{"676856":{"#nid":"676856","#data":{"type":"event","title":"Divisional Seminar - Dr. Leah Bushin (UC San Diego)","body":[{"value":"\u003Cdiv\u003E\u003Cstrong\u003EGrowth-coupled Microbial Biosynthesis of the Animal pPgment Xanthommatin\u003C\/strong\u003E\u003C\/div\u003E\u003Cdiv\u003E\u0026nbsp;\u003C\/div\u003E\u003Cdiv\u003E\u003Cstrong\u003EAbstract:\u003C\/strong\u003E\u003C\/div\u003E\u003Cdiv\u003EThe mining of genomes across life has unearthed a bounty of biosynthetic potential to diverse molecules key to a biobased future. While the heterologous expression of metabolic pathways has achieved broad success, most approaches suffer a similar fate in low initial production levels that require extensive, resource-heavy iterative strain engineering refinement. Herein we introduce a growth-coupled biosynthetic (GrowBio) strategy that irrevocably connects microbial growth with specialized compound production. We demonstrate the plug-and-play versatility of GrowBio in the production of the structurally complex animal biopigment xanthommatin, a color-changing ommochrome with material and cosmetic potential. Xanthommatin biosynthesis directly fuels growth of a newly designed Pseudomonas putida 5,10-methylenetetrahydrofolate auxotroph (PUMA). Aided by genome-scale metabolic modeling, PUMA was designed and built to be controlled by endogenous formate co-produced as a coupled biosynthetic byproduct in the multistep conversion of tryptophan to xanthommatin. Adaptive laboratory evolution was utilized to streamline xanthommatin\u0027s gram-scale bioproduction via growth rate selection, establishing GrowBio as a promising biotechnological approach for establishing and optimizing the microbial production of value-added molecules.\u003C\/div\u003E\u003Cdiv\u003E\u003Cbr\u003E\u003Cbr\u003E\u0026nbsp;\u003C\/div\u003E","summary":"","format":"limited_html"}],"field_subtitle":"","field_summary":[{"value":"\u003Cp\u003EOne in the series of the SoCB seminars.\u003C\/p\u003E","format":"limited_html"}],"field_summary_sentence":[{"value":"School of Chemistry \u0026 Biochemistry -Seminar Series"}],"uid":"36441","created_gmt":"2024-09-17 13:17:42","changed_gmt":"2024-10-07 19:05:21","author":"slawrence67","boilerplate_text":"","field_publication":"","field_article_url":"","field_event_time":{"event_time_start":"2024-10-15T11:00:00-04:00","event_time_end":"2024-10-15T12:00:00-04:00","event_time_end_last":"2024-10-15T12:00:00-04:00","gmt_time_start":"2024-10-15 15:00:00","gmt_time_end":"2024-10-15 16:00:00","gmt_time_end_last":"2024-10-15 16:00:00","rrule":null,"timezone":"America\/New_York"},"location":"MoSE 3201A","extras":[],"groups":[{"id":"85951","name":"School of Chemistry and Biochemistry"}],"categories":[],"keywords":[],"core_research_areas":[],"news_room_topics":[],"event_categories":[{"id":"1795","name":"Seminar\/Lecture\/Colloquium"}],"invited_audience":[{"id":"78761","name":"Faculty\/Staff"},{"id":"177814","name":"Postdoc"},{"id":"174045","name":"Graduate students"},{"id":"78751","name":"Undergraduate students"}],"affiliations":[],"classification":[],"areas_of_expertise":[],"news_and_recent_appearances":[],"phone":[],"contact":[{"value":"\u003Cp\u003E\u003Cstrong\u003EHost\u003C\/strong\u003E: Prof. Vinayak Agarwal\u003C\/p\u003E","format":"limited_html"}],"email":[],"slides":[],"orientation":[],"userdata":""}}}