{"663007":{"#nid":"663007","#data":{"type":"event","title":"BioE PhD Proposal Presentation- Rachel Ringquist","body":[{"value":"\u003Cp\u003E\u003Cstrong\u003EAdvisor: \u003C\/strong\u003EKrishnendu Roy, PhD (Biomedical Engineering)\u003C\/p\u003E\r\n\r\n\u003Cp\u003E\u0026nbsp;\u003C\/p\u003E\r\n\r\n\u003Cp\u003E\u003Cstrong\u003ECommittee Members: \u003C\/strong\u003E\u003C\/p\u003E\r\n\r\n\u003Cp\u003EAnkur Singh, PhD (Mechanical Engineering)\u003C\/p\u003E\r\n\r\n\u003Cp\u003EHang Lu, PhD (Chemical and Biomolecular Engineering)\u003C\/p\u003E\r\n\r\n\u003Cp\u003EAhmet Coskun, PhD (Biomedical Engineering)\u003C\/p\u003E\r\n\r\n\u003Cp\u003ERabin Tirouvanzium, PhD (Department of Pediatrics, Emory University)\u003C\/p\u003E\r\n\r\n\u003Cp\u003E\u0026nbsp;\u003C\/p\u003E\r\n\r\n\u003Cp\u003E\u003Cstrong\u003EAn immune-competent microvascularized human lung-on-chip device for studying immunopathologies of the lung\u003C\/strong\u003E\u003C\/p\u003E\r\n\r\n\u003Cp\u003E\u0026nbsp;\u003C\/p\u003E\r\n\r\n\u003Cp\u003EAdvances in microphysiological organ-on-chip technologies have enabled spatiotemporal investigation into the complex physiology of organ systems in healthy and disease-like conditions \u003Cem\u003Ein vitro\u003C\/em\u003E. The highly-tunable nature of on-chip models permit direct manipulation of the microenvironment and provides the framework to study disease progression in ways not feasible through other \u003Cem\u003Ein vitro \u003C\/em\u003Emodels or through \u003Cem\u003Ein vivo \u003C\/em\u003Eanimal models. Organ-on-chip models encompass cellular heterogeneity and structural organization that mimics an \u003Cem\u003Ein vivo \u003C\/em\u003Eorgan microenvironment, while still allowing for real-time, cellular-level spatial and temporal analysis. While organ-on-chip systems are becoming increasingly popular, there remains a disconnect between \u003Cem\u003Ein vitro \u003C\/em\u003Emodels of the immune system and organ-on-chip models, with very few organ-on-chips incorporating immune components. Immune dysregulation is a hallmark of nearly all disease states and thus the ability to model immune signals \u003Cem\u003Ein vitro \u003C\/em\u003Eis paramount for the development of effective therapeutics.\u003C\/p\u003E\r\n\r\n\u003Cp\u003EWe aim to address this knowledge gap through the development of an immune-competent, fully microvascularized, microfluidic human lung-on-chip device. Our overall hypotheses are (1) incorporation of tissue-resident macrophages and circulating immune cells into a lung-on-chip model will enable the recapitulation of hallmark immune dysfunction in an influenza A (H1N1) infection model and (2) development of the human lung disease model will allow identification of key drivers of disease-specific immune dysregulation and illuminate potential immunomodulatory therapies. The proposed specific aims to test these hypotheses are to (1) develop an immune-competent lung-on-chip device with tissue-resident and circulating immune populations and (2) develop and characterize viral infection in a lung-on-chip model using H1N1-induced immune activation. To date, we have demonstrated the successful incorporation of tissue-resident macrophages and circulating immune cells into a microvascularized, human lung-on-chip device. Furthermore, we have evaluated the role of tissue-resident macrophages in the immune response to H1N1 infection. Future work aims to identify key circulating immune cells involved in the response to H1N1 infection and identify potential avenues for immunomodulatory therapies. The \u003Cem\u003Ein vitro \u003C\/em\u003Eimmune response will be fully characterized using single cell RNA sequencing, flow cytometry, multiplexed cytokine analysis, and spatial-omics techniques, and the resulting information will be used to inform treatment strategies.\u003C\/p\u003E\r\n","summary":null,"format":"limited_html"}],"field_subtitle":"","field_summary":[{"value":"\u003Cp\u003EBioE PhD Proposal Presentation- \u0026quot;An immune-competent microvascularized human lung-on-chip device for studying immunopathologies of the lung\u0026quot; - Rachel Ringquist\u003C\/p\u003E\r\n","format":"limited_html"}],"field_summary_sentence":[{"value":"\u0022An immune-competent microvascularized human lung-on-chip device for studying immunopathologies of the lung\u0022"}],"uid":"27917","created_gmt":"2022-11-08 16:20:13","changed_gmt":"2022-11-08 16:20:13","author":"Laura Paige","boilerplate_text":"","field_publication":"","field_article_url":"","field_event_time":{"event_time_start":"2022-11-14T15:30:00-05:00","event_time_end":"2022-11-14T17:30:00-05:00","event_time_end_last":"2022-11-14T17:30:00-05:00","gmt_time_start":"2022-11-14 20:30:00","gmt_time_end":"2022-11-14 22:30:00","gmt_time_end_last":"2022-11-14 22:30:00","rrule":null,"timezone":"America\/New_York"},"extras":[],"groups":[{"id":"65448","name":"Bioengineering Graduate Program"}],"categories":[],"keywords":[{"id":"172056","name":"go-BioE"}],"core_research_areas":[],"news_room_topics":[],"event_categories":[{"id":"10377","name":"Career\/Professional development"}],"invited_audience":[{"id":"78761","name":"Faculty\/Staff"},{"id":"78771","name":"Public"},{"id":"78751","name":"Undergraduate students"}],"affiliations":[],"classification":[],"areas_of_expertise":[],"news_and_recent_appearances":[],"phone":[],"contact":[],"email":[],"slides":[],"orientation":[],"userdata":""}}}