{"642654":{"#nid":"642654","#data":{"type":"event","title":"BioE PhD Proposal Presentation- Alejandro J. Da Silva Sanchez ","body":[{"value":"\u003Cp\u003E\u003Cstrong\u003EAdvisor:\u003C\/strong\u003E\u003C\/p\u003E\r\n\r\n\u003Cp\u003EJames E. Dahlman, Ph.D. BME, Georgia Institute of Technology and Emory University\u0026nbsp;\u003C\/p\u003E\r\n\r\n\u003Cp\u003E\u0026nbsp;\u003C\/p\u003E\r\n\r\n\u003Cp\u003E\u003Cstrong\u003ECommittee Members:\u0026nbsp;\u003C\/strong\u003E\u003C\/p\u003E\r\n\r\n\u003Cp\u003E\u0026nbsp;\u003C\/p\u003E\r\n\r\n\u003Cp\u003EPhilip J. Santangelo, Ph.D.\u0026nbsp;\u003C\/p\u003E\r\n\r\n\u003Cp\u003EBME, Georgia Institute of Technology and Emory University\u0026nbsp;\u003C\/p\u003E\r\n\r\n\u003Cp\u003E\u0026nbsp;\u003C\/p\u003E\r\n\r\n\u003Cp\u003EJulie A. Champion, Ph.D.\u0026nbsp;\u003C\/p\u003E\r\n\r\n\u003Cp\u003EChBE, Georgia Institute of Technology\u0026nbsp;\u003C\/p\u003E\r\n\r\n\u003Cp\u003E\u0026nbsp;\u003C\/p\u003E\r\n\r\n\u003Cp\u003EMark P. Styczynski, Ph.D.\u0026nbsp;\u003C\/p\u003E\r\n\r\n\u003Cp\u003EChBE, Georgia Institute of Technology\u0026nbsp;\u003C\/p\u003E\r\n\r\n\u003Cp\u003E\u0026nbsp;\u003C\/p\u003E\r\n\r\n\u003Cp\u003EMG Finn, Ph.D.\u0026nbsp;\u003C\/p\u003E\r\n\r\n\u003Cp\u003EChemistry and Biochemistry, Georgia Institute of Technology\u003C\/p\u003E\r\n\r\n\u003Cp\u003E\u0026nbsp;\u003C\/p\u003E\r\n\r\n\u003Cp\u003E\u0026nbsp;\u003C\/p\u003E\r\n\r\n\u003Cp\u003E\u0026nbsp;\u003C\/p\u003E\r\n\r\n\u003Cp\u003E\u003Cstrong\u003EThe impact of the metabolic state of a cell on nucleic acid therapeutics\u0026nbsp;\u003C\/strong\u003E\u003C\/p\u003E\r\n\r\n\u003Cp\u003E\u0026nbsp;\u003C\/p\u003E\r\n\r\n\u003Cp\u003ENucleic acid therapies have advanced over the last decade with the FDA approval of the first siRNA drug in 2018 and the recent approval of COVID vaccines leveraging mRNA technology. While surface receptors and endocytosis genes have been shown to influence the effectiveness of RNA drug delivery with lipid nanoparticles (LNPs), the effect of the metabolic state of a cell upon therapies seeking to produce or silence proteins remains understudied. This project therefore aims to (i) understand whether metabolic perturbations to the mTOR pathway upon PIP3 extracellular administration affect LNP-mediated mRNA delivery, (ii) develop cell- and mouse-agnostic high throughput LNP screening systems for siRNA and DNA drugs that will allow scientists to perform mechanistic studies on functional delivery with genetic knockout mice, and (iii) leverage these platforms to study whether mice exhibiting different levels of activity across the mTOR signaling pathway are more or less receptive to different nucleic acid drugs. This work will constitute early steps toward two equally important goals: (a) exploiting natural differences in cell signaling to improve cell type\u0026ndash;specific nanoparticle delivery and (b) understanding how different physiological states can lead to different delivery potencies of nucleic acid therapeutics.\u0026nbsp;\u003C\/p\u003E\r\n","summary":null,"format":"limited_html"}],"field_subtitle":"","field_summary":[{"value":"\u003Cp\u003EBioE PhD Proposal Presentation-\u0026nbsp; \u0026quot;The impact of the metabolic state of a cell on nucleic acid therapeutics \u0026quot; - Alejandro J. Da Silva Sanchez\u0026nbsp;\u003C\/p\u003E\r\n","format":"limited_html"}],"field_summary_sentence":[{"value":"\u0022The impact of the metabolic state of a cell on nucleic acid therapeutics \u0022"}],"uid":"27917","created_gmt":"2021-01-07 17:05:01","changed_gmt":"2021-01-07 17:05:01","author":"Laura Paige","boilerplate_text":"","field_publication":"","field_article_url":"","field_event_time":{"event_time_start":"2021-01-19T15:00:00-05:00","event_time_end":"2021-01-19T17:00:00-05:00","event_time_end_last":"2021-01-19T17:00:00-05:00","gmt_time_start":"2021-01-19 20:00:00","gmt_time_end":"2021-01-19 22:00:00","gmt_time_end_last":"2021-01-19 22:00:00","rrule":null,"timezone":"America\/New_York"},"extras":[],"groups":[{"id":"65448","name":"Bioengineering Graduate Program"}],"categories":[],"keywords":[{"id":"172056","name":"go-BioE"}],"core_research_areas":[],"news_room_topics":[],"event_categories":[{"id":"1792","name":"Arts and Performance"}],"invited_audience":[{"id":"78761","name":"Faculty\/Staff"},{"id":"78771","name":"Public"},{"id":"78751","name":"Undergraduate students"}],"affiliations":[],"classification":[],"areas_of_expertise":[],"news_and_recent_appearances":[],"phone":[],"contact":[],"email":[],"slides":[],"orientation":[],"userdata":""}}}