{"680589":{"#nid":"680589","#data":{"type":"event","title":"PhD Defense by Ling Zhu","body":[{"value":"\u003Cp\u003E\u0026nbsp;\u003C\/p\u003E\u003Cp\u003EIn partial fulfillment of the requirements for the degree of\u003C\/p\u003E\u003Cp\u003E\u0026nbsp;\u003C\/p\u003E\u003Cp\u003EDoctor of Philosophy in Biology\u003C\/p\u003E\u003Cp\u003Ein the\u003C\/p\u003E\u003Cp\u003ESchool of Biological Sciences\u003C\/p\u003E\u003Cp\u003E\u0026nbsp;\u003C\/p\u003E\u003Cp\u003E\u003Cstrong\u003ELing Zhu\u003C\/strong\u003E\u003C\/p\u003E\u003Cp\u003E\u0026nbsp;\u003C\/p\u003E\u003Cp\u003EWill defend her dissertation\u003C\/p\u003E\u003Cp\u003E\u0026nbsp;\u003Cstrong\u003EMolecular signatures of histone hyperacetylation in embryonic stem cells\u003C\/strong\u003E\u0026nbsp;\u003C\/p\u003E\u003Cp\u003E\u0026nbsp;\u003C\/p\u003E\u003Cp\u003ETime and Date: 1:30PM (Eastern Time), February 25th, 2025\u003Cbr\u003ELocation: IBB 2316\u003C\/p\u003E\u003Cp\u003E\u0026nbsp;\u003C\/p\u003E\u003Cp\u003EZoom Link:\u0026nbsp;\u003Ca href=\u0022https:\/\/gatech.zoom.us\/j\/95234911048?pwd=vwOecaXtpgRJU7vaY3j9crV7ahL089.1\u0022\u003EJoin Zoom Meeting\u003C\/a\u003E\u003C\/p\u003E\u003Cp\u003EMeeting ID: 952 3491 1048\u003C\/p\u003E\u003Cp\u003EPasscode: 743346\u003C\/p\u003E\u003Cp\u003E\u003Cstrong\u003E\u0026nbsp;\u003C\/strong\u003E\u003C\/p\u003E\u003Cp\u003E\u0026nbsp;\u003C\/p\u003E\u003Cp\u003E\u0026nbsp;\u003C\/p\u003E\u003Cp\u003E\u003Cstrong\u003E\u0026nbsp;Thesis Advisor:\u003C\/strong\u003E\u003C\/p\u003E\u003Cp\u003EYuhong Fan, Ph.D.\u003C\/p\u003E\u003Cp\u003ESchool of Biological Sciences\u003C\/p\u003E\u003Cp\u003EGeorgia Institute of Technology\u003C\/p\u003E\u003Cp\u003E\u0026nbsp;\u003C\/p\u003E\u003Cp\u003E\u003Cstrong\u003ECommittee Members:\u003C\/strong\u003E\u003C\/p\u003E\u003Cp\u003EShuyi Nie, Ph.D.\u003C\/p\u003E\u003Cp\u003ESchool of Biological Sciences\u003C\/p\u003E\u003Cp\u003EGeorgia Institute of Technology\u003C\/p\u003E\u003Cp\u003E\u0026nbsp;\u003C\/p\u003E\u003Cp\u003EKaixiang Cao, Ph.D.\u003C\/p\u003E\u003Cp\u003ESchool of Medicine\u003C\/p\u003E\u003Cp\u003ECase Western Reserve University\u003C\/p\u003E\u003Cp\u003E\u0026nbsp;\u003C\/p\u003E\u003Cp\u003EHengbin Wang, Ph.D.\u003C\/p\u003E\u003Cp\u003ESchool of Medicine Internal Medicine\u003Cbr\u003EVirginia Commonwealth University\u003C\/p\u003E\u003Cp\u003E\u0026nbsp;\u003C\/p\u003E\u003Cp\u003EJiyue Zhu, Ph.D.\u003C\/p\u003E\u003Cp\u003ESchool of Pharmacy and Pharmaceutical Sciences\u003C\/p\u003E\u003Cp\u003EWashington State University\u003C\/p\u003E\u003Cp\u003E\u0026nbsp;\u003C\/p\u003E\u003Cp\u003E\u003Cstrong\u003EABSTRACT:\u003C\/strong\u003E\u003C\/p\u003E\u003Cp\u003EHistone acetylation is a key epigenetic modification that plays a pivotal role in gene expression and stem cell differentiation. This dissertation investigates its regulatory mechanisms by leveraging a genetically encoded histone acetylation biosensor combined with single-cell RNA sequencing of embryonic stem cells (ESCs) and