{"675267":{"#nid":"675267","#data":{"type":"event","title":"MS Presentation by Jacob Hewes","body":[{"value":"\u003Cp\u003E\u003Cstrong\u003EJacob Hewes\u003C\/strong\u003E\u003C\/p\u003E\u003Cp\u003E\u003Cstrong\u003EBioE MS Thesis Presentation\u003C\/strong\u003E\u003C\/p\u003E\u003Cp\u003EThursday, July 18th, 2024, 1:30 PM\u003C\/p\u003E\u003Cp\u003EIBB 3316\u003C\/p\u003E\u003Cp\u003E\u003Ca href=\u0022https:\/\/gatech.zoom.us\/j\/96045411587?pwd=hZJ6E6XuOEtCbbwiNNXlmgDUBwSDPa.1\u0022\u003Ehttps:\/\/gatech.zoom.us\/j\/96045411587?pwd=hZJ6E6XuOEtCbbwiNNXlmgDUBwSDPa.1\u003C\/a\u003E\u003C\/p\u003E\u003Cp\u003EMeeting ID: 960 4541 1587\u003C\/p\u003E\u003Cp\u003EPasscode: 109969\u003C\/p\u003E\u003Cp\u003E\u003Cstrong\u003EThesis Advisor:\u003C\/strong\u003E\u003C\/p\u003E\u003Cp\u003EAndr\u00e9s J. Garc\u00eda, Ph.D., George W. Woodruff School of Mechanical Engineering, Georgia\u003C\/p\u003E\u003Cp\u003EInstitute of Technology\u003C\/p\u003E\u003Cp\u003E\u0026nbsp;\u003C\/p\u003E\u003Cp\u003E\u003Cstrong\u003EThesis Committee Members:\u003C\/strong\u003E\u003C\/p\u003E\u003Cp\u003EHang Lu, Ph.D., School of Chemical and Biomolecular Engineering, Georgia Institute of\u003C\/p\u003E\u003Cp\u003ETechnology\u003C\/p\u003E\u003Cp\u003EAnkur Singh, Ph.D., George W. Woodruff School of Mechanical Engineering, Georgia Institute\u003C\/p\u003E\u003Cp\u003Eof Technology\u003C\/p\u003E\u003Cp\u003E\u0026nbsp;\u003C\/p\u003E\u003Cp\u003E\u003Cstrong\u003ESynthetic PEG-Maleimide Hydrogel For \u003C\/strong\u003E\u003Cem\u003E\u003Cstrong\u003EIn vitro\u0026nbsp;\u003C\/strong\u003E\u003C\/em\u003E\u003Cstrong\u003ECulture of Primary Human\u003C\/strong\u003E\u003C\/p\u003E\u003Cp\u003E\u003Cstrong\u003EIntestinal Enteroids\u003C\/strong\u003E\u003C\/p\u003E\u003Cp\u003EGastrointestinal diseases are becoming increasingly prevalent in developed countries,\u003C\/p\u003E\u003Cp\u003Estimulating the need for human-specific models of intestinal development and disease that can\u003C\/p\u003E\u003Cp\u003Erecapitulate the structure and function of the gut in vitro. In the past decade, intestinal organoid\u003C\/p\u003E\u003Cp\u003Etechnology has advanced in vitro reproduction of intestinal organoids. Enteroids, epithelial\u003C\/p\u003E\u003Cp\u003Eorganoids derived from human intestinal tissue, are three-dimensional (3D) structures that can\u003C\/p\u003E\u003Cp\u003Emodel the identity, cell heterogeneity, and cell behaviors of the original tissue in vitro. This\u003C\/p\u003E\u003Cp\u003Emakes them a powerful tool for drug screening, disease modeling, and reconstructing damaged\u003C\/p\u003E\u003Cp\u003Eepithelium in conditions like ulcerative colitis.