{"647259":{"#nid":"647259","#data":{"type":"event","title":"PhD Defense by Aaron Rosado","body":[{"value":"\u003Cp\u003E\u003Cstrong\u003EAaron Rosado\u003C\/strong\u003E\u003C\/p\u003E\r\n\r\n\u003Cp\u003E\u003Cstrong\u003EPhD Defense Presentation\u003C\/strong\u003E\u003C\/p\u003E\r\n\r\n\u003Cp\u003E\u0026nbsp;\u003C\/p\u003E\r\n\r\n\u003Cp\u003E\u003Cstrong\u003EDate:\u003C\/strong\u003E Tuesday, May 18\u003Csup\u003Eth\u003C\/sup\u003E, 2021\u003C\/p\u003E\r\n\r\n\u003Cp\u003E\u003Cstrong\u003ETime:\u003C\/strong\u003E 10:00 AM\u003C\/p\u003E\r\n\r\n\u003Cp\u003E\u003Cstrong\u003ELocation:\u003C\/strong\u003E \u003Ca href=\u0022https:\/\/bluejeans.com\/743201883\u0022\u003Ehttps:\/\/bluejeans.com\/743201883\u003C\/a\u003E\u003C\/p\u003E\r\n\r\n\u003Cp\u003E\u0026nbsp;\u003C\/p\u003E\r\n\r\n\u003Cp\u003E\u0026nbsp;\u003C\/p\u003E\r\n\r\n\u003Cp\u003E\u0026nbsp;\u003C\/p\u003E\r\n\r\n\u003Cp\u003E\u003Cstrong\u003EThesis Advisor: \u003C\/strong\u003E\u003C\/p\u003E\r\n\r\n\u003Cp\u003ECheng Zhu, PhD\u003C\/p\u003E\r\n\r\n\u003Cp\u003EGeorgia Institute of Technology, WH Coulter Department of Biomedical Engineering\u003C\/p\u003E\r\n\r\n\u003Cp\u003E\u0026nbsp;\u003C\/p\u003E\r\n\r\n\u003Cp\u003E\u003Cstrong\u003EThesis Committee: \u003C\/strong\u003E\u003C\/p\u003E\r\n\r\n\u003Cp\u003EMichael Davis, PhD\u003C\/p\u003E\r\n\r\n\u003Cp\u003EGeorgia Institute of Technology, WH Coulter Department of Biomedical Engineering\u003C\/p\u003E\r\n\r\n\u003Cp\u003ESusan Thomas, PhD\u003C\/p\u003E\r\n\r\n\u003Cp\u003EGeorgia Institute of Technology, WH Coulter Department of Biomedical Engineering\u003C\/p\u003E\r\n\r\n\u003Cp\u003EArash Grakoui, PhD\u003C\/p\u003E\r\n\r\n\u003Cp\u003EEmory University School of Medicine, Department of Medicine, Division of Infectious Diseases\u003C\/p\u003E\r\n\r\n\u003Cp\u003EMichelle Krogsgaard, PhD\u003C\/p\u003E\r\n\r\n\u003Cp\u003ENYU School of Medicine, Department of Pathology\u003C\/p\u003E\r\n\r\n\u003Cp\u003E\u0026nbsp;\u003C\/p\u003E\r\n\r\n\u003Cp\u003E\u003Cstrong\u003ETitle:\u003C\/strong\u003E CD28 and TCR \u003Cem\u003EIn-situ\u003C\/em\u003E Biophysical Analyses Inform T Cell Immunity Mechanisms\u003C\/p\u003E\r\n\r\n\u003Cp\u003E\u0026nbsp;\u003C\/p\u003E\r\n\r\n\u003Cp\u003E\u003Cstrong\u003EAbstract:\u003C\/strong\u003E\u003C\/p\u003E\r\n\r\n\u003Cp\u003EThis work investigates two receptors on T lymphocytes that shape immunity, the T cell receptor (TCR) and cluster of differentiation 28 (CD28). T cells coordinate adaptive immunity, but how signaling via TCR and CD28 interactions with peptide-major compatibility complex (pMHC) and B7 family ligands on antigen presenting cells govern T cell function and differentiation remains poorly understood.\u0026nbsp; \u003Cem\u003EIn-situ\u003C\/em\u003E biophysical measurements on live T cell surfaces suggest both B7 family ligands form monomeric bonds with CD28. This work demonstrated CD28 catch bonds with B7 family ligands for the first time. Catch bonds refer to a counter-intuitive phenomenon where force prolongs bond lifetime contrasted with the more intuitive slip bond where force shortens bond lifetime.\u0026nbsp; Although TCR\u0026ndash;pMHC catch bonds on splenic T cells characterize a well-established TCR mechanosensing mechanism, the same interaction on hepatic T cells showed slip bonds correlating with a more activated state among liver T cells.\u0026nbsp; We also analyzed both short- and long-term memory effects from the same molecular interactions.\u0026nbsp; Short-term (within seconds) memory analyses found that bond formation increased bond formation likelihood but not dissociation in the immediate future.\u0026nbsp; Long-term (~5 minutes) memory analyses found that splenic T cells became more activated by repeated ligand engagement and receptor tension resulting in TCR\u0026ndash;pMHC catch bond elimination.\u0026nbsp; Our sensitive assay also revealed subtle T cell activation by piconewton-level T cell pushing and pulling forces as well as changes in short-term memory.\u0026nbsp; This work suggests biophysical instrumentation employed \u003Cem\u003Ein-situ\u003C\/em\u003E can reveal information about dynamic processes mediating important immunological functions.\u0026nbsp; The findings within this work provide insights into mechanistically how co-stimulation works at a single molecule level as well as how signaling overlap between TCR and CD28 influence receptor localization, mechanosensing, and triggering.\u0026nbsp; These insights answer longstanding mechanistic questions about how T cells function and provide foundations for future investigations.\u003C\/p\u003E\r\n\r\n\u003Cp\u003E\u0026nbsp;\u003C\/p\u003E\r\n","summary":null,"format":"limited_html"}],"field_subtitle":"","field_summary":"","field_summary_sentence":[{"value":"CD28 and TCR In-situ Biophysical Analyses Inform T Cell Immunity Mechanisms"}],"uid":"27707","created_gmt":"2021-05-07 14:43:54","changed_gmt":"2021-05-07 14:43:54","author":"Tatianna Richardson","boilerplate_text":"","field_publication":"","field_article_url":"","field_event_time":{"event_time_start":"2021-05-18T11:00:00-04:00","event_time_end":"2021-05-18T13:00:00-04:00","event_time_end_last":"2021-05-18T13:00:00-04:00","gmt_time_start":"2021-05-18 15:00:00","gmt_time_end":"2021-05-18 17:00:00","gmt_time_end_last":"2021-05-18 17:00:00","rrule":null,"timezone":"America\/New_York"},"extras":[],"groups":[{"id":"221981","name":"Graduate Studies"}],"categories":[],"keywords":[{"id":"100811","name":"Phd Defense"}],"core_research_areas":[],"news_room_topics":[],"event_categories":[{"id":"1788","name":"Other\/Miscellaneous"}],"invited_audience":[{"id":"78761","name":"Faculty\/Staff"},{"id":"78771","name":"Public"},{"id":"174045","name":"Graduate students"},{"id":"78751","name":"Undergraduate students"}],"affiliations":[],"classification":[],"areas_of_expertise":[],"news_and_recent_appearances":[],"phone":[],"contact":[],"email":[],"slides":[],"orientation":[],"userdata":""}}}