bioinformatics analysis to decipher molecular signatures of histone hyperacetylation in ESCs. Our findings reveal distinct gene expression patterns associated with specific hyperacetylated histones, particularly in genes controlling pluripotency, germ layer formation, development, differentiation, and aging, suggesting histone acetylation plays a crucial role in controlling these processes. Additionally, we examined molecular signatures and the dynamic expression of H1 linker histones in ESCs undergoing histone hyperacetylation induced by acute treatment with the HDAC inhibitor Valproic Acid (VPA). VPA treatment causes histone hyperacetylation and triggers transcriptional signatures indicative of early differentiation and lineage commitment. Quantitative analysis using reverse-phase high performance liquid chromatography and mass spectrometry showed that histone hyperacetylation in ESCs increases total H1 levels and alters H1 variant expression. Further, VPA-induced histone hyperacetylation links to characteristic changes of H1 variants in chromatin, differing from those in embryoid body differentiation, suggesting distinct chromatin remodeling pathways underlying these ESC differentiation processes.\u003C\/p\u003E\u003Cp\u003EOverall, our results demonstrate that histone hyperacetylation facilitates chromatin remodeling and coordinates with H1 linker histones to regulate transcriptional programs governing stem cell differentiation and fate determination. These molecular signatures highlight its role in chromatin reorganization and gene regulation.\u003C\/p\u003E\u003Cp\u003E\u0026nbsp;\u003C\/p\u003E","summary":"","format":"limited_html"}],"field_subtitle":"","field_summary":[{"value":"\u003Cp\u003E\u003Cstrong\u003EMolecular signatures of histone hyperacetylation in embryonic stem cells\u003C\/strong\u003E\u0026nbsp;\u003C\/p\u003E","format":"limited_html"}],"field_summary_sentence":[{"value":"Molecular signatures of histone hyperacetylation in embryonic stem cells "}],"uid":"27707","created_gmt":"2025-02-19 15:35:38","changed_gmt":"2025-02-19 15:36:19","author":"Tatianna Richardson","boilerplate_text":"","field_publication":"","field_article_url":"","field_event_time":{"event_time_start":"2025-02-25T13:30:00-05:00","event_time_end":"2025-02-25T15:00:00-05:00","event_time_end_last":"2025-02-25T15:00:00-05:00","gmt_time_start":"2025-02-25 18:30:00","gmt_time_end":"2025-02-25 20:00:00","gmt_time_end_last":"2025-02-25 20:00:00","rrule":null,"timezone":"America\/New_York"},"location":"IBB 2316","extras":[],"groups":[{"id":"221981","name":"Graduate Studies"}],"categories":[],"keywords":[{"id":"100811","name":"Phd Defense"}],"core_research_areas":[],"news_room_topics":[],"event_categories":[{"id":"1788","name":"Other\/Miscellaneous"}],"invited_audience":[{"id":"78771","name":"Public"}],"affiliations":[],"classification":[],"areas_of_expertise":[],"news_and_recent_appearances":[],"phone":[],"contact":[],"email":[],"slides":[],"orientation":[],"userdata":""}}}