{"685810":{"#nid":"685810","#data":{"type":"event","title":"PhD Defense by Justin Hosten","body":[{"value":"\u003Cp\u003EJustin Hosten\u003Cbr\u003EBME PhD\u0026nbsp;Defense\u0026nbsp;Presentation\u003Cbr\u003E\u003Cbr\u003E\u003Cstrong\u003EDate\u003C\/strong\u003E: 2025-10-17\u003Cbr\u003E\u003Cstrong\u003ETime\u003C\/strong\u003E: 2:00pm\u003C\/p\u003E\u003Cp\u003E\u003Cstrong\u003ELocation \/ Meeting Link\u003C\/strong\u003E: HSRBII, N600 [\u003Ca href=\u0022https:\/\/emory.zoom.us\/j\/98452979800\u0022 target=\u0022_blank\u0022 title=\u0022https:\/\/emory.zoom.us\/j\/98452979800\u0022\u003Ehttps:\/\/emory.zoom.us\/j\/98452979800\u003C\/a\u003E]\u0026nbsp;\u003C\/p\u003E\u003Cp\u003E\u0026nbsp;\u0026nbsp;\u0026nbsp;\u0026nbsp;\u0026nbsp;\u0026nbsp;\u0026nbsp;\u0026nbsp;\u0026nbsp;\u0026nbsp;\u0026nbsp;\u0026nbsp;\u0026nbsp;\u0026nbsp;\u0026nbsp;\u0026nbsp;\u0026nbsp;\u0026nbsp;\u0026nbsp;\u0026nbsp;\u0026nbsp;\u0026nbsp;\u0026nbsp;\u0026nbsp;\u0026nbsp;\u0026nbsp;\u0026nbsp;\u0026nbsp;\u0026nbsp;\u0026nbsp;\u0026nbsp;\u0026nbsp;\u0026nbsp;\u0026nbsp;\u0026nbsp;\u0026nbsp;\u0026nbsp;\u0026nbsp;\u0026nbsp;\u0026nbsp;\u0026nbsp;\u0026nbsp;\u0026nbsp;\u0026nbsp;\u0026nbsp;\u0026nbsp;\u0026nbsp;\u0026nbsp;\u0026nbsp;\u0026nbsp;\u0026nbsp;\u0026nbsp;\u0026nbsp;\u0026nbsp;\u0026nbsp;\u0026nbsp;\u0026nbsp;\u0026nbsp;\u0026nbsp;\u0026nbsp;\u0026nbsp;\u0026nbsp;\u0026nbsp;\u0026nbsp;\u0026nbsp;\u0026nbsp;\u0026nbsp;\u0026nbsp;\u0026nbsp;\u0026nbsp;\u0026nbsp;\u0026nbsp;\u0026nbsp;\u0026nbsp;\u0026nbsp;\u0026nbsp;\u0026nbsp;\u0026nbsp;\u0026nbsp;\u0026nbsp;\u0026nbsp;\u0026nbsp;\u0026nbsp;\u0026nbsp;\u0026nbsp;\u0026nbsp;\u0026nbsp;\u0026nbsp;\u0026nbsp;\u0026nbsp;\u0026nbsp;\u0026nbsp;\u0026nbsp;\u0026nbsp;\u0026nbsp;\u0026nbsp;\u0026nbsp;\u0026nbsp;\u0026nbsp;\u0026nbsp;\u0026nbsp;\u003Cbr\u003E\u003Cstrong\u003ECommittee Members:\u003C\/strong\u003E\u003Cbr\u003ERabin Tirouvanziam, PhD (Co-Advisor); Krish Roy, PhD (Co-Advisor); Karmella Haynes, PhD; Jamal Lewis, PhD; Shuichi Takayama, PhD\u003Cbr\u003E\u003Cbr\u003E\u003Cbr\u003E\u003Cstrong\u003ETitle\u003C\/strong\u003E: Biomimetic model of human small airway monocyte and macrophage recruitment, adaptation, and function\u003Cbr\u003E\u003Cbr\u003E\u003Cstrong\u003EAbstract:\u003C\/strong\u003E\u003Cbr\u003ESeveral muco-obstructive lung diseases in humans, including cystic fibrosis (CF), feature chronic recruitment of blood leukocytes and their reprogramming within the small-airway lumen, driving pathological adaptations such as dysregulated immune-mediator secretion and impaired bacterial killing. \u0026nbsp;Most current studies focus on neutrophils or rely on in vivo\/in vitro models that do not capture the nuances of the small airway. \u0026nbsp;To investigate these mechanisms, we engineered a biomimetic small-airway model in which primary human blood monocytes transmigrate across a human small-airway epithelium and, after 4-day exposure to macrophage colony-stimulating factor (M-CSF), differentiate into macrophages.