{"634051":{"#nid":"634051","#data":{"type":"event","title":"Microbial Dynamics Seminar at 3pm ","body":[{"value":"\u003Cp\u003EMadeline Mei from Diggle lab will present her work on R-pyocins and competition between\u0026nbsp;\u003Cem\u003EPseudomonas aeruginosa\u003C\/em\u003E strains\u003C\/p\u003E\r\n\r\n\u003Cp\u003ESummary:\u003C\/p\u003E\r\n\r\n\u003Cp\u003E\u003Cstrong\u003ER-Pyocin-mediated killing between Pseudomonas aeruginosa strains isolated from cystic fibrosis lungs\u003C\/strong\u003E\u003C\/p\u003E\r\n\r\n\u003Cp\u003E\u0026nbsp;\u003C\/p\u003E\r\n\r\n\u003Cp\u003E\u003Cem\u003EPseudomonas aeruginosa\u003C\/em\u003E\u0026nbsp;(\u003Cem\u003EPa\u003C\/em\u003E) is a prevalent Gram-negative opportunistic pathogen found in a number of infections; however, it is a major problem in lung infections of individuals with cystic fibrosis (CF). It is believed that one strain of\u0026nbsp;\u003Cem\u003EPa\u003C\/em\u003E\u0026nbsp;takes over and diversifies over the course of these chronic lung infections, but the mechanism for this is poorly understood.\u0026nbsp;R-type pyocins are narrow spectrum, phage tail-like bacteriocins, specifically produced by Pa to kill other strains of Pa.\u0026nbsp;Current studies have shown that R-type pyocins can play a role in intra-species competition due to the production of various types (types 1-5) of R-pyocins, as each strain produces only one of the five R-pyocin types\u003Cem\u003E.\u0026nbsp;\u003C\/em\u003E\u003C\/p\u003E\r\n\r\n\u003Cp\u003E\u0026nbsp;\u003C\/p\u003E\r\n\r\n\u003Cp\u003EUsing our\u0026nbsp;\u0026ldquo;biobank\u0026rdquo; of whole populations of\u0026nbsp;\u003Cem\u003EPa\u003C\/em\u003E\u0026nbsp;from CF patients, we found that (i) populations collected from a single sputum sample produce the same R-pyocin type; (ii) populations from the same sputum sample exhibit diversity in susceptibility to pyocins of other isolates of\u0026nbsp;\u003Cem\u003EPa\u003C\/em\u003E; (iii) differential susceptibility of CF isolates to R-pyocins is due to differences in lipopolysaccharide (LPS). Our work is a step towards understanding how intra-strain competition via pyocins can affect the dynamics of evolved, diverse\u0026nbsp;\u003Cem\u003EPa\u003C\/em\u003E\u0026nbsp;populations.\u003C\/p\u003E\r\n\r\n\u003Cp\u003E\u0026nbsp;\u003C\/p\u003E\r\n\r\n\u003Cp\u003E\u0026nbsp;\u003C\/p\u003E\r\n\r\n\u003Cp\u003E\u0026nbsp;\u003C\/p\u003E\r\n","summary":null,"format":"limited_html"}],"field_subtitle":"","field_summary":"","field_summary_sentence":[{"value":"CMDI Seminar 2020"}],"uid":"35021","created_gmt":"2020-04-03 18:52:03","changed_gmt":"2020-04-05 14:59:44","author":"mavdonina3","boilerplate_text":"","field_publication":"","field_article_url":"","field_event_time":{"event_time_start":"2020-04-06T16:00:00-04:00","event_time_end":"2020-04-06T17:00:00-04:00","event_time_end_last":"2020-04-06T17:00:00-04:00","gmt_time_start":"2020-04-06 20:00:00","gmt_time_end":"2020-04-06 21:00:00","gmt_time_end_last":"2020-04-06 21:00:00","rrule":null,"timezone":"America\/New_York"},"extras":[],"groups":[],"categories":[],"keywords":[],"core_research_areas":[],"news_room_topics":[],"event_categories":[],"invited_audience":[{"id":"78761","name":"Faculty\/Staff"},{"id":"78771","name":"Public"},{"id":"78751","name":"Undergraduate students"}],"affiliations":[],"classification":[],"areas_of_expertise":[],"news_and_recent_appearances":[],"phone":[],"contact":[{"value":"\u003Cp\u003E\u003Ca href=\u0022https:\/\/bluejeans.com\/550042209\u0022\u003Ehttps:\/\/bluejeans.com\/550042209\u003C\/a\u003E\u003C\/p\u003E\r\n\r\n\u003Cp\u003EMeeting ID: 550 042 209\u003C\/p\u003E\r\n\r\n\u003Cp\u003E\u0026nbsp;\u003C\/p\u003E\r\n\r\n\u003Cp\u003E\u0026nbsp;\u003C\/p\u003E\r\n\r\n\u003Cp\u003E\u0026nbsp;\u003C\/p\u003E\r\n\r\n\u003Cp\u003E\u0026nbsp;\u003C\/p\u003E\r\n","format":"limited_html"}],"email":[],"slides":[],"orientation":[],"userdata":""}}}