\u003C\/p\u003E\u003Cp\u003ECurrent protocols for organoid culture require expansion of intestinal stem cells within\u003C\/p\u003E\u003Cp\u003EMatrigel, a tumor-derived extracellular matrix (ECM) that exhibits considerable lot-to-lot\u003C\/p\u003E\u003Cp\u003Evariability, poor experimental control, and inability to decouple matrix physical and biochemical\u003C\/p\u003E\u003Cp\u003Eproperties due to its ill-defined composition. The reliance on Matrigel for intestinal organoid\u003C\/p\u003E\u003Cp\u003Eculture severely limits their translational potential. This thesis project aims to reduce the\u003C\/p\u003E\u003Cp\u003Erequirement for biologically-derived ECMs to support intestinal organoid culture. To accomplish\u003C\/p\u003E\u003Cp\u003Ethis aim, we developed completely synthetic hydrogels presenting ECM-derived adhesive\u003C\/p\u003E\u003Cp\u003Eligands crosslinked with peptides susceptible to matrix metalloprotease (MMP) degradation to\u003C\/p\u003E\u003Cp\u003Eidentify gel compositions supporting the culture of enteroids starting from human tissue-derived\u003C\/p\u003E\u003Cp\u003Eprogenitor epithelial cells.\u003C\/p\u003E\u003Cp\u003EThe synthetic hydrogel platforms designed were based on a four-arm poly(ethylene\u003C\/p\u003E\u003Cp\u003Eglycol) (PEG) macromer with maleimide groups at each terminus (PEG-4MAL) and the RGD\u003C\/p\u003E\u003Cp\u003Eintegrin-binding peptide. Hydrogel biophysical properties and crosslinker type were key\u003C\/p\u003E\u003Cp\u003Eparameters in engineering a synthetic ECM mimic that supported human ileum enteroids. A\u003C\/p\u003E\u003Cp\u003EPEG-4MAL hydrogel platform with the protease-degradable crosslinker IPES promoted the best\u003C\/p\u003E\u003Cp\u003Eenteroid emergence and growth compared to Matrigel. In this synthetic matrix, human intestinal\u003C\/p\u003E\u003Cp\u003Eenteroids emerge from single cells and express markers of intestinal stem cells. The modular\u003C\/p\u003E\u003Cp\u003Edesign of this synthetic matrix and its ability to support the in vitro culture of enteroids\u003C\/p\u003E\u003Cp\u003Estrengthens the translational potential of this platform for regenerative medicine, disease\u003C\/p\u003E\u003Cp\u003Emodeling, and other applications while reducing the dependency on Matrigel.\u003C\/p\u003E","summary":"","format":"limited_html"}],"field_subtitle":"","field_summary":[{"value":"\u003Cp\u003ESee Below\u003C\/p\u003E","format":"limited_html"}],"field_summary_sentence":[{"value":" Synthetic PEG-Maleimide Hydrogel For In vitro Culture of Primary Human Intestinal Enteroids"}],"uid":"27707","created_gmt":"2024-06-28 14:35:47","changed_gmt":"2024-06-28 14:36:15","author":"Tatianna Richardson","boilerplate_text":"","field_publication":"","field_article_url":"","field_event_time":{"event_time_start":"2024-07-18T13:30:00-04:00","event_time_end":"2024-07-18T15:30:00-04:00","event_time_end_last":"2024-07-18T15:30:00-04:00","gmt_time_start":"2024-07-18 17:30:00","gmt_time_end":"2024-07-18 19:30:00","gmt_time_end_last":"2024-07-18 19:30:00","rrule":null,"timezone":"America\/New_York"},"location":"IBB 3316","extras":[],"groups":[{"id":"221981","name":"Graduate Studies"}],"categories":[],"keywords":[{"id":"111531","name":"ms defense"}],"core_research_areas":[],"news_room_topics":[],"event_categories":[{"id":"1788","name":"Other\/Miscellaneous"}],"invited_audience":[{"id":"78771","name":"Public"}],"affiliations":[],"classification":[],"areas_of_expertise":[],"news_and_recent_appearances":[],"phone":[],"contact":[],"email":[],"slides":[],"orientation":[],"userdata":""}}}