\u0026nbsp; Airway-recruited monocytes (ArMos), generated using the control chemoattractant CCL2, underwent phenotypic, transcriptomic, and secretomic reprogramming upon transmigration and further changes upon differentiation into airway-recruited macrophages (ArMas), relative to circulating monocytes. \u0026nbsp;Compared with non-transmigrated, M-CSF\u2013differentiated macrophages, both ArMos and ArMas retained airway-associated features that are otherwise lost ex vivo. \u0026nbsp;Conditioning with CF airway supernatant (cell- and bacteria-free sputum) produced additional, specific alterations in ArMos and ArMas across phenotype, transcriptome, and secretome compared with CCL2 conditions. \u0026nbsp;Critically, CF ArMas (but not CF ArMos) showed a markedly reduced ability to kill Pseudomonas aeruginosa, a common CF pathogen. \u0026nbsp;These findings extend prior work on small-airway\u2013recruited neutrophils and identify macrophage immune tolerance as one contributor to bacterial colonization in CF. \u0026nbsp;Our biomimetic model supports further mechanistic and preclinical investigations of small airway-recruited monocytes and macrophages in health and CF, as well as in other diseases featuring chronic airway inflammation and infection, such as non-CF bronchiectasis and chronic obstructive pulmonary disease (COPD).\u003C\/p\u003E\u003Cp\u003E\u0026nbsp;\u003C\/p\u003E\u003Cp\u003E\u0026nbsp;\u003C\/p\u003E","summary":"","format":"limited_html"}],"field_subtitle":"","field_summary":[{"value":"\u003Cp\u003EBiomimetic model of human small airway monocyte and macrophage recruitment, adaptation, and function\u003C\/p\u003E","format":"limited_html"}],"field_summary_sentence":[{"value":"Biomimetic model of human small airway monocyte and macrophage recruitment, adaptation, and function"}],"uid":"27707","created_gmt":"2025-10-20 12:27:12","changed_gmt":"2025-10-20 12:27:42","author":"Tatianna Richardson","boilerplate_text":"","field_publication":"","field_article_url":"","field_event_time":{"event_time_start":"2025-10-17T14:00:00-04:00","event_time_end":"2025-10-17T16:00:00-04:00","event_time_end_last":"2025-10-17T16:00:00-04:00","gmt_time_start":"2025-10-17 18:00:00","gmt_time_end":"2025-10-17 20:00:00","gmt_time_end_last":"2025-10-17 20:00:00","rrule":null,"timezone":"America\/New_York"},"location":"HSRBII, N600 ","extras":[],"groups":[{"id":"221981","name":"Graduate Studies"}],"categories":[],"keywords":[{"id":"100811","name":"Phd Defense"}],"core_research_areas":[],"news_room_topics":[],"event_categories":[{"id":"1788","name":"Other\/Miscellaneous"}],"invited_audience":[{"id":"78771","name":"Public"}],"affiliations":[],"classification":[],"areas_of_expertise":[],"news_and_recent_appearances":[],"phone":[],"contact":[],"email":[],"slides":[],"orientation":[],"userdata":""}}}