{"658596":{"#nid":"658596","#data":{"type":"event","title":"PhD Defense by Alli L. Gombolay","body":[{"value":"\u003Cp\u003EIn partial fulfillment of the requirements for the degree of\u003C\/p\u003E\r\n\r\n\u003Cp\u003EDoctor of Philosophy in Bioinformatics\u003C\/p\u003E\r\n\r\n\u003Cp\u003Ein the School of Biological Sciences\u003C\/p\u003E\r\n\r\n\u003Cp\u003E\u0026nbsp;\u003C\/p\u003E\r\n\r\n\u003Cp\u003E\u003Cstrong\u003EAlli L. Gombolay\u003C\/strong\u003E\u003Cbr \/\u003E\r\n\u003Cbr \/\u003E\r\nDefends her thesis:\u003C\/p\u003E\r\n\r\n\u003Cp\u003E\u003Cstrong\u003ECharacterization of Biological Signatures of Ribonucleotides Incorporated into DNA using the Ribose-Map Bioinformatics Toolkit\u003C\/strong\u003E\u003C\/p\u003E\r\n\r\n\u003Cp\u003E\u0026nbsp;\u003C\/p\u003E\r\n\r\n\u003Cp\u003EThursday, June 23, 2022\u0026nbsp;\u003Cbr \/\u003E\r\n11:00 am Eastern Time\u003C\/p\u003E\r\n\r\n\u003Cp\u003EZoom = \u003Ca href=\u0022https:\/\/gatech.zoom.us\/j\/92161044967\u0022\u003Ehttps:\/\/gatech.zoom.us\/j\/92161044967\u003C\/a\u003E\u003C\/p\u003E\r\n\r\n\u003Cp\u003E\u0026nbsp;\u003C\/p\u003E\r\n\r\n\u003Cp\u003EThesis advisor:\u003Cbr \/\u003E\r\nDr. Francesca Storici, School of Biological Sciences, Georgia Institute of Technology\u003C\/p\u003E\r\n\r\n\u003Cp\u003E\u003Cbr \/\u003E\r\nCommittee Members:\u003C\/p\u003E\r\n\r\n\u003Cp\u003EDr. I. King Jordan, School of Biological Sciences, Georgia Institute of Technology\u003C\/p\u003E\r\n\r\n\u003Cp\u003E\u0026nbsp;\u003C\/p\u003E\r\n\r\n\u003Cp\u003EDr. Mark Borodovsky, School of Biomedical Engineering and School of Computational Science and Engineering, Georgia Institute of Technology\u003C\/p\u003E\r\n\r\n\u003Cp\u003E\u0026nbsp;\u003C\/p\u003E\r\n\r\n\u003Cp\u003EDr. Fredrik Vannberg, Department of Biology, Georgia State University\u003C\/p\u003E\r\n\r\n\u003Cp\u003E\u0026nbsp;\u003C\/p\u003E\r\n\r\n\u003Cp\u003EDr. Soojin Yi, Department of Ecology, Evolution, and Marine Biology, University of California, Santa Barbara\u003C\/p\u003E\r\n\r\n\u003Cp\u003E\u0026nbsp;\u003C\/p\u003E\r\n\r\n\u003Cp\u003E\u003Cstrong\u003EAbstract\u003C\/strong\u003E\u003C\/p\u003E\r\n\r\n\u003Cp\u003E\u0026nbsp;\u003C\/p\u003E\r\n\r\n\u003Cp\u003EThe incorporation of ribonucleoside monophosphates (rNMPs) into DNA is one of the most frequently occurring errors during DNA synthesis. To maintain genome integrity, the ribonuclease (RNase) H enzymes efficiently remove rNMPs that are mistakenly incorporated into DNA during DNA replication or repair. However, if these enzymes fail to remove rNMPs from DNA, the 2\u0026rsquo;-hydroxyl group of the ribose sugar of rNMPs can attack the double-helix backbone of DNA, leading to genome instability, such as increased mutations, replication stress, single-strand breaks, double-strand breaks, and alterations in the structural and mechanical properties of DNA. Recently, five high-throughput rNMP sequencing techniques have been developed (ribose-seq, emRiboSeq, Alk-HydEn-seq, RHII-HydEn-seq, and Pu-seq) to map the locations of rNMPs in DNA to single-nucleotide resolution. Since the development of rNMP sequencing techniques is recent, the biological signatures of rNMP incorporation in DNA have yet to be thoroughly characterized. In addition, a standardized toolkit to characterize the biological signatures of rNMP incorporation in DNA is needed. To address this, I created the Ribose-Map bioinformatics toolkit. In addition, I applied Ribose-Map to characterize the biological signatures of rNMP incorporation in the DNA of different species, strains, and RNase H-relevant genotypes of yeast. This work serves as a foundational resource for the emerging field of rNMP mapping, leading to an improved understanding of the role of rNMPs in genome stability.\u003C\/p\u003E\r\n\r\n\u003Cp\u003E\u0026nbsp;\u003C\/p\u003E\r\n","summary":null,"format":"limited_html"}],"field_subtitle":"","field_summary":"","field_summary_sentence":[{"value":"Characterization of Biological Signatures of Ribonucleotides Incorporated into DNA using the Ribose-Map Bioinformatics Toolkit"}],"uid":"27707","created_gmt":"2022-05-31 15:46:53","changed_gmt":"2022-05-31 15:46:53","author":"Tatianna Richardson","boilerplate_text":"","field_publication":"","field_article_url":"","field_event_time":{"event_time_start":"2022-06-23T12:00:00-04:00","event_time_end":"2022-06-23T15:00:00-04:00","event_time_end_last":"2022-06-23T15:00:00-04:00","gmt_time_start":"2022-06-23 16:00:00","gmt_time_end":"2022-06-23 19:00:00","gmt_time_end_last":"2022-06-23 19:00:00","rrule":null,"timezone":"America\/New_York"},"extras":[],"groups":[{"id":"221981","name":"Graduate Studies"}],"categories":[],"keywords":[{"id":"100811","name":"Phd Defense"}],"core_research_areas":[],"news_room_topics":[],"event_categories":[{"id":"1788","name":"Other\/Miscellaneous"}],"invited_audience":[{"id":"78761","name":"Faculty\/Staff"},{"id":"78771","name":"Public"},{"id":"78751","name":"Undergraduate students"}],"affiliations":[],"classification":[],"areas_of_expertise":[],"news_and_recent_appearances":[],"phone":[],"contact":[],"email":[],"slides":[],"orientation":[],"userdata":""}}}