{"672841":{"#nid":"672841","#data":{"type":"event","title":"Special Seminar - Dr. Vicki Wysocki (Ohio State University) - Chair Candidate","body":[{"value":"\u003Cdiv\u003E\u0026nbsp;\u003C\/div\u003E\r\n\r\n\u003Cp\u003E\u003Cstrong\u003E\u003Cspan\u003E\u003Cem\u003E\u003Cspan\u003E\u003Cspan\u003E\u003Cspan\u003E\u003Cspan\u003ENative Mass Spectrometry: a Structural Biology Tool\u003C\/span\u003E\u003C\/span\u003E\u003C\/span\u003E\u003C\/span\u003E\u003C\/em\u003E\u003C\/span\u003E\u003C\/strong\u003E\u003C\/p\u003E\r\n\r\n\u003Cp\u003E\u003Cstrong\u003E\u003Cspan\u003E\u003Cspan\u003E\u003Cspan\u003E\u003Cspan\u003E\u003Cspan\u003EAbstract:\u003C\/span\u003E\u003C\/span\u003E\u003C\/span\u003E\u003C\/span\u003E\u003C\/span\u003E\u003C\/strong\u003E\u003C\/p\u003E\r\n\r\n\u003Cdiv\u003E\u0026nbsp;\u003C\/div\u003E\r\n\r\n\u003Cp\u003E\u003Cspan\u003E\u003Cspan\u003E\u003Cspan\u003E\u003Cspan\u003E\u003Cspan\u003E\u003Cspan\u003ECharacterization of the overall topology and inter-subunit contacts of protein complexes, and their assembly\/ disassembly and unfolding pathways, is critical because protein complexes regulate key biological processes, including processes important in understanding and controlling disease. Tools to address structural biology problems continue to improve.\u0026nbsp; Native mass spectrometry (nMS) and associated technologies such as ion mobility and variable temperature electrospray ionization are becoming increasingly important components of the structural biology toolbox.\u0026nbsp; When the native mass spectrometry approach is used early or mid-course in a structural characterization project, it can provides answers quickly\u0026nbsp; for small amounts of sample, even when heterogeneity is present. Integration of sample preparation\/purification with effective dissociation methods (e.g., surface-induced dissociation, SID), ion mobility, and computational approaches provide an MS workflow that is enabling in biochemical, synthetic biology, and systems biology approaches. Native MS can determine whether a complex of interest exists in a single or multiple oligomeric states, and surface induced dissociation can provide characterization of topology\/inter-subunit connectivity and other structural features.\u0026nbsp; Examples will illustrate the coupling of SID to electron capture charge reduction and charge detection mass spectrometry for the characterization of protein and nucleoprotein complexes, including glycoproteins and adeno-associated virus capsids.\u003Cbr \/\u003E\r\n\u003Cbr \/\u003E\r\nReferences\u003Cbr \/\u003E\r\n[1] Snyder, Harvey, Wysocki, V. H., Chemical Reviews, 2022, 122, 8, 7442\u20137487.\u003Cbr \/\u003E\r\n[2] Karch, Snyder, Harvey, Wysocki Annu. Rev. Biophys. 2022. 51, 157\u201379.\u003C\/span\u003E\u003C\/span\u003E\u003C\/span\u003E\u003C\/span\u003E\u003C\/span\u003E\u003C\/span\u003E\u003C\/p\u003E\r\n","summary":"","format":"limited_html"}],"field_subtitle":"","field_summary":[{"value":"\u003Cp\u003ESoCB search for school Chair.\u003C\/p\u003E\r\n","format":"limited_html"}],"field_summary_sentence":[{"value":"College of Sciences - Special Seminar"}],"uid":"36441","created_gmt":"2024-02-08 21:02:32","changed_gmt":"2024-02-08 21:04:28","author":"slawrence67","boilerplate_text":"","field_publication":"","field_article_url":"","field_event_time":{"event_time_start":"2024-02-14T15:30:00-05:00","event_time_end":"2024-02-14T16:30:00-05:00","event_time_end_last":"2024-02-14T16:30:00-05:00","gmt_time_start":"2024-02-14 20:30:00","gmt_time_end":"2024-02-14 21:30:00","gmt_time_end_last":"2024-02-14 21:30:00","rrule":null,"timezone":"America\/New_York"},"location":"EBB, Room #1005","extras":[],"groups":[{"id":"85951","name":"School of Chemistry and Biochemistry"}],"categories":[],"keywords":[],"core_research_areas":[],"news_room_topics":[],"event_categories":[{"id":"1795","name":"Seminar\/Lecture\/Colloquium"}],"invited_audience":[{"id":"78761","name":"Faculty\/Staff"},{"id":"177814","name":"Postdoc"},{"id":"78771","name":"Public"},{"id":"174045","name":"Graduate students"},{"id":"78751","name":"Undergraduate students"}],"affiliations":[],"classification":[],"areas_of_expertise":[],"news_and_recent_appearances":[],"phone":[],"contact":[],"email":[],"slides":[],"orientation":[],"userdata":""}}}