{"681665":{"#nid":"681665","#data":{"type":"event","title":"PhD Defense by Cara Khayat","body":[{"value":"\u003Cp\u003ECara Khayat\u003Cbr\u003EBME PhD Defense Presentation\u003Cbr\u003E\u003Cbr\u003E\u003Cstrong\u003EDate\u003C\/strong\u003E: 2025-04-15\u003Cbr\u003E\u003Cstrong\u003ETime\u003C\/strong\u003E: 10:30 am\u003Cbr\u003E\u003Cstrong\u003ELocation \/ Meeting Link\u003C\/strong\u003E: Suddath Seminar Room 1128; \u003Ca href=\u0022https:\/\/emory.zoom.us\/j\/97710303583\u0022\u003Ehttps:\/\/emory.zoom.us\/j\/97710303583\u003C\/a\u003E\u003Cbr\u003E\u003Cbr\u003E\u003Cstrong\u003ECommittee Members:\u003C\/strong\u003E\u003Cbr\u003ECheng Zhu; Levi Wood, Andres Garcia, Ross Ethier, Machelle Pardue, Stephen Traynelis\u003Cbr\u003E\u003Cbr\u003E\u003Cbr\u003E\u003Cstrong\u003ETitle\u003C\/strong\u003E: The Mechanobiology of the Glutamate Delta 1 Receptor\u003Cbr\u003E\u003Cbr\u003E\u003Cstrong\u003EAbstract:\u003C\/strong\u003E\u003Cbr\u003EThe glutamate delta 1 receptor (GluD1), despite sequence homology with related receptors, does not function in a classically ionotropic way. Instead, it bridges pre- and post-synaptic terminals by binding Neurexin 1\u03b2 (Nrxn1\u03b2) via Cerebellin 2 (Cbln2), influencing synaptogenesis. Genetic links between GluD1 are associated with neurodevelopmental and neuropsychiatric disorders, such as schizophrenia, autism, and bipolar disorder. In this work, we hypothesize that GluD1\u2013Cbln2\u2013Nrxn1\u03b2 binding in synaptogenesis is regulated by neuron-generated, cytoskeleton-driven forces at the synaptic cleft, and to support this GluD1 must be a mechanosensitive receptor which responds to force. To test this, we used advanced biophysical tools (Biomembrane Force Probe, DNA-based tension probes, and Tension Gauge Tethers) alongside traditional neurobiological techniques (immunohistochemistry, calcium dynamics) across three aims: 1) Generate protein\/cell systems to study force-dependent 2D binding and cell-generated forces on the Nrxn1\u03b2\u2013Cbln2\u2013GluD1 complex. 2) Investigate the effects of neuromodulators like GABA and D-serine, and clinically relevant mutants, on GluD1\u2019s mechanosensitivity. 3) Evaluate the role of these interactions in calcium signaling and synaptogenesis. In this work, we present the first affinity interactions of the tripartite GluD1\u2013Cbln2\u2013Nrxn1\u03b2, positive cooperativity that converts a slip bond to a catch bond in the tripartite binding, emphasizing the importance of force, and that cells generate endogenous pulling forces through the GluD1 receptor in the tripartite complex. Furthermore, we show that GluD1\u2019s mechanosensitivity is impacted by neuromodulators GABA and D-serine, and a clinically relevant mutant changes GluD1\u2019s binding properties and mechanosensitivity. Finally, we show that force in GluD1\u2019s binding also translates into differences in signaling through calcium and changes in differentiation patterns when force generation is restricted.\u0026nbsp;\u003C\/p\u003E\u003Cp\u003E\u0026nbsp;\u003C\/p\u003E","summary":"","format":"limited_html"}],"field_subtitle":"","field_summary":[{"value":"\u003Cp\u003ESuddath Seminar Room 1128;\u0026nbsp;\u003C\/p\u003E","format":"limited_html"}],"field_summary_sentence":[{"value":"Suddath Seminar Room 1128; "}],"uid":"27707","created_gmt":"2025-04-08 16:06:14","changed_gmt":"2025-04-08 16:06:14","author":"Tatianna Richardson","boilerplate_text":"","field_publication":"","field_article_url":"","field_event_time":{"event_time_start":"2025-04-15T10:30:00-04:00","event_time_end":"2025-04-15T12:30:00-04:00","event_time_end_last":"2025-04-15T12:30:00-04:00","gmt_time_start":"2025-04-15 14:30:00","gmt_time_end":"2025-04-15 16:30:00","gmt_time_end_last":"2025-04-15 16:30:00","rrule":null,"timezone":"America\/New_York"},"location":"Suddath Seminar Room 1128; ","extras":[],"groups":[{"id":"221981","name":"Graduate Studies"}],"categories":[],"keywords":[{"id":"100811","name":"Phd Defense"}],"core_research_areas":[],"news_room_topics":[],"event_categories":[{"id":"1788","name":"Other\/Miscellaneous"}],"invited_audience":[{"id":"78771","name":"Public"}],"affiliations":[],"classification":[],"areas_of_expertise":[],"news_and_recent_appearances":[],"phone":[],"contact":[],"email":[],"slides":[],"orientation":[],"userdata":""}}}