{"636090":{"#nid":"636090","#data":{"type":"event","title":"Nano@Tech Virtual:Engineering Human Stem Cells for Treating Cardiac Diseases","body":[{"value":"\u003Ch5\u003EXiaojun Lance Lian, Assistant Professor | Departments of Biomedical Engineering \u0026amp; Biology,\u0026nbsp;The\u0026nbsp;Pennsylvania State University and Penn State Cancer Institute | Huck Institutes, Center for Molecular Immunology and Infectious\u0026nbsp;Disease\u003C\/h5\u003E\r\n\r\n\u003Cp\u003E\u003Cstrong\u003EAbstract: \u003C\/strong\u003EHuman pluripotent stem cells (hPSCs) offer the potential to generate large numbers of functional cardiomyocytes from clonal and patient-specific cell sources. Here we show that temporal modulation of Wnt signaling is both essential and sufficient for efficient cardiac induction in hPSCs under defined, growth factor-free conditions. shRNA knockdown of \u0026beta;-catenin during the initial stage of hPSC differentiation fully blocked cardiomyocyte specification, whereas glycogen synthase kinase 3 inhibition at this point enhanced cardiomyocyte generation. Furthermore, sequential treatment of hPSCs with glycogen synthase kinase 3 inhibitors followed by inducible expression of \u0026beta;-catenin shRNA or chemical inhibitors of Wnt signaling produced a high yield of virtually (up to 98%) pure functional human cardiomyocytes from multiple hPSC lines. The robust ability to generate functional cardiomyocytes under defined, growth factor-free conditions solely by genetic or chemically mediated manipulation of a single developmental pathway should facilitate scalable production of cardiac cells suitable for research and regenerative applications.\u003C\/p\u003E\r\n\r\n\u003Cp\u003E\u003Cstrong\u003EBio:\u003C\/strong\u003E Dr. Lance Lian received his PhD in Chemical engineering from University of Wisconsin-Madison in 2012.\u0026nbsp;During his PhD, Dr. Lian\u0026#39;s \u0026ldquo;Cardiomyocyte Differentiation from Human Pluripotent Stem Cells\u0026rdquo; paper was awarded the best biomedical paper in PNAS and the Cozzarelli Prize of the National Academy of Sciences in 2012.\u0026nbsp;Dr. Lian did his postdoc training at Harvard University and Karolinska Institute for stem cell research. After joining Penn State in 2015, Dr. Lian developed the world\u0026rsquo;s first pancreatic cell differentiation method from stem cells for treating diabetes with only small molecules, which makes this production much more cost-effective and efficient.\u0026nbsp;\u003C\/p\u003E\r\n\r\n\u003Cp\u003E\u003Cstrong\u003EHosted by: Graduates In Nanotechnology (GIN) Research Group at Georgia Tech\u003C\/strong\u003E\u003C\/p\u003E\r\n\r\n\u003Cp\u003EAnyone involved in nanotechnology research at Georgia Tech is welcome to join GIN, undergraduates through post-doctorial.\u003C\/p\u003E\r\n\r\n\u003Cp\u003E\u003Cstrong\u003EEmail Quinn Spadola at: quinn.spadola@ien.gatech.edu\u003C\/strong\u003E\u003C\/p\u003E\r\n","summary":null,"format":"limited_html"}],"field_subtitle":"","field_summary":"","field_summary_sentence":[{"value":"Here we show that temporal modulation of Wnt signaling is both essential and sufficient for efficient cardiac induction in hPSCs under defined, growth factor-free conditions. "}],"uid":"27863","created_gmt":"2020-06-09 16:18:29","changed_gmt":"2020-11-10 18:41:13","author":"Christa Ernst","boilerplate_text":"","field_publication":"","field_article_url":"","field_event_time":{"event_time_start":"2020-10-27T13:00:00-04:00","event_time_end":"2020-10-27T14:00:00-04:00","event_time_end_last":"2020-10-27T14:00:00-04:00","gmt_time_start":"2020-10-27 17:00:00","gmt_time_end":"2020-10-27 18:00:00","gmt_time_end_last":"2020-10-27 18:00:00","rrule":null,"timezone":"America\/New_York"},"extras":[],"groups":[{"id":"213791","name":"3D Systems Packaging Research Center"},{"id":"198081","name":"Georgia Electronic Design Center (GEDC)"},{"id":"197261","name":"Institute for Electronics and Nanotechnology"},{"id":"1271","name":"NanoTECH"},{"id":"213771","name":"The Center for MEMS and Microsystems Technologies"}],"categories":[],"keywords":[{"id":"1692","name":"materials"},{"id":"12701","name":"Institute for Electronics and Nanotechnology"},{"id":"107","name":"Nanotechnology"},{"id":"1925","name":"Electrical and Computer Engineering"},{"id":"14325","name":"Electrochemistry"},{"id":"44511","name":"energy storage"},{"id":"185070","name":"Materials Chemistry"},{"id":"185071","name":"Surfaces"},{"id":"13658","name":"Interfaces"},{"id":"87681","name":"thin films"},{"id":"58051","name":"Institute for Materials"},{"id":"12377","name":"Materials Engineering"}],"core_research_areas":[],"news_room_topics":[],"event_categories":[{"id":"10377","name":"Career\/Professional development"},{"id":"1795","name":"Seminar\/Lecture\/Colloquium"}],"invited_audience":[{"id":"78761","name":"Faculty\/Staff"},{"id":"177814","name":"Postdoc"},{"id":"78771","name":"Public"},{"id":"174045","name":"Graduate students"},{"id":"78751","name":"Undergraduate students"}],"affiliations":[],"classification":[],"areas_of_expertise":[],"news_and_recent_appearances":[],"phone":[],"contact":[{"value":"\u003Cp\u003E\u003Ca href=\u0022mailto:dsgottfried@gatech.edu\u0022\u003EDr. David Gottfried\u003C\/a\u003E\u003Cbr \/\u003E\r\nGeorgia Tech IEN\u003Cbr \/\u003E\r\nDeputy Site Director of GT IEN\u003C\/p\u003E\r\n","format":"limited_html"}],"email":[],"slides":[],"orientation":[],"userdata":""}}}