{"636798":{"#nid":"636798","#data":{"type":"event","title":"PhD Defense by Kelly Leorah Michie","body":[{"value":"\u003Cp\u003EIn partial fulfillment of the requirements for the degree of\u003C\/p\u003E\r\n\r\n\u003Cp\u003E\u0026nbsp;\u003C\/p\u003E\r\n\r\n\u003Cp\u003EDoctor of Philosophy in Biology\u003C\/p\u003E\r\n\r\n\u003Cp\u003EIn the\u003C\/p\u003E\r\n\r\n\u003Cp\u003ESchool of Biological Sciences\u003C\/p\u003E\r\n\r\n\u003Cp\u003E\u0026nbsp;\u003C\/p\u003E\r\n\r\n\u003Cp\u003E\u003Cstrong\u003EKelly Leorah Michie\u003C\/strong\u003E\u003C\/p\u003E\r\n\r\n\u003Cp\u003E\u0026nbsp;\u003C\/p\u003E\r\n\r\n\u003Cp\u003EWill defend her dissertation\u003C\/p\u003E\r\n\r\n\u003Cp\u003E\u0026nbsp;\u003C\/p\u003E\r\n\r\n\u003Cp\u003E\u003Cstrong\u003EProbing \u003Cem\u003EPseudomonas\u003C\/em\u003E \u003Cem\u003Eaeruginosa\u003C\/em\u003E Physiology During Infection Using \u0026ndash;Omics Techniques, Phenotypic Assays and Mouse models\u003C\/strong\u003E\u003C\/p\u003E\r\n\r\n\u003Cp\u003E\u0026nbsp;\u003C\/p\u003E\r\n\r\n\u003Cp\u003EWednesday, July 8\u003Csup\u003Eth\u003C\/sup\u003E, 2020\u003C\/p\u003E\r\n\r\n\u003Cp\u003E12:00 PM\u003C\/p\u003E\r\n\r\n\u003Cp\u003E\u003Ca href=\u0022https:\/\/bluejeans.com\/515878425\/1890\u0022\u003Ehttps:\/\/bluejeans.com\/515878425\/1890\u003C\/a\u003E\u003C\/p\u003E\r\n\r\n\u003Cp\u003EMeeting ID: 515 878 425\u003C\/p\u003E\r\n\r\n\u003Cp\u003EPasscode: 1890\u003C\/p\u003E\r\n\r\n\u003Cp\u003E\u0026nbsp;\u003C\/p\u003E\r\n\r\n\u003Cp\u003E\u0026nbsp;\u003C\/p\u003E\r\n\r\n\u003Cp\u003E\u003Cstrong\u003EThesis Advisor:\u003C\/strong\u003E\u003C\/p\u003E\r\n\r\n\u003Cp\u003EDr. Marvin Whiteley\u003C\/p\u003E\r\n\r\n\u003Cp\u003ESchool of Biological Sciences\u003C\/p\u003E\r\n\r\n\u003Cp\u003EGeorgia Institute of Technology\u003C\/p\u003E\r\n\r\n\u003Cp\u003E\u0026nbsp;\u003C\/p\u003E\r\n\r\n\u003Cp\u003E\u003Cstrong\u003ECommittee Members:\u003C\/strong\u003E\u003C\/p\u003E\r\n\r\n\u003Cp\u003EDr. Edward Botchwey\u003C\/p\u003E\r\n\r\n\u003Cp\u003ESchool of Biomedical Engineering\u003C\/p\u003E\r\n\r\n\u003Cp\u003EGeorgia Institute of Technology\u003C\/p\u003E\r\n\r\n\u003Cp\u003E\u0026nbsp;\u003C\/p\u003E\r\n\r\n\u003Cp\u003EDr. Sam Brown\u003C\/p\u003E\r\n\r\n\u003Cp\u003ESchool of Biological Sciences\u003C\/p\u003E\r\n\r\n\u003Cp\u003EGeorgia Institute of Technology\u003C\/p\u003E\r\n\r\n\u003Cp\u003E\u0026nbsp;\u003C\/p\u003E\r\n\r\n\u003Cp\u003EDr. Greg Gibson\u003C\/p\u003E\r\n\r\n\u003Cp\u003ESchool of Biological Sciences\u003C\/p\u003E\r\n\r\n\u003Cp\u003EGeorgia Institute of Technology\u003C\/p\u003E\r\n\r\n\u003Cp\u003E\u0026nbsp;\u003C\/p\u003E\r\n\r\n\u003Cp\u003EDr. Joanna B. Goldberg\u003C\/p\u003E\r\n\r\n\u003Cp\u003ESchool of Biological and Biomedical Sciences\u003C\/p\u003E\r\n\r\n\u003Cp\u003EEmory University\u003C\/p\u003E\r\n\r\n\u003Cp\u003E\u0026nbsp;\u003C\/p\u003E\r\n\r\n\u003Cp\u003E\u003Cstrong\u003EABSTRACT\u003C\/strong\u003E\u003C\/p\u003E\r\n\r\n\u003Cp\u003E\u0026nbsp;\u003C\/p\u003E\r\n\r\n\u003Cp\u003EThe opportunistic pathogen \u003Cem\u003EPseudomonas aeruginosa\u003C\/em\u003E causes severe disease in people with compromised immune systems or