{"640952":{"#nid":"640952","#data":{"type":"event","title":"PhD Defense by David Mertz","body":[{"value":"\u003Cp\u003E\u003Cstrong\u003EBME PhD Thesis Defense\u003C\/strong\u003E\u003C\/p\u003E\r\n\r\n\u003Cp\u003E\u003Cstrong\u003EDavid Mertz\u003C\/strong\u003E\u003C\/p\u003E\r\n\r\n\u003Cp\u003E\u0026nbsp;\u003C\/p\u003E\r\n\r\n\u003Cp\u003E\u003Cstrong\u003EDate:\u003C\/strong\u003E Wednesday, November 11, 2020\u003C\/p\u003E\r\n\r\n\u003Cp\u003E\u003Cstrong\u003ETime: \u003C\/strong\u003E1:00PM\u003C\/p\u003E\r\n\r\n\u003Cp\u003E\u003Cstrong\u003EMeeting Link:\u0026nbsp;\u003C\/strong\u003E\u003Ca href=\u0022https:\/\/bluejeans.com\/873836633\u0022\u003Ehttps:\/\/bluejeans.com\/873836633\u003C\/a\u003E\u0026nbsp;\u003C\/p\u003E\r\n\r\n\u003Cp\u003E\u003Cstrong\u003EMeeting ID: \u003C\/strong\u003E873 836 633\u003C\/p\u003E\r\n\r\n\u003Cp\u003E\u0026nbsp;\u003C\/p\u003E\r\n\r\n\u003Cp\u003E\u003Cstrong\u003EAdvisor:\u003C\/strong\u003E\u003C\/p\u003E\r\n\r\n\u003Cp\u003EShuichi Takayama, Ph.D.\u003C\/p\u003E\r\n\r\n\u003Cp\u003E\u0026nbsp;\u003C\/p\u003E\r\n\r\n\u003Cp\u003E\u003Cstrong\u003ECommittee Members:\u003C\/strong\u003E\u003C\/p\u003E\r\n\r\n\u003Cp\u003EHang Lu, Ph.D.\u0026nbsp;\u003C\/p\u003E\r\n\r\n\u003Cp\u003EJohnna Temenoff, Ph.D.\u0026nbsp;\u003C\/p\u003E\r\n\r\n\u003Cp\u003EDarin Olson, M.D., Ph.D.\u0026nbsp;\u003C\/p\u003E\r\n\r\n\u003Cp\u003ECurtis Henry, Ph.D.\u0026nbsp;\u003C\/p\u003E\r\n\r\n\u003Cp\u003E\u003Cbr \/\u003E\r\n\u0026nbsp;\u003C\/p\u003E\r\n\r\n\u003Cp\u003E\u003Cstrong\u003ETitle: \u003C\/strong\u003EInverted Adipose Mammary Microtissues for Early-Stage Tumor Progression\u0026nbsp;Testing\u003C\/p\u003E\r\n\r\n\u003Cp\u003E\u0026nbsp;\u003C\/p\u003E\r\n\r\n\u003Cp\u003E\u003Cstrong\u003EAbstract:\u0026nbsp;\u0026nbsp;\u003C\/strong\u003EStromal adipocytes have been increasingly shown to play a role in multiple stages of breast cancer progression. While new culture models are being developed to test hypotheses about interplay between adipose tissue and tumors, none currently exist that recapitulate invasion from the lumen of the mammary duct through a cell-constructed basement membrane into the stromal compartment. Now, we describe a hanging drop culture-based method for producing novel adipocyte-laden mammary microtissues, with a basal side-in architecture, that show potential in their application to early-stage tumor invasion testing. Interestingly, tissues containing adipocytes in a 1:1 ratio with normal epithelium enhance invasion distance by a triple-negative breast cancer cell line three-fold compared to epithelium-only controls. Testing was also conducted using experimental therapies for disrupting CCL5-mediated paracrine chemotaxis and local fibrotic shifts in the microenvironment. The anti-fibrotic drug pirfenidone shows efficacy in the invasion model by slowing invasion at non-toxic concentrations. The use of primary adipocytes in the current model potentially enables its application to a variety of microenvironment models, including for demographic-based comparisons and comorbidities like obesity and diabetes which may require more precise therapeutic testing.\u0026nbsp;\u003C\/p\u003E\r\n","summary":null,"format":"limited_html"}],"field_subtitle":"","field_summary":"","field_summary_sentence":[{"value":"Inverted Adipose Mammary Microtissues for Early-Stage Tumor Progression Testing"}],"uid":"27707","created_gmt":"2020-11-04 15:35:46","changed_gmt":"2020-11-04 15:35:46","author":"Tatianna Richardson","boilerplate_text":"","field_publication":"","field_article_url":"","field_event_time":{"event_time_start":"2020-11-11T13:00:00-05:00","event_time_end":"2020-11-11T14:00:00-05:00","event_time_end_last":"2020-11-11T14:00:00-05:00","gmt_time_start":"2020-11-11 18:00:00","gmt_time_end":"2020-11-11 19:00:00","gmt_time_end_last":"2020-11-11 19:00:00","rrule":null,"timezone":"America\/New_York"},"extras":[],"groups":[{"id":"221981","name":"Graduate Studies"}],"categories":[],"keywords":[{"id":"100811","name":"Phd Defense"}],"core_research_areas":[],"news_room_topics":[],"event_categories":[{"id":"1788","name":"Other\/Miscellaneous"}],"invited_audience":[{"id":"78761","name":"Faculty\/Staff"},{"id":"78771","name":"Public"},{"id":"174045","name":"Graduate students"},{"id":"78751","name":"Undergraduate students"}],"affiliations":[],"classification":[],"areas_of_expertise":[],"news_and_recent_appearances":[],"phone":[],"contact":[],"email":[],"slides":[],"orientation":[],"userdata":""}}}