id":"7270","name":"atherosclerosis"},{"id":"569","name":"bioengineering"},{"id":"2548","name":"biomedical"},{"id":"1440","name":"blood"},{"id":"7104","name":"cardiovascular"}],"core_research_areas":[],"news_room_topics":[],"event_categories":[{"id":"1795","name":"Seminar\/Lecture\/Colloquium"}],"invited_audience":[],"affiliations":[],"classification":[],"areas_of_expertise":[],"news_and_recent_appearances":[],"phone":[],"contact":[{"value":"\u003Cstrong\u003EAdrianne Proeller\u003C\/strong\u003E\u003Cbr \/\u003EWallace H. Coulter Dept. of Biomedical Engineering\u003Cbr \/\u003E\u003Ca href=\u0022http:\/\/www.gatech.edu\/contact\/index.html?id=aproeller3\u0022\u003EContact Adrianne Proeller\u003C\/a\u003E","format":"limited_html"}],"email":[],"slides":[],"orientation":[],"userdata":""}}}