{"653858":{"#nid":"653858","#data":{"type":"event","title":"CMDI Seminar Series: Madeline Mei","body":[{"value":"\u003Cp\u003EBacteriocins are proteinaceous antimicrobials produced by bacteria, which are active against other strains of the same species. R-type pyocins are phage tail-like bacteriocins produced by \u003Cem\u003EPseudomonas aeruginosa\u003C\/em\u003E, a Gram-negative opportunistic pathogen known to be problematic in chronic infections. Due to their anti-pseudomonal activity, R-pyocins have potential as therapeutics in infection. However little is understood regarding the effects of cell lysis in infection, nor the specific timing of R-pyocin induction and release. Similar to prophages, R-pyocins are located on the chromosome and are induced by the SOS response via DNA-damaging agents. Following SOS activation, R-pyocins are produced and then released into the environment by lysis of the producing cells. While several DNA-damaging agents are known to induce R-pyocin activity \u0026ndash; including mitomycin C, ciprofloxacin, and hydrogen peroxide \u0026ndash; it is unknown if all agents induce R-pyocin production to the same magnitude or along the same temporal regime. The induction of R-pyocins by the SOS response in P. aeruginosa also suggests that there may be regulatory ties between pyocins and chromosomal prophages - also induced under the same response. However, the regulation of R-pyocins and prophage have yet to be disentangled. There are two more lytic systems in \u003Cem\u003EP. aeruginosa \u003C\/em\u003Ethat may also play a role in R-pyocin release: the Alp and the Cid systems, which have previously been ignored in much of the R-pyocin work thus far.\u0026nbsp;\u003C\/p\u003E\r\n\r\n\u003Cp\u003EUsing chromosomal transcriptional reporters, we have generated a number of strains to (i) quantify the regulatory activity of various R-pyocin genes, (ii) assess gene expression by disparate induction agents, (iii) establish a timeline of R-pyocin regulation, from induction to lysis, and (vi) disentangle the involvement of prophage and other lytic systems in the regulation of pyocins in \u003Cem\u003EP. aeruginosa. \u003C\/em\u003EOverall, our work provides a more cohesive picture of R-pyocin regulation in \u003Cem\u003EP. aeruginosa.\u0026nbsp;\u003C\/em\u003E\u003C\/p\u003E\r\n","summary":null,"format":"limited_html"}],"field_subtitle":"","field_summary":[{"value":"\u003Cp\u003E\u003Cstrong\u003EInvestigating the temporal dynamics of R-\u0026nbsp;pyocin regulation and release\u0026nbsp;in\u0026nbsp;\u003Cem\u003EPseudomonas aeruginosa\u003C\/em\u003E\u003C\/strong\u003E\u003C\/p\u003E\r\n","format":"limited_html"}],"field_summary_sentence":[{"value":"CMDI Seminar Series 2021"}],"uid":"35021","created_gmt":"2021-12-21 01:18:56","changed_gmt":"2021-12-21 01:18:56","author":"mavdonina3","boilerplate_text":"","field_publication":"","field_article_url":"","field_event_time":{"event_time_start":"2021-12-10T15:00:00-05:00","event_time_end":"2021-12-10T16:00:00-05:00","event_time_end_last":"2021-12-10T16:00:00-05:00","gmt_time_start":"2021-12-10 20:00:00","gmt_time_end":"2021-12-10 21:00:00","gmt_time_end_last":"2021-12-10 21:00:00","rrule":null,"timezone":"America\/New_York"},"extras":[],"groups":[],"categories":[],"keywords":[],"core_research_areas":[],"news_room_topics":[],"event_categories":[],"invited_audience":[{"id":"78761","name":"Faculty\/Staff"},{"id":"78771","name":"Public"},{"id":"78751","name":"Undergraduate students"}],"affiliations":[],"classification":[],"areas_of_expertise":[],"news_and_recent_appearances":[],"phone":[],"contact":[{"value":"\u003Cp\u003E\u0026nbsp;\u003C\/p\u003E\r\n\r\n\u003Cp\u003E\u0026nbsp;\u003C\/p\u003E\r\n\r\n\u003Cp\u003E\u0026nbsp;\u003C\/p\u003E\r\n\r\n\u003Cp\u003E\u0026nbsp;\u003C\/p\u003E\r\n","format":"limited_html"}],"email":[],"slides":[],"orientation":[],"userdata":""}}}