{"642946":{"#nid":"642946","#data":{"type":"event","title":"PhD Proposal by Alejandro J. Da Silva Sanchez ","body":[{"value":"\u003Cp\u003E\u003Cstrong\u003EBioE PhD Thesis Proposal Presentation\u003C\/strong\u003E\u003C\/p\u003E\r\n\r\n\u003Cp\u003E\u003Cstrong\u003EAlejandro J. Da Silva Sanchez\u0026nbsp;\u003C\/strong\u003E\u003C\/p\u003E\r\n\r\n\u003Cp\u003E\u0026nbsp;\u003C\/p\u003E\r\n\r\n\u003Cp\u003EJanuary 19\u003Csup\u003Eth\u003C\/sup\u003E, 2021, 3:00 PM\u0026nbsp;\u003C\/p\u003E\r\n\r\n\u003Cp\u003ELink:\u0026nbsp;\u003Ca href=\u0022https:\/\/nam12.safelinks.protection.outlook.com\/?url=https%3A%2F%2Fbluejeans.com%2F425098357\u0026amp;data=04%7C01%7Ctatianna.richardson%40grad.gatech.edu%7C2ca55c348f6b43e470e608d8b8bdba13%7C482198bbae7b4b258b7a6d7f32faa083%7C0%7C0%7C637462472295311962%7CUnknown%7CTWFpbGZsb3d8eyJWIjoiMC4wLjAwMDAiLCJQIjoiV2luMzIiLCJBTiI6Ik1haWwiLCJXVCI6Mn0%3D%7C1000\u0026amp;sdata=On2Hp0gwvmw4buyCFFkl5We5RwksK0mM45GsSqT1D9Y%3D\u0026amp;reserved=0\u0022 target=\u0022_blank\u0022 title=\u0022Original URL: https:\/\/bluejeans.com\/425098357. Click or tap if you trust this link.\u0022\u003Ehttps:\/\/bluejeans.com\/425098357\u003C\/a\u003E\u0026nbsp;\u003C\/p\u003E\r\n\r\n\u003Cp\u003E\u0026nbsp;\u003C\/p\u003E\r\n\r\n\u003Cp\u003E\u003Cstrong\u003EAdvisor:\u003C\/strong\u003E\u003C\/p\u003E\r\n\r\n\u003Cp\u003EJames E. Dahlman, Ph.D. BME, Georgia Institute of Technology and Emory University\u0026nbsp;\u003C\/p\u003E\r\n\r\n\u003Cp\u003E\u0026nbsp;\u003C\/p\u003E\r\n\r\n\u003Cp\u003E\u003Cstrong\u003ECommittee Members:\u0026nbsp;\u003C\/strong\u003E\u003C\/p\u003E\r\n\r\n\u003Cp\u003E\u0026nbsp;\u003C\/p\u003E\r\n\r\n\u003Cp\u003EPhilip J. Santangelo, Ph.D.\u0026nbsp;\u003C\/p\u003E\r\n\r\n\u003Cp\u003EBME, Georgia Institute of Technology and Emory University\u0026nbsp;\u003C\/p\u003E\r\n\r\n\u003Cp\u003E\u0026nbsp;\u003C\/p\u003E\r\n\r\n\u003Cp\u003EJulie A. Champion, Ph.D.\u0026nbsp;\u003C\/p\u003E\r\n\r\n\u003Cp\u003EChBE, Georgia Institute of Technology\u0026nbsp;\u003C\/p\u003E\r\n\r\n\u003Cp\u003E\u0026nbsp;\u003C\/p\u003E\r\n\r\n\u003Cp\u003EMark P. Styczynski, Ph.D.\u0026nbsp;\u003C\/p\u003E\r\n\r\n\u003Cp\u003EChBE, Georgia Institute of Technology\u0026nbsp;\u003C\/p\u003E\r\n\r\n\u003Cp\u003E\u0026nbsp;\u003C\/p\u003E\r\n\r\n\u003Cp\u003EMG Finn, Ph.D.\u0026nbsp;\u003C\/p\u003E\r\n\r\n\u003Cp\u003EChemistry and Biochemistry, Georgia Institute of Technology\u003C\/p\u003E\r\n\r\n\u003Cp\u003E\u0026nbsp;\u003C\/p\u003E\r\n\r\n\u003Cp\u003E\u0026nbsp;\u003C\/p\u003E\r\n\r\n\u003Cp\u003E\u003Cstrong\u003EThe impact of the metabolic state of a cell on nucleic acid therapeutics\u0026nbsp;\u003C\/strong\u003E\u003C\/p\u003E\r\n\r\n\u003Cp\u003E\u0026nbsp;\u003C\/p\u003E\r\n\r\n\u003Cp\u003ENucleic acid therapies have advanced over the last decade with the FDA approval of the first siRNA drug in 2018 and the recent approval of COVID vaccines leveraging mRNA technology. While surface receptors and endocytosis genes have been shown to influence the effectiveness of RNA drug delivery with lipid nanoparticles (LNPs), the effect of the metabolic state of a cell upon therapies seeking to produce or silence proteins remains understudied. This project, therefore, aims to (i) understand whether metabolic perturbations to the mTOR pathway upon PIP3 extracellular administration affect LNP-mediated mRNA delivery, (ii) develop cell- and mouse-agnostic high throughput LNP screening systems for siRNA and DNA drugs that will allow scientists to perform mechanistic studies on functional delivery with genetic knockout mice, and (iii) leverage these platforms to study whether mice exhibiting different levels of activity across the mTOR signaling pathway are more or less receptive to different nucleic acid drugs. This work will constitute early steps toward two equally important goals: (a) exploiting natural differences in cell signaling to improve cell-type-specific nanoparticle delivery and (b) understanding how different physiological states can lead to different delivery potencies of nucleic acid therapeutics.\u0026nbsp;\u003C\/p\u003E\r\n","summary":null,"format":"limited_html"}],"field_subtitle":"","field_summary":"","field_summary_sentence":[{"value":" The impact of the metabolic state of a cell on nucleic acid therapeutics "}],"uid":"27707","created_gmt":"2021-01-14 19:17:49","changed_gmt":"2021-01-14 19:17:49","author":"Tatianna Richardson","boilerplate_text":"","field_publication":"","field_article_url":"","field_event_time":{"event_time_start":"2021-01-19T15:00:00-05:00","event_time_end":"2021-01-19T17:00:00-05:00","event_time_end_last":"2021-01-19T17:00:00-05:00","gmt_time_start":"2021-01-19 20:00:00","gmt_time_end":"2021-01-19 22:00:00","gmt_time_end_last":"2021-01-19 22:00:00","rrule":null,"timezone":"America\/New_York"},"extras":[],"groups":[{"id":"221981","name":"Graduate Studies"}],"categories":[],"keywords":[{"id":"102851","name":"Phd proposal"}],"core_research_areas":[],"news_room_topics":[],"event_categories":[{"id":"1788","name":"Other\/Miscellaneous"}],"invited_audience":[{"id":"78761","name":"Faculty\/Staff"},{"id":"78771","name":"Public"},{"id":"174045","name":"Graduate students"},{"id":"78751","name":"Undergraduate students"}],"affiliations":[],"classification":[],"areas_of_expertise":[],"news_and_recent_appearances":[],"phone":[],"contact":[],"email":[],"slides":[],"orientation":[],"userdata":""}}}