{"680555":{"#nid":"680555","#data":{"type":"event","title":"BioE PhD Proposal Presentation- Jiho Seok","body":[{"value":"\u003Cp\u003E\u003Cstrong\u003EAdvisor:\u0026nbsp;\u003C\/strong\u003EMark Styczynski, Ph.D. (School of Chemical and Biomolecular Engineering, Georgia Tech)\u003C\/p\u003E\u003Cp\u003E\u003Cstrong\u003ECommittee Members:\u003C\/strong\u003E\u003C\/p\u003E\u003Cp\u003EJohn Blazeck, Ph.D. (School of Chemical and Biomolecular Engineering, Georgia Tech)\u003C\/p\u003E\u003Cp\u003ELily Cheung, Ph.D. (School of Chemical and Biomolecular Engineering, Georgia Tech)\u003C\/p\u003E\u003Cp\u003EGabriel Kwong, Ph.D. (Wallace H. Coulter Department of Biomedical Engineering, Georgia Tech )\u003C\/p\u003E\u003Cp\u003EBrian Hammer, Ph.D. (School of Biological Sciences, Georgia Tech)\u0026nbsp;\u003C\/p\u003E\u003Cp\u003E\u003Cstrong\u003EMultifaceted Rolling Circle Amplification Strategies to Reduce False Positives in Point-of-Care Biosensors\u003C\/strong\u003E\u003C\/p\u003E\u003Cp\u003EIn modern warfare, developing sensors that can be used in the field to address the risks associated with biological weapons and infectious diseases is essential. Densely populated military bases enable the rapid spread of infectious diseases, requiring rapid and accurate detection despite limited resources. Particularly, false positives in a sensor result in a significant waste of workforce, time, and resources. Therefore, developing sensors with minimal false positives and ease of use is essential. Rolling Circle Amplification (RCA) is an isothermal DNA amplification technology making it ideal for point-of-care (POC) biosensors. However, RCA performed in a minimal-equipment POC setting operates at a temperature low enough to increase the rate of false positives, presenting an inherent limitation to its utility at the POC. Therefore, I propose a multifaceted approach to reduce false positives in RCA through the multiplexed detection of various targets while maintaining suitability for field deployment. First, I propose a strategy to enhance input selectivity to detect target pathogens accurately without false positives. I propose a novel approach using an AND gate to detect multiple genes simultaneously. Next, I will improve selectivity for a single gene by combining a gap-filling technique with mismatch endonucleases to require precise sequence complementarity for amplification. Then, I will reduce false positives by diversifying output signals for multiple targets within the same pathogen by combining Rolling Circle Transcription (RCT) with RNA fluorescent aptamers. For all of these approaches, I will aim to achieve field deployability by integrating the multiple reaction steps of RCA into one step and demonstrating functionality after lyophilization. Once successfully completed, the work proposed here will enable the development of biosensors that minimize false positives, are user-friendly, and present practical sensor technology applicable in the field.\u003C\/p\u003E","summary":"","format":"limited_html"}],"field_subtitle":"","field_summary":[{"value":"\u003Cp\u003EBioE PhD Proposal Presentation- \u0022Multifaceted Rolling Circle Amplification Strategies to Reduce False Positives in Point-of-Care Biosensors\u0022 - Jiho Seok\u003C\/p\u003E","format":"limited_html"}],"field_summary_sentence":[{"value":"\u0022Multifaceted Rolling Circle Amplification Strategies to Reduce False Positives in Point-of-Care Biosensors\u0022"}],"uid":"27917","created_gmt":"2025-02-18 14:37:18","changed_gmt":"2025-02-18 14:38:24","author":"Laura Paige","boilerplate_text":"","field_publication":"","field_article_url":"","field_event_time":{"event_time_start":"2025-03-06T15:00:00-05:00","event_time_end":"2025-03-06T17:00:00-05:00","event_time_end_last":"2025-03-06T17:00:00-05:00","gmt_time_start":"2025-03-06 20:00:00","gmt_time_end":"2025-03-06 22:00:00","gmt_time_end_last":"2025-03-06 22:00:00","rrule":null,"timezone":"America\/New_York"},"location":"4029 EBB","extras":[],"groups":[{"id":"65448","name":"Bioengineering Graduate Program"}],"categories":[],"keywords":[{"id":"172056","name":"go-BioE"}],"core_research_areas":[],"news_room_topics":[],"event_categories":[],"invited_audience":[],"affiliations":[],"classification":[],"areas_of_expertise":[],"news_and_recent_appearances":[],"phone":[],"contact":[],"email":[],"slides":[],"orientation":[],"userdata":""}}}