{"56099":{"#nid":"56099","#data":{"type":"event","title":"test","body":[{"value":"\u003Cp\u003Etest\u003C\/p\u003E","summary":null,"format":"limited_html"}],"field_subtitle":"","field_summary":[{"value":"test","format":"limited_html"}],"field_summary_sentence":[{"value":"test"}],"uid":"27213","created_gmt":"2010-05-20 11:44:40","changed_gmt":"2016-10-08 01:51:29","author":"Teri Nagel","boilerplate_text":"","field_publication":"","field_article_url":"","field_event_time":{"event_time_start":"2010-02-18T08:00:00-05:00","event_time_end":"2010-02-18T09:00:00-05:00","event_time_end_last":"2010-02-18T09:00:00-05:00","gmt_time_start":"2010-02-18 13:00:00","gmt_time_end":"2010-02-18 14:00:00","gmt_time_end_last":"2010-02-18 14:00:00","rrule":null,"timezone":"America\/New_York"},"extras":[],"groups":[{"id":"1221","name":"College of Design"},{"id":"1227","name":"School of Music"}],"categories":[],"keywords":[{"id":"383","name":"test"}],"core_research_areas":[],"news_room_topics":[],"event_categories":[],"invited_audience":[],"affiliations":[],"classification":[],"areas_of_expertise":[],"news_and_recent_appearances":[],"phone":[],"contact":[{"value":"\u003Cstrong\u003ECorissa Jones\u003C\/strong\u003E\u003Cbr \/\u003EMusic Department, College of Architecture\u003Cbr \/\u003E\u003Ca href=\u0022http:\/\/www.gatech.edu\/contact\/index.html?id=cj112\u0022\u003EContact Corissa Jones\u003C\/a\u003E\u003Cbr \/\u003E\u003Cstrong\u003E404-894-8949\u003C\/strong\u003E","format":"limited_html"}],"email":[],"slides":[],"orientation":[],"userdata":""}},"64688":{"#nid":"64688","#data":{"type":"event","title":"Using Force to Probe Bipolar Activation of Integrins and Signaling at Cell Surfaces","body":[{"value":"\u003Cp\u003EABSTRACT\u003Cbr \/\u003EUsing an ultrasensitive force probe and basic statistical principles, we have developed a novel method to assay both the kinetic rates at which adhesive ligands dissociate from cell receptors and the rates at which the receptors separate from the cytoskeleton in the course of single molecule pulling experiments. Using results from probe tests of ICAM-1 bonds to L 2 integrin on leukocytes, I will show that cell signaling not only regulates lifetimes and strengths of the outer-ligand adhesive bond but also lifetimes and strengths of cohesive integrin anchoring to the cell cytostructure. Whether triggered from outside or inside the cell, the results indicate that the mechanical linkage between integrin tail domains and the cytoskeleton is a key effector of integrin activation in cell signaling (e.g. analogous to the \u201cmechanical transmission\u201d in an automobile).\u003C\/p\u003E\u003Cp\u003E\u0026nbsp;\u003C\/p\u003E","summary":null,"format":"limited_html"}],"field_subtitle":"","field_summary":[{"value":"\u003Cp\u003EBIOE Seminar Series\u003C\/p\u003E\u003Cp\u003EEvan Evans, PhD - Professor, Department of Physics, Boston University\u003C\/p\u003E\u003Cp\u003E\u0022Using Force to Probe Bipolar Activation of Integrins and Signaling at Cell Surfaces\u0022\u003C\/p\u003E","format":"limited_html"}],"field_summary_sentence":[{"value":"Evan Evans, PhD - Boston University"}],"uid":"27195","created_gmt":"2011-03-01 10:14:51","changed_gmt":"2016-10-08 01:54:26","author":"Colly Mitchell","boilerplate_text":"","field_publication":"","field_article_url":"","field_event_time":{"event_time_start":"2011-03-29T15:00:00-04:00","event_time_end":"2011-03-29T16:00:00-04:00","event_time_end_last":"2011-03-29T16:00:00-04:00","gmt_time_start":"2011-03-29 19:00:00","gmt_time_end":"2011-03-29 20:00:00","gmt_time_end_last":"2011-03-29 20:00:00","rrule":null,"timezone":"America\/New_York"},"extras":[],"related_links":[{"url":"http:\/\/physics.bu.edu\/people\/show\/evanse","title":"Evans Profile"}],"groups":[{"id":"1292","name":"Parker H. Petit Institute for Bioengineering and Bioscience (IBB)"}],"categories":[],"keywords":[{"id":"11877","name":"BioE Seminar"},{"id":"12183","name":"evan evans"},{"id":"109","name":"Georgia Tech"},{"id":"248","name":"IBB"},{"id":"12184","name":"Using Force to Probe Bipolar Activation of Integrins and Signaling at Cell Surfaces"}],"core_research_areas":[],"news_room_topics":[],"event_categories":[{"id":"1795","name":"Seminar\/Lecture\/Colloquium"}],"invited_audience":[],"affiliations":[],"classification":[],"areas_of_expertise":[],"news_and_recent_appearances":[],"phone":[],"contact":[{"value":"\u003Cp\u003EJames