{"659716":{"#nid":"659716","#data":{"type":"event","title":"PhD Defense by Jennifer Rattray","body":[{"value":"\u003Cp\u003EIn partial fulfillment of the requirements for the degree of\u003C\/p\u003E\r\n\r\n\u003Cp\u003E\u0026nbsp;\u003C\/p\u003E\r\n\r\n\u003Cp\u003EDoctor of Philosophy in Biology\u003C\/p\u003E\r\n\r\n\u003Cp\u003EIn the\u003C\/p\u003E\r\n\r\n\u003Cp\u003ESchool of Biological Sciences\u003C\/p\u003E\r\n\r\n\u003Cp\u003E\u0026nbsp;\u003C\/p\u003E\r\n\r\n\u003Cp\u003E\u003Cstrong\u003EJennifer Rattray\u003C\/strong\u003E\u003C\/p\u003E\r\n\r\n\u003Cp\u003E\u0026nbsp;\u003C\/p\u003E\r\n\r\n\u003Cp\u003EWill defend her dissertation:\u003C\/p\u003E\r\n\r\n\u003Cp\u003E\u0026nbsp;\u003C\/p\u003E\r\n\r\n\u003Cp\u003E\u003Cstrong\u003ECollective behavior and morphological complexity in \u003Cem\u003EPseudomonas aeruginosa\u003C\/em\u003E\u003C\/strong\u003E\u003C\/p\u003E\r\n\r\n\u003Cp\u003E\u0026nbsp;\u003C\/p\u003E\r\n\r\n\u003Cp\u003EWednesday, August 3\u003Csup\u003Erd\u003C\/sup\u003E, 2022\u003C\/p\u003E\r\n\r\n\u003Cp\u003E1 PM Eastern Time\u003C\/p\u003E\r\n\r\n\u003Cp\u003E\u0026nbsp;\u003C\/p\u003E\r\n\r\n\u003Cp\u003EIn-person: The Dissertation Defense Room, 4th floor of Price Gilbert Library in room 4222\u003Cbr \/\u003E\r\nVirtual Link: \u0026nbsp;\u003Ca href=\u0022https:\/\/gatech.zoom.us\/j\/93202356870\u0022 target=\u0022_blank\u0022\u003Ehttps:\/\/gatech.zoom.us\/j\/93202356870\u003C\/a\u003E\u003C\/p\u003E\r\n\r\n\u003Cp\u003E\u0026nbsp;\u003Cstrong\u003EThesis Advisor:\u003C\/strong\u003E\u003C\/p\u003E\r\n\r\n\u003Cp\u003ESam P. Brown, Ph.D.\u003C\/p\u003E\r\n\r\n\u003Cp\u003ESchool of Biological Sciences\u003C\/p\u003E\r\n\r\n\u003Cp\u003EGeorgia Institute of Technology\u003C\/p\u003E\r\n\r\n\u003Cp\u003E\u0026nbsp;\u003C\/p\u003E\r\n\r\n\u003Cp\u003E\u003Cstrong\u003ECommittee Members:\u003C\/strong\u003E\u003C\/p\u003E\r\n\r\n\u003Cp\u003EMarvin Whiteley, Ph.D.\u003C\/p\u003E\r\n\r\n\u003Cp\u003ESchool of Biological Sciences\u003C\/p\u003E\r\n\r\n\u003Cp\u003EGeorgia Institute of Technology\u003C\/p\u003E\r\n\r\n\u003Cp\u003E\u0026nbsp;\u003C\/p\u003E\r\n\r\n\u003Cp\u003EPeter Yunker, Ph.D.\u003C\/p\u003E\r\n\r\n\u003Cp\u003ESchool of Physics\u003C\/p\u003E\r\n\r\n\u003Cp\u003EGeorgia Institute of Technology\u003C\/p\u003E\r\n\r\n\u003Cp\u003E\u0026nbsp;\u003C\/p\u003E\r\n\r\n\u003Cp\u003ESteve Diggle, Ph.D.\u003C\/p\u003E\r\n\r\n\u003Cp\u003ESchool of Biological Sciences\u003C\/p\u003E\r\n\r\n\u003Cp\u003EGeorgia Institute of Technology\u003C\/p\u003E\r\n\r\n\u003Cp\u003E\u0026nbsp;\u003C\/p\u003E\r\n\r\n\u003Cp\u003EWill Ratcliff, Ph.D.\u003C\/p\u003E\r\n\r\n\u003Cp\u003ESchool of Biological Sciences\u003C\/p\u003E\r\n\r\n\u003Cp\u003EGeorgia Institute of Technology\u003C\/p\u003E\r\n\r\n\u003Cp\u003E\u0026nbsp;\u003C\/p\u003E\r\n\r\n\u003Cp\u003ESUMMARY:\u003C\/p\u003E\r\n\r\n\u003Cp\u003E\u0026nbsp;\u003C\/p\u003E\r\n\r\n\u003Cp\u003EIn nature, many animals are capable of performing complex behaviors without centralized coordination. A well-studied focus is collective motion in flocks of birds and shoals of fish, both of which are capable of changing collective behavior as a function of individuals responding to their local environment. Similarly, despite their microscopic individual size, groups of bacteria are capable of collectively responding to and restructuring their environment. In this thesis I focus on the gamma proteobacterium \u003Cem\u003EPseudomonas aeruginosa \u003C\/em\u003E(PA), a well-studied opportunistic pathogen that is known to engage in complex collective behaviors, often controlled by a form of cell-cell communication mediated by diffusible signal molecules called quorum sensing (QS).