{"624662":{"#nid":"624662","#data":{"type":"event","title":"PhD Defense by Sandra I. Grijalva                 ","body":[{"value":"\u003Cp\u003E\u003Cstrong\u003ESandra I. Grijalva\u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp;\u003C\/strong\u003E\u003C\/p\u003E\r\n\r\n\u003Cp\u003EBME Ph.D. Defense Presentation\u003C\/p\u003E\r\n\r\n\u003Cp\u003E\u0026nbsp;\u003C\/p\u003E\r\n\r\n\u003Cp\u003E\u003Cstrong\u003EDate:\u003C\/strong\u003E\u0026nbsp;Thursday, August\u0026nbsp;29, 2019\u003C\/p\u003E\r\n\r\n\u003Cp\u003E\u003Cstrong\u003ETime:\u003C\/strong\u003E10:00 am\u003C\/p\u003E\r\n\r\n\u003Cp\u003E\u003Cstrong\u003ELocation:\u003C\/strong\u003E\u0026nbsp;HSRB E-182 (Emory University)\u003C\/p\u003E\r\n\r\n\u003Cp\u003E\u0026nbsp;\u003C\/p\u003E\r\n\r\n\u003Cp\u003E\u003Cstrong\u003EAdvisor:\u003C\/strong\u003E\u0026nbsp;Hee Cheol Cho, PhD (Emory, BME and Pediatrics)\u003C\/p\u003E\r\n\r\n\u003Cp\u003E\u0026nbsp;\u003C\/p\u003E\r\n\r\n\u003Cp\u003E\u003Cstrong\u003ECommittee Members:\u003C\/strong\u003E\u003C\/p\u003E\r\n\r\n\u003Cp\u003EBum-Rak Choi, PhD (Brown University, Medicine)\u003C\/p\u003E\r\n\r\n\u003Cp\u003EFlavio Fenton, PhD (GaTech, Physics)\u003C\/p\u003E\r\n\r\n\u003Cp\u003ERebecca Levit, PhD (Emory, Medicine)\u003C\/p\u003E\r\n\r\n\u003Cp\u003EChunhui Xu, PhD\u0026nbsp;(Emory, Medicine)\u003C\/p\u003E\r\n\r\n\u003Cp\u003E\u0026nbsp;\u003C\/p\u003E\r\n\r\n\u003Cp\u003E\u003Cstrong\u003ETitle:\u003C\/strong\u003E\u0026nbsp;Engineering cardiac biological pacemaker tissues to dissect source-sink mismatch in the heart\u003C\/p\u003E\r\n\r\n\u003Cp\u003E\u0026nbsp;\u003C\/p\u003E\r\n\r\n\u003Cp\u003E\u003Cstrong\u003EAbstract:\u003C\/strong\u003E\u0026nbsp;Each and every heartbeat is initiated from, and driven by, the pacemaker cells in the sinoatrial node (SAN). More than 10 billion cardiac myocytes and non-myocytes make up the heart, but remarkably, it takes only a few thousand pacemaker (\u0026lt;10,000) cells to pace-and-drive the entire heart. Although we have a general understanding of how individual cardiac pacemaker cells beat automatically, there is a lack of understanding in how a few pacemaker cells can drive the beating of the entire heart. This problem, known as a \u0026ldquo;source-sink mismatch\u0026rdquo;, is a fundamental concept that has been difficult to study due to it being painfully low-throughput to study these pacemaker cells. Recently, we have demonstrated conversion of ventricular cardiomyocytes to induced pacemaker cells (iPMs) by singular expression of\u0026nbsp;\u003Cem\u003ETBX18\u003C\/em\u003E. In this thesis we develop a cardiac pacemaker tissue model of the SAN by exploiting the de novo iPMs.\u003C\/p\u003E\r\n\r\n\u003Cp\u003EWe have examined four design principles of the native SAN, i) number of iPMs required to pace a given number of neighboring ventricular myocytes, ii) influence of autonomic nervous system on pacemaking, iii) role of non-myocyte population in pacemaking,\u0026nbsp;and\u0026nbsp;iv) the need for exit pathways. Our 3D model uses patterned cardiac spheroids, by 3D \u0026ndash;printed silicone mold stenciling techniques. We have created a population of iPMs co-cultured with ventricular cardiomyocytes. The major readout is fast, high-resolution optical mapping using a calcium dye.\u003C\/p\u003E\r\n\r\n\u003Cp\u003EThis work demonstrates the\u0026nbsp;ability\u0026nbsp;to\u0026nbsp;reverse-engineer the SAN (eSAN) to i) provide the mechanistic insights on generating sinus rhythm at the tissue level, ii) exploit the insights gained to better engineer biological pacemakers.\u0026nbsp;\u003C\/p\u003E\r\n","summary":null,"format":"limited_html"}],"field_subtitle":"","field_summary":"","field_summary_sentence":[{"value":"Engineering cardiac biological pacemaker tissues to dissect source-sink mismatch in the heart"}],"uid":"27707","created_gmt":"2019-08-16 17:34:20","changed_gmt":"2019-08-16 17:34:20","author":"Tatianna Richardson","boilerplate_text":"","field_publication":"","field_article_url":"","field_event_time":{"event_time_start":"2019-08-29T11:00:00-04:00","event_time_end":"2019-08-29T13:00:00-04:00","event_time_end_last":"2019-08-29T13:00:00-04:00","gmt_time_start":"2019-08-29 15:00:00","gmt_time_end":"2019-08-29 17:00:00","gmt_time_end_last":"2019-08-29 17:00:00","rrule":null,"timezone":"America\/New_York"},"extras":[],"groups":[{"id":"221981","name":"Graduate Studies"}],"categories":[],"keywords":[{"id":"100811","name":"Phd Defense"}],"core_research_areas":[],"news_room_topics":[],"event_categories":[{"id":"1788","name":"Other\/Miscellaneous"}],"invited_audience":[{"id":"78761","name":"Faculty\/Staff"},{"id":"78771","name":"Public"},{"id":"174045","name":"Graduate students"},{"id":"78751","name":"Undergraduate students"}],"affiliations":[],"classification":[],"areas_of_expertise":[],"news_and_recent_appearances":[],"phone":[],"contact":[],"email":[],"slides":[],"orientation":[],"userdata":""}}}