{"679616":{"#nid":"679616","#data":{"type":"event","title":"The School of Biological Sciences Spring 2025 Seminar Series presents Dr. Min Kyung Shinn","body":[{"value":"\u003Cdiv\u003E\u003Cdiv\u003E\u003Cdiv\u003E\u003Cdiv\u003E\u003Cdiv\u003E\u003Cstrong\u003EAbstract\u003C\/strong\u003E: Nuclear speckles organize into 20-50 irregularly shaped structures on the sub-micron-scale and are enriched in serine\/arginine-rich splicing factors (SRSFs) involved in mRNA splicing. Speckle-associated RNA binding proteins (RBPs) have multi-domain architectures comprising one or two folded RNA recognition motifs and intrinsically disordered regions. The molecular architectures of SRSFs map onto di- or tetra-block copolymers, driving microphase separation above a threshold concentration. Accordingly, we observe the formation of size-limited, ordered assemblies that result from a balance of attractive and repulsive interactions among the SRSF domains. Structural investigations using freeze-fracture deep-etch electron microscopy show assemblies of 30-40 nm in diameter with distinct morphologies. We also observe that microphases can associate to form sub-micron scale clusters by short-range attractive and long-range repulsive interactions that are disrupted by the presence of a co-ion. We also find that the speckle-enriched long non-coding RNA MALAT1 and the C-terminal domain of the RNA polymerase II promote microphase separation of SRSF1 by lowering the threshold concentration. This suggests a model for a molecular mechanism of interplay among the essential components involved in RNA processing.\u003C\/div\u003E\u003C\/div\u003E\u003C\/div\u003E\u003C\/div\u003E\u003C\/div\u003E\u003Cdiv\u003E\u003Cdiv\u003E\u003Cdiv\u003E\u0026nbsp;\u003C\/div\u003E\u003C\/div\u003E\u003C\/div\u003E","summary":"","format":"limited_html"}],"field_subtitle":"","field_summary":[{"value":"\u003Cp\u003ENuclear speckle proteins undergo intrinsic and RNA-dependent microphase separation\u003C\/p\u003E","format":"limited_html"}],"field_summary_sentence":[{"value":"Nuclear speckle proteins undergo intrinsic and RNA-dependent microphase separation"}],"uid":"36696","created_gmt":"2025-01-15 15:38:15","changed_gmt":"2025-02-21 19:30:27","author":"ebossard3","boilerplate_text":"","field_publication":"","field_article_url":"","field_event_time":{"event_time_start":"2025-01-23T11:00:00-05:00","event_time_end":"2025-01-23T12:00:00-05:00","event_time_end_last":"2025-01-23T12:00:00-05:00","gmt_time_start":"2025-01-23 16:00:00","gmt_time_end":"2025-01-23 17:00:00","gmt_time_end_last":"2025-01-23 17:00:00","rrule":null,"timezone":"America\/New_York"},"location":"EBB 1005","extras":["free_food"],"hg_media":{"676057":{"id":"676057","type":"image","title":"Min-Kyung-Shinn.png","body":null,"created":"1737137178","gmt_created":"2025-01-17 18:06:18","changed":"1737137178","gmt_changed":"2025-01-17 18:06:18","alt":"Min-Kyung-Shinn.png","file":{"fid":"259764","name":"Min-Kyung-Shinn.png","image_path":"\/sites\/default\/files\/2025\/01\/17\/Min-Kyung-Shinn.png","image_full_path":"http:\/\/hg.gatech.edu\/\/sites\/default\/files\/2025\/01\/17\/Min-Kyung-Shinn.png","mime":"image\/png","size":83227,"path_740":"http:\/\/hg.gatech.edu\/sites\/default\/files\/styles\/740xx_scale\/public\/2025\/01\/17\/Min-Kyung-Shinn.png?itok=b1DSH7Ax"}}},"media_ids":["676057"],"groups":[{"id":"1275","name":"School of Biological Sciences"}],"categories":[],"keywords":[{"id":"172458","name":"biological sciences"}],"core_research_areas":[],"news_room_topics":[],"event_categories":[{"id":"1795","name":"Seminar\/Lecture\/Colloquium"}],"invited_audience":[{"id":"78761","name":"Faculty\/Staff"},{"id":"177814","name":"Postdoc"},{"id":"78771","name":"Public"},{"id":"174045","name":"Graduate students"},{"id":"78751","name":"Undergraduate students"}],"affiliations":[],"classification":[],"areas_of_expertise":[],"news_and_recent_appearances":[],"phone":[],"contact":[{"value":"\u003Cp\u003Erbailey74@gatech.edu\u003C\/p\u003E","format":"limited_html"}],"email":[],"slides":[],"orientation":[],"userdata":""}}}