\u003C\/p\u003E\r\n\r\n\u003Cp\u003EFirst, I query the sensing capacity of QS, quantifying the ability to sense cell density by tracking QS-regulated secreted protease (\u003Cem\u003ElasB\u003C\/em\u003E) expression on the population and single-cell scale. We find that PA can deliver a graded behavioral response (or \u0026lsquo;reaction norm\u0026rsquo;) to fine-scale variation in population density and show that populations generate graded responses to environmental variation through shifts in the proportion of cells responding and the intensity of responses. Given this ability of PA to quantitatively respond to discrete density environments, we then ask how the molecular machinery of QS shapes the reaction norms to changing density, via signal synthase knockout and complementation experiments. We find that the wildtype reaction norm is robust to the addition of density-independent signal supplements and more broadly, that a positive reaction norm to density is robust to multiple combinations of gene deletion and density-independent signal supplementation.\u003C\/p\u003E\r\n\r\n\u003Cp\u003ESwitching from QS control of a single gene (\u003Cem\u003ElasB\u003C\/em\u003E), I turn to a complex multigenic and multicellular trait of microcolony growth. Using a collection of diverse environmental and clinical PA isolates, we develop a colony image library of 69 strains in four-fold replication. We then use a combination of image processing techniques to quantify colony morphology and complexity and find that, under common laboratory conditions, morphology and complexity form a robust, repeatable phenotype on the level of individual strains. Based on this replicable visual \u0026ldquo;fingerprint\u0026rdquo; per strain, we reasoned that colony image data could be used to classify previously unseen colony images to the strain level. Using a combination of transfer learning and data augmentation we trained a neural network to classify strains, resulting in high-level accuracy (94%). These results indicate that not only do PA strains have characteristic, replicable \u0026lsquo;fingerprints\u0026rsquo;, but also that these \u0026lsquo;fingerprints\u0026rsquo; are learnable and classifiable. These results could provide a basis for predicting other strain-dependent behaviors including virulence or antibiotic resistance.\u0026nbsp;\u003C\/p\u003E\r\n\r\n\u003Cp\u003EOverall, these results highlight that complex and heterogeneous single-cell behaviors can produce robust and consistent patterns on the collective scale of environmental sensing and colony growth.\u003C\/p\u003E\r\n","summary":null,"format":"limited_html"}],"field_subtitle":"","field_summary":"","field_summary_sentence":[{"value":"  Collective behavior and morphological complexity in Pseudomonas aeruginosa"}],"uid":"27707","created_gmt":"2022-07-28 14:11:09","changed_gmt":"2022-07-28 14:11:09","author":"Tatianna Richardson","boilerplate_text":"","field_publication":"","field_article_url":"","field_event_time":{"event_time_start":"2022-08-03T14:00:00-04:00","event_time_end":"2022-08-03T17:00:00-04:00","event_time_end_last":"2022-08-03T17:00:00-04:00","gmt_time_start":"2022-08-03 18:00:00","gmt_time_end":"2022-08-03 21:00:00","gmt_time_end_last":"2022-08-03 21:00:00","rrule":null,"timezone":"America\/New_York"},"extras":[],"groups":[{"id":"221981","name":"Graduate Studies"}],"categories":[],"keywords":[{"id":"100811","name":"Phd Defense"}],"core_research_areas":[],"news_room_topics":[],"event_categories":[{"id":"1788","name":"Other\/Miscellaneous"}],"invited_audience":[{"id":"78761","name":"Faculty\/Staff"},{"id":"78771","name":"Public"},{"id":"78751","name":"Undergraduate students"}],"affiliations":[],"classification":[],"areas_of_expertise":[],"news_and_recent_appearances":[],"phone":[],"contact":[],"email":[],"slides":[],"orientation":[],"userdata":""}}}