{"64716":{"#nid":"64716","#data":{"type":"news","title":"Researchers Predict Age of T Cells to Improve Cancer Treatment","body":[{"value":"\u003Cp\u003EManipulation of cells by a new microfluidic device may help clinicians improve a promising cancer therapy that harnesses the body\u0027s own immune cells to fight such diseases as metastatic melanoma, non-Hodgkin\u0027s lymphoma, chronic lymphocytic leukemia and neuroblastoma.\u003C\/p\u003E\n\u003Cp\u003EThe therapy, known as adoptive T cell transfer, has shown encouraging results in clinical trials. This treatment involves removing disease-fighting immune cells called T cells from a cancer patient, multiplying them in the laboratory and then infusing them back into the patient\u0027s body to attack the cancer. The effectiveness of this therapy, however, is limited by the finite lifespan of T cells -- after many divisions, these cells become unresponsive and inactive.\n\u003C\/p\u003E\n\u003Cp\u003EResearchers at Georgia Tech and Emory University have addressed this limitation by developing a microfluidic device for sample handling that allows a statistical model to be generated to evaluate cell responsiveness and accurately predict cell \u0022age\u0022 and quality. Being able to assess the age and responsiveness of T cells -- and therefore transfer only young functional cells back into a cancer patient\u0027s body -- offers the potential to improve the therapeutic outcome of several cancers.\n\u003C\/p\u003E\n\u003Cp\u003E\u0022The statistical model, enabled by the data generated with the microfluidic device, revealed an optimal combination of extracellular and intracellular proteins that accurately predict T cell age,\u0022 said Melissa Kemp, an assistant professor in the Wallace H. Coulter Department of Biomedical Engineering at Georgia Tech and Emory University. \u0022Knowing this information will help facilitate the clinical development of appropriate T cell expansion and selection protocols.\u0022 \n\u003C\/p\u003E\n\u003Cp\u003EDetails on the microfluidic device and statistical model were published in the March issue of the journal \u003Cem\u003EMolecular \u0026amp; Cellular Proteomics\u003C\/em\u003E. This work was supported by the National Institutes of Health, Georgia Cancer Coalition, and Georgia Tech Integrative Biosystems Institute.\u003C\/p\u003E\n\u003Cp\u003ECurrently, clinicians measure T cell age by using multiple assays that rely on measurements from large cell populations. The measurements determine if cells are exhibiting functions known to appear at different stages in the life cycle of a T cell.\n\u003C\/p\u003E\n\u003Cp\u003E\u0022Since no one measurement is a perfect predictor, it is advantageous to concurrently sample multiple proteins at different time points, which we can do with our microfluidic device,\u0022 explained Kemp, who is also a Georgia Cancer Coalition Distinguished Professor. \u0022The wealth of information we get from our device for a small number of cells far exceeds a single measurement from a population the same size by another assay type.\u0022\n\u003C\/p\u003E\n\u003Cp\u003EFor their study, Kemp, electrical engineering graduate student Catherine Rivet and biomedical engineering undergraduate student Abby Hill analyzed CD8+ T cells from healthy blood donors. They acquired information from 25 static biomarkers and 48 dynamic signaling measurements and found a combination of phenotypic markers and protein signaling dynamics -- including Lck, ERK, CD28 and CD27 -- to be the most useful in predicting cellular age.