{"673663":{"#nid":"673663","#data":{"type":"event","title":"Colloquium Seminar - Prof. Lu Wei (Caltech)","body":[{"value":"\u003Cp\u003E\u003Cstrong\u003E\u003Cspan\u003E\u003Cspan\u003E\u003Cspan\u003EFunctional Bond-Selective Microscopy for Subcellular Biology: from quantitative imaging and sensing to single-molecule analysis\u003C\/span\u003E\u003C\/span\u003E\u003C\/span\u003E\u003C\/strong\u003E\u003C\/p\u003E\r\n\r\n\u003Cp\u003E\u003Cstrong\u003E\u003Cspan\u003E\u003Cspan\u003E\u003Cspan\u003EAbstract:\u003C\/span\u003E\u003C\/span\u003E\u003C\/span\u003E\u003C\/strong\u003E\u003C\/p\u003E\r\n\r\n\u003Cp\u003E\u003Cspan\u003E\u003Cspan\u003E\u003Cspan\u003EAdvances in optical spectroscopy and microscopy have revolutionized our understanding in live\u003C\/span\u003E\u003C\/span\u003E\u003C\/span\u003E \u003Cspan\u003E\u003Cspan\u003E\u003Cspan\u003Ebiological functions at the sub-cellular levels. In this seminar, I will present our recent efforts in developing and applying next-generation bond-selective spectro-microscopy for retrieving functional chemical information in live cells and neurons. I will first discuss the coupling of the stimulated Raman scattering (SRS) imaging, a nonlinear Raman imaging modality, with newly developed biorthogonal chemical strategies for \u003C\/span\u003E\u003C\/span\u003E\u003C\/span\u003E\u003Cspan\u003E\u003Cspan\u003E\u003Cspan\u003Equantitative subcellular analysis of protein aggregates\u003C\/span\u003E\u003C\/span\u003E\u003C\/span\u003E \u003Cspan\u003E\u003Cspan\u003E\u003Cspan\u003Ein neurodegenerative diseases and for intracellular local environmental sensing through alkyne-\u003Cspan\u003E hydrogen\u2013deuterium exchange\u003C\/span\u003E (Alkyne-HDX). I will then present a new mid-infrared near-infrared double-resonance imaging technique, BonFIRE, for bond-selective fluorescence imaging with single-molecule sensitivity. This microscopy platform will allow new capabilities for wide-field super-multiplex imaging and vibrational life-time imaging for sensing heterogeneous cellular interactions and environment.\u0026nbsp; \u003C\/span\u003E\u003C\/span\u003E\u003C\/span\u003E\u003C\/p\u003E\r\n","summary":"","format":"limited_html"}],"field_subtitle":"","field_summary":[{"value":"\u003Cp\u003EOne in the series of the SoCB seminars.\u003C\/p\u003E\r\n","format":"limited_html"}],"field_summary_sentence":[{"value":"School of Chemistry \u0026 Biochemistry -Seminar Series"}],"uid":"36441","created_gmt":"2024-03-21 16:09:15","changed_gmt":"2024-03-21 16:09:15","author":"slawrence67","boilerplate_text":"","field_publication":"","field_article_url":"","field_event_time":{"event_time_start":"2024-04-11T15:30:00-04:00","event_time_end":"2024-04-11T16:30:00-04:00","event_time_end_last":"2024-04-11T16:30:00-04:00","gmt_time_start":"2024-04-11 19:30:00","gmt_time_end":"2024-04-11 20:30:00","gmt_time_end_last":"2024-04-11 20:30:00","rrule":null,"timezone":"America\/New_York"},"location":"MoSE - G011","extras":[],"groups":[{"id":"85951","name":"School of Chemistry and Biochemistry"}],"categories":[],"keywords":[],"core_research_areas":[],"news_room_topics":[],"event_categories":[{"id":"1795","name":"Seminar\/Lecture\/Colloquium"}],"invited_audience":[{"id":"78761","name":"Faculty\/Staff"},{"id":"174045","name":"Graduate students"},{"id":"177814","name":"Postdoc"},{"id":"78751","name":"Undergraduate students"}],"affiliations":[],"classification":[],"areas_of_expertise":[],"news_and_recent_appearances":[],"phone":[],"contact":[{"value":"\u003Cp\u003EProf. Marcus Ciccerone\u003C\/p\u003E\r\n","format":"limited_html"}],"email":[],"slides":[],"orientation":[],"userdata":""}}}