{"660361":{"#nid":"660361","#data":{"type":"event","title":"BioE PhD Defense Presentation- Karen Martin","body":[{"value":"\u003Cp\u003E\u003Cstrong\u003EAdvisor: \u003C\/strong\u003E\u003C\/p\u003E\r\n\r\n\u003Cp\u003EAndr\u0026eacute;s Garc\u0026iacute;a, PhD (Georgia Institute of Technology)\u003C\/p\u003E\r\n\r\n\u003Cp\u003E\u0026nbsp;\u003C\/p\u003E\r\n\r\n\u003Cp\u003E\u003Cstrong\u003ECommittee Members:\u003C\/strong\u003E\u003C\/p\u003E\r\n\r\n\u003Cp\u003EEdward Botchwey, PhD (Georgia Institute of Technology)\u003C\/p\u003E\r\n\r\n\u003Cp\u003ELevi Wood, PhD (Georgia Institute of Technology)\u003C\/p\u003E\r\n\r\n\u003Cp\u003ENick Willett, PhD (University of Oregon)\u003C\/p\u003E\r\n\r\n\u003Cp\u003EEsma Yolcu, PhD (University of Missouri)\u003C\/p\u003E\r\n\r\n\u003Cp\u003E\u0026nbsp;\u003C\/p\u003E\r\n\r\n\u003Cp\u003E\u003Cstrong\u003EHydrogel-delivery of mesenchymal stem cells to modulate the local immune environment and direct tissue repair\u003C\/strong\u003E\u003C\/p\u003E\r\n\r\n\u003Cp\u003E\u0026nbsp;\u003C\/p\u003E\r\n\r\n\u003Cp\u003EMesenchymal stem\/stromal cells (MSC) are actively being explored for use in a variety of regenerative medicine applications due to their potent immunosuppressive and anti-inflammatory properties. Traditionally, these cells have been delivered by bolus injection, but the need to prolong the survival and retention of MSC at sites of injury has spurred the development of a variety of biomaterial-based MSC delivery vehicles. Many studies have explored how biomaterial properties modulate MSC behaviors in both \u003Cem\u003Ein vitro\u003C\/em\u003E and \u003Cem\u003Ein vivo\u003C\/em\u003E contexts. However, a majority of the \u003Cem\u003Ein vivo\u003C\/em\u003E studies were carried out in immunocompromised mice, neglecting the key interaction of the host immune system with these biomaterial-MSC constructs. Additionally, while many properties of synthetic biomaterials are well-defined, controlling biomaterial degradation rates in \u003Cem\u003Ein vivo \u003C\/em\u003Eenvironments remains a significant, therapeutic-limiting challenge. The objective of this thesis is to utilize immunocompetent mouse models to evaluate the immunomodulatory and regenerative effects of engineered biomaterial-MSC constructs. In Aim 1, I demonstrate that subcutaneous delivery of murine MSC within synthetic hydrogels in immunocompetent mice modulates the local cytokine milieu and temporal recruitment of immune cells to the hydrogel. In Aim 2, I show that, when delivered subcutaneously in a hydrogel, the fetal bovine serum used for \u003Cem\u003Eex vivo\u003C\/em\u003E MSC expansion elicits a robust type 2 immune response characterized by infiltration of eosinophils and CD4\u003Csup\u003E+\u003C\/sup\u003E T cells and that this immune response impairs bone repair. Finally, in Aim 3, I utilize hydrolytically degradable ester linkage groups to engineer PEG hydrogels with tunable \u003Cem\u003Ein vivo\u003C\/em\u003E degradation kinetics for enhanced delivery of MSC to diabetic cutaneous wounds. Overall, this work yields critical insights into MSC-immune cell interactions \u003Cem\u003Ein vivo\u003C\/em\u003E and highlights strategies for modulating these interactions through the use of engineered biomaterial MSC delivery vehicles.\u003C\/p\u003E\r\n","summary":null,"format":"limited_html"}],"field_subtitle":"","field_summary":[{"value":"\u003Cp\u003EBioE PhD Defense Presentation- \u0026quot;Hydrogel-delivery of mesenchymal stem cells to modulate the local immune environment and direct tissue repair\u0026quot; - Karen Martin\u003C\/p\u003E\r\n","format":"limited_html"}],"field_summary_sentence":[{"value":"\u0022Hydrogel-delivery of mesenchymal stem cells to modulate the local immune environment and direct tissue repair\u0022"}],"uid":"27917","created_gmt":"2022-08-20 18:30:14","changed_gmt":"2022-08-20 18:30:14","author":"Laura Paige","boilerplate_text":"","field_publication":"","field_article_url":"","field_event_time":{"event_time_start":"2022-09-01T14:00:00-04:00","event_time_end":"2022-09-01T16:00:00-04:00","event_time_end_last":"2022-09-01T16:00:00-04:00","gmt_time_start":"2022-09-01 18:00:00","gmt_time_end":"2022-09-01 20:00:00","gmt_time_end_last":"2022-09-01 20:00:00","rrule":null,"timezone":"America\/New_York"},"extras":[],"groups":[{"id":"65448","name":"Bioengineering Graduate Program"}],"categories":[],"keywords":[{"id":"172056","name":"go-BioE"}],"core_research_areas":[],"news_room_topics":[],"event_categories":[{"id":"10377","name":"Career\/Professional development"}],"invited_audience":[{"id":"78761","name":"Faculty\/Staff"},{"id":"78771","name":"Public"},{"id":"78751","name":"Undergraduate students"}],"affiliations":[],"classification":[],"areas_of_expertise":[],"news_and_recent_appearances":[],"phone":[],"contact":[],"email":[],"slides":[],"orientation":[],"userdata":""}}}