{"635441":{"#nid":"635441","#data":{"type":"event","title":"PhD Proposal by Melissa Lokugamage","body":[{"value":"\u003Cp\u003E\u003Cstrong\u003EMelissa Lokugamage\u003C\/strong\u003E\u003C\/p\u003E\r\n\r\n\u003Cp\u003E\u003Cstrong\u003EBME PhD Proposal Presentation\u003C\/strong\u003E\u003C\/p\u003E\r\n\r\n\u003Cp\u003E\u0026nbsp;\u003C\/p\u003E\r\n\r\n\u003Cp\u003E\u003Cstrong\u003EDate:\u0026nbsp;\u003C\/strong\u003EMay 28th, 2020\u003C\/p\u003E\r\n\r\n\u003Cp\u003E\u003Cstrong\u003ETime:\u0026nbsp;\u003C\/strong\u003E12:00 PM\u003C\/p\u003E\r\n\r\n\u003Cp\u003E\u003Cstrong\u003ELocation:\u0026nbsp;\u003C\/strong\u003E\u003Ca href=\u0022https:\/\/bluejeans.com\/195074330\u0022 target=\u0022_blank\u0022\u003Ehttps:\/\/bluejeans.com\/195074330\u003C\/a\u003E\u003C\/p\u003E\r\n\r\n\u003Cp\u003E\u0026nbsp;\u003C\/p\u003E\r\n\r\n\u003Cp\u003E\u003Cstrong\u003ECommittee Members:\u003C\/strong\u003E\u003C\/p\u003E\r\n\r\n\u003Cp\u003E\u0026nbsp;\u003C\/p\u003E\r\n\r\n\u003Cp\u003EJames E. Dahlman, Ph.D. (Advisor)\u003C\/p\u003E\r\n\r\n\u003Cp\u003EWallace H. Coulter Department of Biomedical Engineering, Georgia Institute of Technology and Emory University\u003C\/p\u003E\r\n\r\n\u003Cp\u003E\u0026nbsp;\u003C\/p\u003E\r\n\r\n\u003Cp\u003EPhilip J. Santangelo, Ph.D.\u003C\/p\u003E\r\n\r\n\u003Cp\u003EWallace H. Coulter Department of Biomedical Engineering, Georgia Institute of Technology and Emory University\u003C\/p\u003E\r\n\r\n\u003Cp\u003E\u0026nbsp;\u003C\/p\u003E\r\n\r\n\u003Cp\u003EEdward Botchwey, Ph.D.\u003C\/p\u003E\r\n\r\n\u003Cp\u003EWallace H. Coulter Department of Biomedical Engineering, Georgia Institute of Technology and Emory University\u003C\/p\u003E\r\n\r\n\u003Cp\u003E\u0026nbsp;\u003C\/p\u003E\r\n\r\n\u003Cp\u003EWilbur Lam, M.D., Ph.D.\u003C\/p\u003E\r\n\r\n\u003Cp\u003EWallace H. Coulter Department of Biomedical Engineering, Georgia Institute of Technology and Emory University\u003C\/p\u003E\r\n\r\n\u003Cp\u003E\u0026nbsp;\u003C\/p\u003E\r\n\r\n\u003Cp\u003EEric Sorscher, M.D.\u003C\/p\u003E\r\n\r\n\u003Cp\u003EDepartment of Pediatrics, Emory University School of Medicine\u003C\/p\u003E\r\n\r\n\u003Cp\u003E\u0026nbsp;\u003C\/p\u003E\r\n\r\n\u003Cp\u003E\u003Cstrong\u003ETitle:\u0026nbsp;\u003C\/strong\u003EUtilizing high-throughput in vivo nanoparticle screens to improve drug delivery\u003C\/p\u003E\r\n\r\n\u003Cp\u003E\u0026nbsp;\u003C\/p\u003E\r\n\r\n\u003Cp\u003E\u003Cstrong\u003EAbstract:\u0026nbsp;\u003C\/strong\u003ELipid-based nanoparticles (LNPs) have emerged as a leading delivery system for nucleic acid therapies and have been used for clinically relevant delivery of RNA-based drugs. Nucleic acids drugs, such as small RNAs, can be utilized to modulate the expression of any gene, making it a powerful tool. While these advances are exciting, systemic delivery of LNP therapies to non-hepatocytes\u0026nbsp;\u003Cem\u003Ein vivo\u003C\/em\u003E\u0026nbsp;remains a challenge. Despite complex\u0026nbsp;\u003Cem\u003Ein vivo\u003C\/em\u003E\u0026nbsp;barriers, nanoparticles are typical screened\u0026nbsp;\u003Cem\u003Ein vitro\u003C\/em\u003E. We have shown that\u0026nbsp;\u003Cem\u003Ein vitro\u003C\/em\u003E\u0026nbsp;screening methods for nanoparticles do not recapitulate\u0026nbsp;\u003Cem\u003Ein vivo\u003C\/em\u003E\u0026nbsp;barriers. To accurately study LNP delivery, taking into account all of the barriers encountered in vivo, and to improve the capacity of RNA therapeutics, we sought to develop a new approach for in vivo nanoparticle discovery, facilitating RNA-based therapies to treat genetic diseases in patients. In this proposal, we sought to designed and utilize improved DNA barcoding systems to simultaneously test hundreds of LNPs\u0026nbsp;\u003Cem\u003Ein vivo\u003C\/em\u003E. First, we create novel LNP screening systems to quantify absolute delivery of nucleic acids. Second, we use our screening systems to test hundreds of unique LNPs\u0026nbsp;\u003Cem\u003Ein vivo\u003C\/em\u003E; we identify novel LNP components and traits that shift delivery away from the liver hepatocytes. Third, we test how alterations to endocytosis genes effected nanoparticle delivery in a cell-type dependent manner. Fourth, we examine the effects of inflammation on delivery. Several disease states include TLR4-mediated inflammatory signaling; our data suggests that TLR4-mediated inflammation inhibits LNP-mediated mRNA delivery. Taken together, this proposal allows us to improve and study LNP delivery to enable gene therapies.\u003C\/p\u003E\r\n","summary":null,"format":"limited_html"}],"field_subtitle":"","field_summary":"","field_summary_sentence":[{"value":"Utilizing high-throughput in vivo nanoparticle screens to improve drug delivery"}],"uid":"27707","created_gmt":"2020-05-18 17:51:37","changed_gmt":"2020-05-18 17:51:37","author":"Tatianna Richardson","boilerplate_text":"","field_publication":"","field_article_url":"","field_event_time":{"event_time_start":"2020-05-28T13:00:00-04:00","event_time_end":"2020-05-28T15:00:00-04:00","event_time_end_last":"2020-05-28T15:00:00-04:00","gmt_time_start":"2020-05-28 17:00:00","gmt_time_end":"2020-05-28 19:00:00","gmt_time_end_last":"2020-05-28 19:00:00","rrule":null,"timezone":"America\/New_York"},"extras":[],"groups":[{"id":"221981","name":"Graduate Studies"}],"categories":[],"keywords":[{"id":"102851","name":"Phd proposal"}],"core_research_areas":[],"news_room_topics":[],"event_categories":[{"id":"1788","name":"Other\/Miscellaneous"}],"invited_audience":[{"id":"78761","name":"Faculty\/Staff"},{"id":"78771","name":"Public"},{"id":"174045","name":"Graduate students"},{"id":"78751","name":"Undergraduate students"}],"affiliations":[],"classification":[],"areas_of_expertise":[],"news_and_recent_appearances":[],"phone":[],"contact":[],"email":[],"slides":[],"orientation":[],"userdata":""}}}