{"671958":{"#nid":"671958","#data":{"type":"event","title":"Special Seminar - Professor Rivka Isaacson ( King\u0027s College, London)","body":[{"value":"\u003Cp\u003E\u003Cspan\u003E\u003Cspan\u003E\u003Cspan\u003E\u003Cstrong\u003EMolecular Machinery for Proteostasis within the Crowded Cell\u003C\/strong\u003E\u003C\/span\u003E\u003C\/span\u003E\u003C\/span\u003E\u003C\/p\u003E\r\n\r\n\u003Cp\u003E\u003Cspan\u003E\u003Cspan\u003E\u003Cspan\u003E\u003Cstrong\u003EAbstract:\u003C\/strong\u003E\u003C\/span\u003E\u003C\/span\u003E\u003C\/span\u003E\u003C\/p\u003E\r\n\r\n\u003Cp\u003E\u003Cspan\u003E\u003Cspan\u003E\u003Cspan\u003EThe crowded cell interior relies on many quality control mechanisms to ensure the correct protein machinery is present in the right places at the right times. I will present two projects currently underway in our lab that unite within this theme. We use a range of biophysics techniques, including NMR, X-ray crystallography, cryo-electron microscopy, SAXS, EPR, native mass-spectrometry, and more, to study structure, function and interactions of proteins involved in these processes.\u003C\/span\u003E\u003C\/span\u003E\u003C\/span\u003E\u003C\/p\u003E\r\n\r\n\u003Cp\u003E\u003Cspan\u003E\u003Cspan\u003E\u003Cspan\u003E\u003Cem\u003EMetabolic Shutdown in Sporulation\u003C\/em\u003E\u003C\/span\u003E\u003C\/span\u003E\u003C\/span\u003E\u003C\/p\u003E\r\n\r\n\u003Cp\u003E\u003Cspan\u003E\u003Cspan\u003E\u003Cspan\u003EWhen certain bacteria find themselves in unfavourable growth conditions such as nutrient deprivation, they make a carefully choreographed lifestyle switch to a hardy dormant form called a spore which will survive harsh conditions and revive when the time is ripe. One aspect of sporulation is metabolic shutdown of the developing spore and I will present some work we have been doing to understand this process in \u003Cem\u003EBacillus subtilis\u003C\/em\u003E.\u003C\/span\u003E\u003C\/span\u003E\u003C\/span\u003E\u003C\/p\u003E\r\n\r\n\u003Cp\u003E\u003Cspan\u003E\u003Cspan\u003E\u003Cspan\u003E\u003Cem\u003ETriage of Mislocalised Proteins\u003C\/em\u003E\u003C\/span\u003E\u003C\/span\u003E\u003C\/span\u003E\u003C\/p\u003E\r\n\r\n\u003Cp\u003E\u003Cspan\u003E\u003Cspan\u003E\u003Cspan\u003EIn mammalian cells, a co-chaperone called SGTA (small, glutamine-rich, tetratricopeptide repeat protein alpha) plays a critical role in sorting hydrophobic parts of proteins that have become aberrantly exposed to the aqueous cytoplasm. These are thought to meet with one of three fates: targeting to a membrane, refolding by chaperones or degradation by the ubiquitin proteasome pathway. SGTA also stabilises steroid hormone receptors before they are activated for nuclear entry by their hormone ligands. I will show our recent findings on understanding this complex tweezer-like protein using a combination of methods.\u003C\/span\u003E\u003C\/span\u003E\u003C\/span\u003E\u003C\/p\u003E\r\n","summary":"","format":"limited_html"}],"field_subtitle":"","field_summary":[{"value":"\u003Cp\u003EOne in the series of SoCB seminars.\u003C\/p\u003E\r\n","format":"limited_html"}],"field_summary_sentence":[{"value":"School of Chemistry \u0026 Biochemistry -Seminar Series"}],"uid":"36441","created_gmt":"2024-01-09 16:03:44","changed_gmt":"2024-02-22 18:58:49","author":"slawrence67","boilerplate_text":"","field_publication":"","field_article_url":"","field_event_time":{"event_time_start":"2024-03-15T11:00:00-04:00","event_time_end":"2024-03-15T12:00:00-04:00","event_time_end_last":"2024-03-15T12:00:00-04:00","gmt_time_start":"2024-03-15 15:00:00","gmt_time_end":"2024-03-15 16:00:00","gmt_time_end_last":"2024-03-15 16:00:00","rrule":null,"timezone":"America\/New_York"},"location":"Children\u2019s Healthcare Seminar Room, EBB","extras":[],"groups":[{"id":"85951","name":"School of Chemistry and Biochemistry"}],"categories":[],"keywords":[],"core_research_areas":[],"news_room_topics":[],"event_categories":[{"id":"1795","name":"Seminar\/Lecture\/Colloquium"}],"invited_audience":[{"id":"78761","name":"Faculty\/Staff"},{"id":"174045","name":"Graduate students"},{"id":"177814","name":"Postdoc"},{"id":"78751","name":"Undergraduate students"}],"affiliations":[],"classification":[],"areas_of_expertise":[],"news_and_recent_appearances":[],"phone":[],"contact":[{"value":"\u003Cp\u003E\u003Cstrong\u003EHosts\u003C\/strong\u003E: Professors Racquel Lieberman \u0026amp; Lynn Kamerlin\u003C\/p\u003E\r\n","format":"limited_html"}],"email":[],"slides":[],"orientation":[],"userdata":""}}}