\n\u003C\/p\u003E\n\u003Cp\u003ETo obtain biomarker and dynamic signaling event measurements, the researchers ran the donor T cells through a microfluidic device designed in collaboration with Hang Lu, an associate professor in the Georgia Tech School of Chemical \u0026amp; Biomolecular Engineering. After stimulating the cells, the device divided them into different channels corresponding to eight different time points, ranging from 30 seconds to seven minutes. Then they were divided again into populations that were chemically treated to halt the biochemical reactions at snapshots in time to build up a picture of the signaling events that occurred as the T cells responded to antigen.\u003C\/p\u003E\n\u003Cp\u003E\u0022While donor-to-donor variability is a confounding factor in these types of experiments, the technological platform minimized the experimental data variance and allowed stimulation time to be precisely controlled,\u0022 said Lu.\n\u003C\/p\u003E\n\u003Cp\u003EWith the donor T cell data, the researchers developed a model to assess which biomarkers or dynamical signaling events best predicted the quality of T cell function. The model found the most informative data in predicting cellular age to be the initial changes in signaling dynamics.\n\u003C\/p\u003E\n\u003Cp\u003E\u0022Although a combination of biomarker and dynamic signaling data provided the optimal model, our results suggest that signaling information alone can predict cellular age almost as well as the entire dataset,\u0022 noted Kemp. \n\u003C\/p\u003E\n\u003Cp\u003EIn the future, Kemp plans to use this approach of combining multiple cell-based experiments on a microfluidic chip to integrate single-cell information with population-averaged techniques, such as multiplexed immunoassays or mass spectrometry.\n\u003C\/p\u003E\n\u003Cp\u003E\u003Cem\u003EThis project is supported in part by the National Institutes of Health (NIH)(Grant No. R21CA134299). The content is solely the responsibility of the principal investigator and does not necessarily represent the official views of the NIH.\u003C\/em\u003E\n\u003C\/p\u003E\n\u003Cp\u003E\u003Cstrong\u003EResearch News \u0026amp; Publications Office\u003Cbr \/\u003E\nGeorgia Institute of Technology\u003Cbr \/\u003E\n75 Fifth Street, N.W., Suite 314\u003Cbr \/\u003E\nAtlanta, Georgia  30308  USA\u003C\/strong\u003E\n\u003C\/p\u003E\n\u003Cp\u003E\u003Cstrong\u003EMedia Relations Contacts:\u003C\/strong\u003E Abby Robinson (abby@innovate.gatech.edu; 404-385-3364) or John Toon (jtoon@gatech.edu; 404-894-6986)\n\u003C\/p\u003E\n\u003Cp\u003E\u003Cstrong\u003EWriter:\u003C\/strong\u003E Abby Robinson\u003C\/p\u003E","summary":null,"format":"limited_html"}],"field_subtitle":"","field_summary":[{"value":"\u003Cp\u003EResearchers are accurately predicting T cell age and quality in order to improve the effectiveness of the cancer therapy known as adoptive T cell transfer, which is currently limited by the cells\u0027 finite lifespan.\u003C\/p\u003E","format":"limited_html"}],"field_summary_sentence":[{"value":"Predicting age of T cells could improve cancer therapy"}],"uid":"27206","created_gmt":"2011-03-02 01:00:00","changed_gmt":"2016-10-08 03:08:18","author":"Abby Vogel Robinson","boilerplate_text":"","field_publication":"","field_article_url":"","dateline":{"date":"2011-03-02T00:00:00-05:00","iso_date":"2011-03-02T00:00:00-05:00","tz":"America\/New_York"},"extras":[],"hg_media":{"64717":{"id":"64717","type":"image","title":"Catherine Rivet, Abby Hill and Melissa Kemp","body":null,"created":"1449176765","gmt_created":"2015-12-03 21:06:05","changed":"1475894569","gmt_changed":"2016-10-08 02:42:49","alt":"Catherine Rivet, Abby Hill and Melissa