Godard\u003C\/p\u003E\u003Cp\u003EFaculty host:\u0026nbsp; Cheng Zhu\u003C\/p\u003E","format":"limited_html"}],"email":[],"slides":[],"orientation":[],"userdata":""}},"64195":{"#nid":"64195","#data":{"type":"event","title":"Molecular Engineering and Live Cell Imaging for Studying Cell-Environment Interactions","body":[{"value":"\u003Cp\u003EAbstract:\n\u003C\/p\u003E\u003Cp\u003ESignaling molecules and their activities are well coordinated in\nspace and time to regulate cellular functions in response to mechanical\nand chemical microenvironment. Based on fluorescent resonance energy\ntransfer (FRET), we have developed several genetically encoded\nbiosensors for detecting the spatiotemporal activities of signaling\nmolecules, including Src, Rac1, MT1-MMP, and Calcium. A Rac biosensor\nrevealed that the Rac activity in cells constrained on micropatterned\nextracellular-matrix surface is polarized with higher activity\nconcentrated at the leading edge of migrating cells upon PDGF\nstimulation, whereas Src activities in these cells displayed global\nactivation patterns without obvious polarity. Our calcium biosensor\nalso allowed the revelation that there is a spontaneous Ca2+\noscillation in human mesenchymal stem cells (HMSCs) both inside the\ncytoplasm and endoplasmic reticulum (ER). The substrate stiffness where\nHMSCs are seeded can significantly affect this Ca2+ oscillation, in a\nfashion dependent on the RhoA signaling pathway. We have further\ndeveloped a FAK FRET biosensor and targeted it into lipid rafts or\nnon-rafts of plasma membrane by lipid modifications. Upon cell adhesion\non extracellular matrix proteins or stimulation by platelet-derived\ngrowth factor, the raft-targeting FAK biosensor showed a surprisingly\nstronger FRET response than that at non-rafts, suggesting that the FAK\nactivation mainly occurs at lipid rafts. Further experiments revealed\nthat the PDGF-induced FAK activation at rafts is mediated by the kinase\nactivity of Src, whereas FAK activation induced by adhesion is\nindependent of, and in fact essential for the Src activation. These\nresults suggest that FAK is activated at rafts with distinct activation\nmechanisms in response to different physiological stimuli. In summary,\nour novel FRET biosensors in combination with tools in\nnano-biotechnology and bio-photonics have made it possible to monitor\nkey signaling cascades in live cells with subcellular and dynamic\ncharacterization when cells interact with their physical\/chemical\nmicroenvironment.\u003C\/p\u003E","summary":null,"format":"limited_html"}],"field_subtitle":"","field_summary":[{"value":"\u003Cp\u003EBioengineering Seminar Series\u003C\/p\u003E\u003Cp\u003EYingxiao Wang, PhD - University of Illinois, Urbana-Champaign\n\u003Cem\u003E\u003C\/em\u003E\u003C\/p\u003E\u003Cp\u003E\u003Cem\u003EMolecular Engineering and Live Cell Imaging for Studying Cell-Environment Interactions\u003C\/em\u003E\u003C\/p\u003E","format":"limited_html"}],"field_summary_sentence":[{"value":"Yingxiao Wang, PhD - University of Illinois, Urbana-Champaign"}],"uid":"27195","created_gmt":"2011-02-11 10:48:59","changed_gmt":"2016-10-08 01:54:05","author":"Colly Mitchell","boilerplate_text":"","field_publication":"","field_article_url":"","field_event_time":{"event_time_start":"2011-03-01T10:00:00-05:00","event_time_end":"2011-03-01T11:00:00-05:00","event_time_end_last":"2011-03-01T11:00:00-05:00","gmt_time_start":"2011-03-01 15:00:00","gmt_time_end":"2011-03-01 16:00:00","gmt_time_end_last":"2011-03-01 16:00:00","rrule":null,"timezone":"America\/New_York"},"extras":[],"related_links":[{"url":"http:\/\/imaging.bioen.illinois.edu\/yingxiao_wang\/","title":"Wang Lab"}],"groups":[{"id":"1292","name":"Parker H. Petit Institute for Bioengineering and Bioscience (IBB)"}],"categories":[],"keywords":[{"id":"11877","name":"BioE Seminar"}],"core_research_areas":[],"news_room_topics":[],"event_categories":[{"id":"1795","name":"Seminar\/Lecture\/Colloquium"}],"invited_audience":[],"affiliations":[],"classification":[],"areas_of_expertise":[],"news_and_recent_appearances":[],"phone":[],"contact":[{"value":"\u003Cp\u003EFaculty Host: Andres Garcia 404-894-9384\u003C\/p\u003E","format":"limited_html"}],"email":[],"slides":[],"orientation":[],"userdata":""}}}