co-morbidities such as diabetes or cystic fibrosis. Since even intense antibiotic regimens are often ineffective, there is a great need to better understand \u003Cem\u003EP. aeruginosa\u003C\/em\u003E infection biology. Our first research goal was to elucidate the role of glutathione (GSH) biosynthesis for \u003Cem\u003EP. aeruginosa\u003C\/em\u003E during infection. GSH is a major cellular antioxidant that is important for protection from oxidative stress. We found that GSH biosynthesis provides protection against some antimicrobials, such as bleach and ciprofloxacin. We also discovered that GSH biosynthesis provides a modest fitness benefit to \u003Cem\u003EP. aeruginosa\u003C\/em\u003E in a mouse model of acute pneumonia, but not in chronic wound, abscess, and burn wound mouse models. Our second research goal was to characterize the transcriptomic and proteomic signatures of growth rate in \u003Cem\u003EP. aeruginosa\u003C\/em\u003E. Growth rate has significant impacts on cellular physiology, from cell size to stress tolerance. We cultured \u003Cem\u003EP. aeruginosa \u003C\/em\u003Eat four different growth rates using a chemostat, and quantified mRNA and protein abundances using RNA-seq and proteomics mass spectrometry, respectively. We observed modest correlations between mRNA and protein expression. We also discovered that there was greater variation in mRNA expression compared to protein expression, and that mRNA expression was more strongly affected by changes in growth rate. We calculated protein-to-mRNA ratios, or conversion factors, which could be used to more accurately predict protein abundance from RNA-seq data. The information presented in this work may be useful for better understanding, and ultimately treating, \u003Cem\u003EP. aeruginosa\u003C\/em\u003E infections.\u003C\/p\u003E\r\n","summary":null,"format":"limited_html"}],"field_subtitle":"","field_summary":"","field_summary_sentence":[{"value":"Probing Pseudomonas aeruginosa Physiology During Infection Using \u2013Omics Techniques, Phenotypic Assays and Mouse models"}],"uid":"27707","created_gmt":"2020-07-07 21:02:22","changed_gmt":"2020-07-07 21:02:22","author":"Tatianna Richardson","boilerplate_text":"","field_publication":"","field_article_url":"","field_event_time":{"event_time_start":"2020-07-08T13:00:00-04:00","event_time_end":"2020-07-08T15:00:00-04:00","event_time_end_last":"2020-07-08T15:00:00-04:00","gmt_time_start":"2020-07-08 17:00:00","gmt_time_end":"2020-07-08 19:00:00","gmt_time_end_last":"2020-07-08 19:00:00","rrule":null,"timezone":"America\/New_York"},"extras":[],"groups":[{"id":"221981","name":"Graduate Studies"}],"categories":[],"keywords":[{"id":"100811","name":"Phd Defense"}],"core_research_areas":[],"news_room_topics":[],"event_categories":[{"id":"1788","name":"Other\/Miscellaneous"}],"invited_audience":[{"id":"78761","name":"Faculty\/Staff"},{"id":"78771","name":"Public"},{"id":"174045","name":"Graduate students"},{"id":"78751","name":"Undergraduate students"}],"affiliations":[],"classification":[],"areas_of_expertise":[],"news_and_recent_appearances":[],"phone":[],"contact":[],"email":[],"slides":[],"orientation":[],"userdata":""}}}