Kemp","file":{"fid":"192077","name":"tti74257.jpg","image_path":"\/sites\/default\/files\/images\/tti74257_0.jpg","image_full_path":"http:\/\/hg.gatech.edu\/\/sites\/default\/files\/images\/tti74257_0.jpg","mime":"image\/jpeg","size":1333865,"path_740":"http:\/\/hg.gatech.edu\/sites\/default\/files\/styles\/740xx_scale\/public\/images\/tti74257_0.jpg?itok=3n28yRUc"}},"64718":{"id":"64718","type":"image","title":"Melissa Kemp","body":null,"created":"1449176765","gmt_created":"2015-12-03 21:06:05","changed":"1475894569","gmt_changed":"2016-10-08 02:42:49","alt":"Melissa Kemp","file":{"fid":"192078","name":"tbp74257.jpg","image_path":"\/sites\/default\/files\/images\/tbp74257_0.jpg","image_full_path":"http:\/\/hg.gatech.edu\/\/sites\/default\/files\/images\/tbp74257_0.jpg","mime":"image\/jpeg","size":1153544,"path_740":"http:\/\/hg.gatech.edu\/sites\/default\/files\/styles\/740xx_scale\/public\/images\/tbp74257_0.jpg?itok=iK0CuVDW"}},"64719":{"id":"64719","type":"image","title":"Microfluidic device","body":null,"created":"1449176765","gmt_created":"2015-12-03 21:06:05","changed":"1475894569","gmt_changed":"2016-10-08 02:42:49","alt":"Microfluidic device","file":{"fid":"192079","name":"tfd74257.jpg","image_path":"\/sites\/default\/files\/images\/tfd74257_0.jpg","image_full_path":"http:\/\/hg.gatech.edu\/\/sites\/default\/files\/images\/tfd74257_0.jpg","mime":"image\/jpeg","size":1055020,"path_740":"http:\/\/hg.gatech.edu\/sites\/default\/files\/styles\/740xx_scale\/public\/images\/tfd74257_0.jpg?itok=JYMLVnWt"}}},"media_ids":["64717","64718","64719"],"related_links":[{"url":"http:\/\/www.bme.gatech.edu\/facultystaff\/faculty_record.php?id=97","title":"Melissa Kemp"},{"url":"http:\/\/www.chbe.gatech.edu\/faculty\/lu.php","title":"Hang Lu"},{"url":"http:\/\/dx.doi.org\/10.1074\/mcp.M110.003921","title":"Molecular \u0026 Cellular Proteomics paper"},{"url":"http:\/\/www.bme.gatech.edu\/","title":"Wallace H. Coulter Department of Biomedical Engineering"},{"url":"http:\/\/www.chbe.gatech.edu\/","title":"School of Chemical \u0026 Biomolecular Engineering"}],"groups":[{"id":"1188","name":"Research Horizons"}],"categories":[{"id":"140","name":"Cancer Research"},{"id":"141","name":"Chemistry and Chemical Engineering"},{"id":"145","name":"Engineering"},{"id":"146","name":"Life Sciences and Biology"},{"id":"135","name":"Research"}],"keywords":[{"id":"12210","name":"Adoptive Immunotherapy"},{"id":"12211","name":"adoptive t cell transfer"},{"id":"7214","name":"biomarker"},{"id":"249","name":"Biomedical Engineering"},{"id":"1704","name":"chemical \u0026 biomolecular engineering"},{"id":"12214","name":"Chronic Lymphocytic Leukemia"},{"id":"594","name":"college of engineering"},{"id":"898","name":"Hang Lu"},{"id":"4514","name":"immunotherapy"},{"id":"5084","name":"Melissa Kemp"},{"id":"12212","name":"Metastatic Melanoma"},{"id":"12216","name":"Microfluidic Device"},{"id":"12215","name":"Neuroblastoma"},{"id":"12213","name":"non-Hodgkin\u2019s lymphoma"},{"id":"9047","name":"T cell"},{"id":"12217","name":"t cell age"},{"id":"12218","name":"T cell Assays"},{"id":"12209","name":"t cell therapy"}],"core_research_areas":[],"news_room_topics":[],"event_categories":[],"invited_audience":[],"affiliations":[],"classification":[],"areas_of_expertise":[],"news_and_recent_appearances":[],"phone":[],"contact":[{"value":"\u003Cp\u003E\u003Cstrong\u003EAbby Robinson\u003C\/strong\u003E\u003Cbr \/\u003EResearch News and Publications\u003Cbr \/\u003E\u003Ca href=\u0022http:\/\/www.gatech.edu\/contact\/index.html?id=avogel6\u0022\u003EContact Abby Robinson\u003C\/a\u003E\u003Cbr \/\u003E\u003Cstrong\u003E404-385-3364\u003C\/strong\u003E\u003C\/p\u003E","format":"limited_html"}],"email":["abby@innovate.gatech.edu"],"slides":[],"orientation":[],"userdata":""}}}